The efficiency of tumor-specific pH-responsive peptide-modified polymeric micelles containing paclitaxel

Zhao, Bing-Xiang; Zhao, Yanping; Huang, Yue; Luo, Lin-Min; Song, Ping; Wang, Xin; Su, Chen; Yu, Kefu; Zhang, Xuan; Zhang, Qiang

doi:10.1016/j.biomaterials.2011.11.078

Cited by 103 publications

(54 citation statements)

References 46 publications

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“…9,10,26 In brief, DSPE-PEG-maleimide mixed with iRGD at a 1:1 molar ratio (iRGD:DSPE-PEG-maleimide = 1:1) in Hepes (pH = 6.5). This reaction mixture was gently stirred at room temperature for 48 hours under nitrogen gas.…”

Section: Cellsmentioning

confidence: 99%

The antitumor activity of a doxorubicin loaded, iRGD-modified sterically-stabilized liposome on B16-F10 melanoma cells: in vitro and in vivo evaluation

Yu¹,

Zhang²,

Luo³

et al. 2013

IJN

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Considering the fact that iRGD (tumor-homing peptide) demonstrates tumor-targeting and tumor-penetrating activity, and that B16-F10 (murine melanoma) cells overexpress both αv integrin receptor and neuropilin-1 (NRP-1), the purpose of this study was to prepare a novel doxorubicin (DOX)-loaded, iRGD-modified, sterically-stabilized liposome (SSL) (iRGD-SSL-DOX) in order to evaluate its antitumor activity on B16-F10 melanoma cells in vitro and in vivo. The iRGD-SSL-DOX was prepared using a thin-film hydration method. The characteristics of iRGD-SSL-DOX were evaluated. The in vitro leakage of DOX from iRGD-SSL-DOX was tested. The in vitro tumor-targeting and tumor-penetrating characteristics of iRGD-modified liposomes on B16-F10 cells were investigated. The in vivo tumor-targeting and tumor-penetrating activities of iRGD-modified liposomes were performed in B16-F10 tumor-bearing nude mice. The antitumor effect of iRGD-SSL-DOX was evaluated in B16-F10 tumor-bearing C57BL/6 mice in vivo. The average particle size of the iRGD-SSL-DOX was found to be 91 nm with a polydispersity index (PDI) of 0.16. The entrapment efficiency of iRGD-SSL-DOX was 98.36%. The leakage of DOX from iRGD-SSL-DOX at the 24-hour time point was only 7.5%. The results obtained from the in vitro flow cytometry and confocal microscopy, as well as in vivo biodistribution and confocal immunofluorescence microscopy experiments, indicate that the tumor-targeting and tumor-penetrating activity of the iRGD-modified SSL was higher than that of unmodified SSL. In vivo antitumor activity results showed that the antitumor effect of iRGD-SSL-DOX against melanoma tumors was higher than that of SSL-DOX in B16-F10 tumor-bearing mice. In conclusion, the iRGD-SSL-DOX is a tumor-targeting and tumor-penetrating peptide modified liposome which has significant antitumor activity against melanoma tumors.

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Section: Cellsmentioning

confidence: 99%

The antitumor activity of a doxorubicin loaded, iRGD-modified sterically-stabilized liposome on B16-F10 melanoma cells: in vitro and in vivo evaluation

Yu¹,

Zhang²,

Luo³

et al. 2013

IJN

Self Cite

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“…71, and also according to our unpublished results). Besides remolding already-existed CPPs, some researchers turned to fabricating novel CPPs rich in histidines for drug delivery [78][79][80][81]. LAH4 is an amphipathic peptide rich in alanines and leucines containing four histidines in the central region of the sequence [78].…”

Section: The Power Of Histidinesmentioning

confidence: 99%

Taming Cell Penetrating Peptides: Never Too Old To Teach Old Dogs New Tricks

Zhang

Gao

2015

Mol. Pharmaceutics

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Cell penetrating peptides (CPPs) have received substantial attention due to their intrinsic property to cross plasma membranes or even as helpers to facilitate the cellular entry of drug molecules, macromolecules, and nanoparticles. Although CPPs and CPP-like peptides provided versatile platforms for drug delivery, their nonselectivity or lack of delivery efficiency is stirring up debates as to the tactics for the optimizing the CPPs themselves. The good news is that, as spurred by the recent progress in the understanding of tumor microenvironment as well as biochemistry and material sciences, we have made attempts in working on perfecting or even "taming" CPPs and CPP-functionalized drug vectors for tumor delivery, and some of them afforded gratifying results. Due to the fact that these peptides are mainly short peptides made up of amino acids (5-30 amino acids), the addition, modification, or replacement of amino acids might lead to surprisingly improved performance. Several novel environment-responsive CPPs or CPP-like peptides have also been discovered. In this review we will discuss the measures taken to harness the power of CPPs and the discovery of environment-responsive peptides with CPP properties.

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“…98 Drug loading to micelles generally depends on upon the physiochemical property of drug, the chemical composition of core forming polymers, and physical state of micelles core. 99 The release is generally affected by temperature, pH, and environment.…”

Section: Micellesmentioning

confidence: 99%

Nanomedicine in Central Nervous System (CNS) Disorders: A Present and Future Prospective

Soni¹,

Ruhela²,

Medhi³

2016

Adv Pharm Bull

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The efficiency of tumor-specific pH-responsive peptide-modified polymeric micelles containing paclitaxel

Cited by 103 publications

References 46 publications

The antitumor activity of a doxorubicin loaded, iRGD-modified sterically-stabilized liposome on B16-F10 melanoma cells: in vitro and in vivo evaluation

The antitumor activity of a doxorubicin loaded, iRGD-modified sterically-stabilized liposome on B16-F10 melanoma cells: in vitro and in vivo evaluation

Taming Cell Penetrating Peptides: Never Too Old To Teach Old Dogs New Tricks

Nanomedicine in Central Nervous System (CNS) Disorders: A Present and Future Prospective

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