The bispectrum is the leading non-Gaussian statistic in large-scale structure, carrying valuable information on cosmology that is complementary to the power spectrum. To access this information, we need to model the bispectrum in the weakly non-linear regime. In this work we present the first two-loop, i.e., next-to-next-to-leading order perturbative description of the bispectrum within an effective field theory (EFT) framework. Using an analytic expansion of the perturbative kernels up to F6 we derive a renormalized bispectrum that is demonstrated to be independent of the UV cutoff. We show that the EFT parameters associated with the four independent second-order EFT operators known from the one-loop bispectrum are sufficient to absorb the UV sensitivity of the twoloop contributions in the double-hard region. In addition, we employ a simplified treatment of the single-hard region, introducing one extra EFT parameter at two-loop order. We compare our results to N-body simulations using the realization-based grid-PT method and find good agreement within the expected range, as well as consistent values for the EFT parameters. The two-loop terms start to become relevant at k ≈ 0.07h Mpc −1 . The range of wavenumbers with percent-level agreement, independently of the shape, extends from 0.08h Mpc −1 to 0.15h Mpc −1 when going from one to two loops at z = 0. In addition, we quantify the impact of using exact instead of Einstein-de-Sitter kernels for the one-loop bispectrum, and discuss in how far their impact can be absorbed into a shift of the EFT parameters.