The electron histochemistry of cystine—containing proteins in thin transverse sections of human hair

Swift, J. A.

doi:10.1111/j.1365-2818.1968.tb00626.x

Cited by 110 publications

(77 citation statements)

References 19 publications

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“…O exame da cutícula pilosa do Bovino 16, através da microscopia eletrônica de transmissão com o uso da téc-nica de Swift (Swift 1967), revelou marcada modificação na morfologia da exocutícula, porém, as alterações foram mínimas ou ausentes na camada A da exocutícula. Foi observada irregularidade da exocutícula e inclusões anormais de cistina no interior da endocutícula.…”

Section: Discussionunclassified

“…Foi observada irregularidade da exocutícula e inclusões anormais de cistina no interior da endocutícula. A técnica de Swift (Swift 1967), sob microscopia eletrônica de transmissão, é considerada como um método histoquímico específico para a detecção do S presente no aminoácido cistina, havendo uma correlação estequiométrica positiva entre a intensidade da coloração e a concentração de S presente. Na cutícula de cabelos tricotiodistróficos, as camadas A e a exocutícula podem estar ausentes ou descontínuas e glóbulos de material eletrodenso (inclusões cistina-positivas anormais) podem ser identificados na camada A e no interior da endocutícula (Gummer et al 1984, Khumalo et al 2005, como vistas na endocutícula do Bovino 16.…”

Section: Discussionunclassified

“…Cortes semifinos foram corados pelo azul de toluidina para exame em microscopia de luz, visando selecionar os pelos para os cortes ultra-finos. Cortes ultra-finos foram colocados em grades de ouro sobre os quais aplicou-se a técnica de Swift (Swift 1967) para o estudo histoquímico-ultra-estrutural do conteúdo de cistina em fotomicrografias (obtidas em microscópio Zeiss-EM 10) de seções transversais das células cuticulares pilosas.…”

Section: Methodsunclassified

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Patogênese das lesões associadas à intoxicação por Ramaria flavo-brunnescens em bovinos

et al. 2009

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RESUMO.-O estudo da patogênese da intoxicação pelo cogumelo Ramaria flavo-brunnescens em bovinos foi realizado através da avaliação retrospectiva de tecidos selecionados de nove casos espontâneos e quatro casos experimentais. Para a investigação da patogênese das lesões observadas na língua, esôfago, casco e cauda, foram avaliadas as alterações histopatológicas e aspectos histoquímicos e histoquímico-ultra-estruturais das lesões. As técnicas histoquímicas utilizadas foram o Tricrômico deMasson e a oxidação seletiva da ceratina (OSC). O estudo histoquímico-ultra-estrutural foi realizado através da técni-ca de Swift sob microscopia eletrônica de transmissão. Os pelos da vassoura da cauda foram examinados sob microscopia de luz polarizada. Todas as alterações observadas nas estruturas ceratinizadas estudadas, mas especialmente nas que sofrem ceratinização dura, revelaram defeitos na ceratinização. Aliando ao estudo morfológico os resultados obtidos através da técnica da OSC e da microscopia eletrônica/técnica de Swift pode-se associar os defeitos na ceratinização a uma redução na quantidade de aminoácidos sulfurados (cistina), principalmente nas estruturas que sofrem ceratinização dura, sendo este provavelmente o principal mecanismo patogenético na intoxicação por R. flavo brunnescens em bovinos.

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Section: Discussionunclassified

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Patogênese das lesões associadas à intoxicação por Ramaria flavo-brunnescens em bovinos

et al. 2009

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“…In order to confirm the assignment of the layers, the TOF-SIMS data were compared to the TEM results in terms of the Cys/Cyc intensity change. In the high magnification TEM image of a single cuticle layer, the image contrast represented the difference in Cys/Cyc contents because methenaminesilver staining mainly reacts to thiol and disulfide moieties [18]. Therefore, the Cys/Cyc content is high in the A-layer and becomes lower in the exocuticle and endocuticle layers [18,23].…”

Section: Fig 2 Comparison Of the Tem Image Of The Hair Surface Crosmentioning

confidence: 99%

“…The specimen was embedded in an Epon802 resin and ultra-thin sections (100 nm) were cut using an ultramicrotome (NOVA Ultratome) equipped with a diamond knife. The specimen was stained with the conventional methenamine-silver staining protocol [18]. TEM observations were conducted using a JEOL JEM-2000FX transmission electron microscope.…”

Section: B Tof-sims and Tem Analysesmentioning

confidence: 99%

Structural Analysis of the Outermost Hair Surface Using TOF-SIMS with C<sub>60</sub> Depth Profiling Technique

Okamoto

Ishikawa

Aoyagi

et al. 2012

e-J. Surf. Sci. Nanotechnol.

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Components on the outermost hair surface were directly analyzed with time-of-flight secondary ion mass spectrometry (TOF-SIMS). The depth profiling of characteristic amino acid components in hair surfaces was performed using a Bi3++ analysis beam combined with a C60 ++ sputtering beam. The depth analysis of a protein bilayer model of zein and casein layers indicated that the damage caused by C60++ sputtering did not influence the TOF-SIMS analysis, therefore, the C60 sputtering is considered to be feasible at an analysis depth of 2 µm from the surface. The depth profiling analysis data for hair surfaces showed oscillating changes in the peak intensities of amino acid fragments, and this phenomenon is considered to reflect the multilayer structure of cuticle tissue. Focusing on the first layer of cuticle tissue, the depth profiling change of the cysteine/cystine-related fragment corresponding to the A-layer, exocuticle and endocuticle of cuticle tissue was consistent with the TEM result. Furthermore, a comparison of the depth profiling data from before and after the bleaching treatment revealed that the cysteine/cystine-related peak intensity was high at the outermost surface because the layer was cysteine-rich for bonding the lipid layer. As a result, the existence of a thin protein layer with specific amino acid components, known as the epicuticle, was suggested. The depth profiling analysis of protein using TOF-SIMS provides significant information for investigating the structure and functions of the surface of human hair.

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Ultrastructure of human scalp hair shafts as revealed by freeze-substitution fixation

Takami

Tajima

Arai³

et al. 1998

Anat. Rec.

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Human scalp hair is important as a diagnostic clue to many diseases, in medical jurisprudential investigations, and also as a subject of cosmetic treatments. While many ultrastructural studies of the human hair root including the hair follicle have been reported, few studies have been done on the human hair shaft. We report here the ultrastructure of human scalp hair shafts prepared by a rapid-freezing technique followed by freeze-substitution fixation that allows the observation of fine cell structures. Healthy scalp hair shafts from Japanese females 12-13 years of age were rapid-frozen and then freeze-substituted in OsO4-acetone. In addition, this technique was applied to the study of some changes of the hair shafts (i.e., hair damaged by thioglycolic acid cold permanent waving and white hair). By this method, the hair shaft was rapid-frozen throughout without appreciable ice damage although the hair shaft was nearly 100 microm in diameter. The rapid-freezing technique resulted in excellent preservation of the ultrastructure of the hair shafts: lamellar structures in the cuticle and fine fibrous ultrastructures in the cortex were observed without chemical treatments. Thioglycolic acid treatment affected the ultrastructure of both the cuticle and the cortex. Except for the absence of melanin granules, no significant differences in the ultrastructure were observed between white hair and black hair. The rapid-freezing technique followed by freeze-substitution fixation appears to be the most reliable approach for the morphological evaluation of fully keratinized cells and tissues.

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The electron histochemistry of cystine—containing proteins in thin transverse sections of human hair

Cited by 110 publications

References 19 publications

Patogênese das lesões associadas à intoxicação por Ramaria flavo-brunnescens em bovinos

Patogênese das lesões associadas à intoxicação por Ramaria flavo-brunnescens em bovinos

Structural Analysis of the Outermost Hair Surface Using TOF-SIMS with C<sub>60</sub> Depth Profiling Technique

Ultrastructure of human scalp hair shafts as revealed by freeze-substitution fixation

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