The electrochemical potential is a key parameter for cell adhesion and proliferation on carbon surface

Periodic polarization duty cycle tunes performance and adhesion of anodic electroactive biofilms

“…Several non-exclusive causes could be proposed: (i) the high current density reached; (ii) the longer period fraction at which EAB50% is under OCP i.e., at low electrode potentials (quickly stabilizing ~ − 0.45 V vs. Ag/AgCl [24]), indeed lower potentials have been shown to decrease the adhesion of single cells of S. oneidensis MR-1, a model electroactive bacterium [36], and adhesion of mammalian cells dramatically decreased when an underlying electrode was poised below its zero charge potential (i.e. with a negatively charged surface), at which point previously adhering cells could even detach [37]; (iii) periodic local pH variations at the electrode/EAB interface following the cycle of current spike (reconnection) and open circuit ─ though the medium was highly buffered; and (iv) a higher shear-stress imposed on EAB50% which develop an uneven morphology (mushroom-like structure) while EABs grown under continuous polarization are flat, as shown in our previous study [24]. Importantly, the electrode surfaces in the present study were flat and smooth, and less detachment would be expected for a rough surface or 3-dimensional porous electrodes due to better biofilm adhesion on rougher surfaces and/or physical retention within pores.…”

Chemical and Process Engineering

“…The surface properties of the material control adhesion processes, so modifications with integrin agonists are used to enhance properties that promote cell adhesion, growth and proliferation (Cirillo et al, 2021). Depending on a surface potential, the material can inhibit or improve adhesion (Guette-Marquet et al, 2022). In addition to the surface properties, the scaffold topography and porosity significantly impact cell adhesion.…”