The eggstraordinary story of how life begins

Parrington, John; Arnoult, Christophe; Fissore, Rafael A.

doi:10.1002/mrd.23083

Cited by 17 publications

(13 citation statements)

References 134 publications

(219 reference statements)

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“…Hence, only spermatozoa exposing a high density of hyaluronic acid receptors on their surface can bind and penetrate the oocyte (Oehninger & Kotze, ). After sperm penetration, a high level of PLCζ protein must be present in the sperm cell to induce oocyte fertilization events (Parrington, Arnoult, & Fissore, ). Of course, additional experiments are necessary to verify the validity of this correlation, which due to the limited amounts of semen, was not possible in the present work.…”

Section: Discussionmentioning

confidence: 99%

The alteration of PLCζ protein expression in unexplained infertile and asthenoteratozoospermic patients: A potential effect on sperm fertilization ability

Rahimizadeh

Topraggaleh

Nasr‐Esfahani

et al. 2019

Molecular Reproduction Devel

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Failed oocyte activation has been observed in unexplained infertile (UI) and asthenoteratozoospermic (AT) men. The deficiency of phospholipase C-zeta (PLCζ) could be a possible reason for such failures and has not been studied yet. We investigated the expression and localization of PLCζ protein in the sperms of patients with UI and AT conditions. The relationships between PLCζ-related parameters with male age, sperm characteristics, DNA integrity, and cellular maturity were assessed.Semen samples were collected from fertile (n = 40), UI (n = 40), and AT (n = 40) men.Subsequently, semen analysis, DNA fragmentation, hyaluronic acid-binding ability, and PLCζ level along with its distribution were evaluated using computer-assisted sperm analyzer, sperm chromatin structure assay (SCSA), hyaluronic acid-binding assay (HBA), western blot analysis and immunofluorescence microscopy, respectively.Unlike SCSA, the values of HBA, and PLCζ expression were significantly reduced in UI and AT patients compared to fertile men, whereas no significant differences were observed among the experimental groups in terms of PLCζ localization patterns. The regression analysis also showed that HBA is the only variable associated with PLCζ levels. Furthermore, the correlation of male age with PLCζ localization in postacrosomal, equatorial, and acrosomal+postacrosomal+equatorial (A+PA+E) patterns, as well as the relation of normal morphology, with the (A+PA+E) pattern, remained in the regression model. Our findings indicated that reduced PLCζ level along with the increased DNA fragmentation and impaired maturation may be possible etiologies of decreased fertilization in the studied subjects. K E Y W O R D Sasthenoteratozoospermia, oocyte activation, PLCζ, unexplained male infertility

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Section: Discussionmentioning

confidence: 99%

The alteration of PLCζ protein expression in unexplained infertile and asthenoteratozoospermic patients: A potential effect on sperm fertilization ability

Rahimizadeh

Topraggaleh

Nasr‐Esfahani

et al. 2019

Molecular Reproduction Devel

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“…In mammals, fluorescent Ca + 2 sensitive dye techniques help confirm that sperm entry causes persistent oscillations of intracellular Ca + 2 in the ooplasm [ 13 ]. The release of Ca + 2 is the common signal of oocyte activation [ 14 ].…”

Section: From Membrane Fusion To Oocyte Activationmentioning

confidence: 99%

“…In mammalian fertilization, the rise of intracellular Ca + 2 depends on the activation of inositol-1,4,5-triphosphate receptors (IP 3 R) located in specialized compartments of the ER membrane [ 14 , 16 , 17 ] (Fig. 1 —normal activation).…”

Section: Ca + 2 Signalingmentioning

confidence: 99%

Sperm-oocyte interplay: an overview of spermatozoon’s role in oocyte activation and current perspectives in diagnosis and fertility treatment

Zafar

2021

Cell Biosci

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The fertilizing spermatozoon is a highly specialized cell that selects from millions along the female tract until the oocyte. The paternal components influence the oocyte activation during fertilization and are fundamental for normal embryo development; however, the sperm-oocyte interplay is in a continuous debate. This review aims to analyze the available scientific information related to the role of the male gamete in the oocyte activation during fertilization, the process of the interaction of sperm factors with oocyte machinery, and the implications of any alterations in this interplay, as well as the advances and limitations of the reproductive techniques and diagnostic tests. At present, both PLCζ and PAWP are the main candidates as oocyte activated factors during fertilization. While PLCζ mechanism is via IP3, how PAWP activates the oocyte still no clear, and these findings are important to study and treat fertilization failure due to oocyte activation, especially when one of the causes is the deficiency of PLCζ in the sperm. However, no diagnostic test has been developed to establish the amount of PLCζ, the protocol to treat this type of pathologies is broad, including treatment with ionophores, sperm selection improvement, and microinjection with PLCζ protein or RNA.

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“…[24][25][26], and how does the fertilized egg initiate a transient elevation in intracellular calcium concentration (Ca 2+ transient) that is necessary for successful activation of embryonic development (for reviews: Ref. [27][28][29][30][31][32][33].…”

Section: Fertiliz Ati On and Ac Tivati On Of De Velopmentmentioning

confidence: 99%

Toward the understanding of biology of oocyte life cycle in Xenopus Laevis: No oocytes left behind

Sato

Tokmakov

2020

Reprod Medicine & Biology

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Background For the past more than 25 years, we have been focusing on the developmental and reproductive biology of the female gametes, oocytes, and eggs, of the African clawed frog Xenopus laevis. Methods The events associated with the life cycle of these cells can be classified into the four main categories: first, oogenesis and cell growth in the ovary during the first meiotic arrest; second, maturation and ovulation that occur simultaneously and result in the acquisition of fertilization competence and the second meiotic arrest; third, fertilization, that is sperm‐induced transition from egg to zygote; and fourth, egg death after spontaneous activation in the absence of fertilizing sperm. Main findings Our studies have demonstrated that signal transduction system involving tyrosine kinase Src and other oocyte/egg membrane‐associated molecules such as uroplakin III and some other cytoplasmic proteins such as mitogen‐activated protein kinase (MAPK) play important roles for successful ovulation, maturation, fertilization, and initiation of embryonic development. Conclusion We summarize recent advances in understanding cellular and molecular mechanisms underlying life cycle events of the oocytes and eggs. Our further intention is to discuss and predict potentially promising impact of the recent findings on the challenges facing reproductive biology and medicine, as well as societal contexts.

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The eggstraordinary story of how life begins

Cited by 17 publications

References 134 publications

The alteration of PLCζ protein expression in unexplained infertile and asthenoteratozoospermic patients: A potential effect on sperm fertilization ability

The alteration of PLCζ protein expression in unexplained infertile and asthenoteratozoospermic patients: A potential effect on sperm fertilization ability

Sperm-oocyte interplay: an overview of spermatozoon’s role in oocyte activation and current perspectives in diagnosis and fertility treatment

Toward the understanding of biology of oocyte life cycle in Xenopus Laevis: No oocytes left behind

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