The electrical properties and topochemistry of bacterial cells

James, A. M.

doi:10.1016/0001-8686(82)80001-8

Cited by 56 publications

(33 citation statements)

References 69 publications

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“…Changes in electrophoretic mobility have also been observed for other bacteria sampled at various growth stages and killed by formaldehyde (Douglas, 1957;James, 1982). The highest absolute electrophoretic mobility values in this study were measured for GS 24 h cultures, for which the electron micrographs showed few cells with intact cell walls.…”

Section: Electrophoretic Mobilitysupporting

confidence: 71%

Modelling of the acid–base properties of two thermophilic bacteria at different growth times

Heinrich¹,

Bremer²,

McQuillan³

et al. 2008

Geochimica et Cosmochimica Acta

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Section: Electrophoretic Mobilitysupporting

confidence: 71%

Modelling of the acid–base properties of two thermophilic bacteria at different growth times

Heinrich¹,

Bremer²,

McQuillan³

et al. 2008

Geochimica et Cosmochimica Acta

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“…mitis strains, in combination with approximately equivalent IEPs and a N/C surface concentration ratio that is even higher than that for S. mitis, suggests that protein may have been masked in the semi-hydrated preparations used in contact angle measurements but not under the fully hydrated zeta potential conditions. Such a possible masking effect would be least likely to influence the XPS measurements because XPS probes more deeply (approximately 5 nm) than do the other techniques (zeta potentials probe at the plane of shear between the bacterial cell and the fluid (James, 1982); contact angles measure the outermost 0.4-0.5 nm of the semi-hydrated bacterial pellet (Bain & Whitesides, 1988)). …”

Section: Xpsmentioning

confidence: 99%

Physico-chemical and structural properties of the surfaces of Peptostreptococcus micros and Streptococcus mitis as compared to those of mutans streptococci, Streptococcus sanguis and Streptococcus salivarius

Mei

Rouxhet²,

Busscher

1992

Journal of General Microbiology

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The surface properties of nine Streptococcus mitis and four Peptostreptococcus micros strains from the oral cavity were examined and compared with a large group of oral streptococci. Zeta potential and contact angle measurements were employed to determine physico-chemical cell surface properties. In addition, elemental surface concentration ratios were obtained via X-ray photoelectron spectroscopy, and surface structures were examined with transmission electron microscopy. The S. mitis and P. micros strains were found to have higher isoelectric points, higher hydrophobicities and higher N/C surface concentration ratios than some other oral streptococci. The combined data suggest that both species possess large amounts of surface protein. All the S. mitis strains displayed abundant surface fibrils in negative staining, but the P. micros strains were devoid of surface appendages indicating that surface protein is present in different forms in the two species. The surfaces of S. mitis and P. micros type strains differed significantly from the other strains examined.

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“…[14,15] When change the culture conditions of microbial cells like growth rate, medium composition, incubation temperature etc., the cell surface components such as the contents of protein, peptidoglycan (PEG), phospholipid and lipopolysaccharide would be influenced, [16] hence the variation of heavy metal binding site on cell surface. In addition, cell pre-treatment will also affect cell surface components and structural, [17,18] and certainly affect the adsorption capacity of bacterial cell to heavy metals. In our previous studies, a heavy metal binding bacterium Pseudomonas putida 5-x was isolated from electroplating effluent as biosorbent, and the characteristics of the cells for removing Cu 2+ and Ni 2+ , such as uptake kinetics, biosorption capacity and the effects of pH, cations and anions have been well documented.…”

Section: Introductionmentioning

confidence: 99%

Optimizing Cell Preparation Technique to Enhance Adsorption Capacity ofPseudomonas Putida5-X to Heavy Metal Ions

Wang¹,

Zhou²,

Zheng³

2005

Journal of Environmental Science and Health, Part A

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A heavy metal accumulation bacterium Pseudomonas putida 5-x isolated from electroplating effluent was used as biosorbent for heavy metal removal from wastewater. In order to compete with physical adsorbent, the optimization of the cell preparation technique for further improving its heavy metal adsorption capacity was conducted. Experimental results showed that the variation of medium compositions and cell growth age, and different cell pre-treating technique would result in the variation of the cell surface components and structural, hence the variation of adsorption capacity of the cell to heavy metal ions. With optimum cell culture conditions and pretreatment technique, the Cu2+ adsorption capacity of the cell was obviously increased from 51.2 mg g-1 to 89.6 mg g-1. The optimised P. putida 5-x cell could be reused at least five cycles to remove heavy metal ions from electroplating effluent with about 95% removal efficiency.

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The electrical properties and topochemistry of bacterial cells

Cited by 56 publications

References 69 publications

Modelling of the acid–base properties of two thermophilic bacteria at different growth times

Modelling of the acid–base properties of two thermophilic bacteria at different growth times

Physico-chemical and structural properties of the surfaces of Peptostreptococcus micros and Streptococcus mitis as compared to those of mutans streptococci, Streptococcus sanguis and Streptococcus salivarius

Optimizing Cell Preparation Technique to Enhance Adsorption Capacity ofPseudomonas Putida5-X to Heavy Metal Ions

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