The Electrophoretic Properties and Some Surface Components of Penicillium Conidia

Fisher, David J.; Richmond, D. V.

doi:10.1099/00221287-64-2-205

Cited by 29 publications

(11 citation statements)

References 39 publications

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“…Wetting of such spores might be prevented by the trapping of air in surface invaginations. Thus the physical conformation of the surface may be sufficient to account for hydrophobic properties (Fisher & Richmond, 1970). Similar factors have been shown to influence the wetting of the leaves of higher plants (Holloway, 1970).…”

Section: J Fisher P J H O L L O W a Y And D V R I C H M O N Dmentioning

confidence: 89%

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Fatty Acid and Hydrocarbon Constituents of the Surface and Wall Lipids of Some Fungal Spores

Fisher

Holloway

Richmond

1972

Journal of General Microbiology

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SUMMARYMicroelectrophoresis has shown surface lipid to be present on conidia of Alternaria tenuis, Botrytis fabae and Neurospora crassa and on sporangiospores of Rhizopus stolonfer. Surface lipid is absent from conidia of Erysiphe cichoracearum, E. graminis, Nectria galligena, Penicillium expansum and Verticillium albo-atrum and sporangiospores of Mucor rouxii. The compositions of the fatty acid and hydrocarbon fractions of the surface and wall lipids from the same organisms are different. The fatty acids are mainly straight-chain and even carbon-numbered. Palmitic and stearic acids predominate. Polyunsaturated acids were absent. Surface hydrocarbons consist almost entirely of n-alkanes but wall fractions are more complex. An equal distribution of odd and even carbon-numbered alkanes occurs in both the surface and wall lipid fractions.

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Section: J Fisher P J H O L L O W a Y And D V R I C H M O N Dmentioning

confidence: 89%

“…By means of this technique surface lipid was detected on conidia of Neurospora crassa, Alternaria tenuis and Botrytis fabae (Somers & Fisher, 1967; Fisher & Richmond, 1969).…”

Section: Introductionmentioning

confidence: 99%

Fatty Acid and Hydrocarbon Constituents of the Surface and Wall Lipids of Some Fungal Spores

Fisher

Holloway

Richmond

1972

Journal of General Microbiology

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“…candidum (arthrospores) were harvested with double-distilled water (30 ml per flask) from cultures grown for 5 d at 25 "C in 250 ml conical flasks containing 50 ml volumes of Vogel's agar medium. To minimize the variation in spore age (Fisher & Richmond, 1970), the cultures were inoculated with a spore suspension which was spread over the surface of the agar medium. Spore suspensions were centrifuged at 1250g (rav, 7.5 cm) for 5 min, and, after removing the supernatant, the spores were suspended in 30 ml sterile double-distilled water.…”

Section: E T H O D Smentioning

confidence: 99%

Effects of Junlon and Hostacerin on the Electrokinetic Properties of Spores of Aspergillus niger, Phanerochaete chrysosporium and Geotrichum candidum

1988

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The electrophoretic behaviour of freshly harvested spores of Aspergillus niger, Phanerochaete chrysosporium and Geotrichum candidum was determined in solution at various pH values. Freshly harvested spores of all three species lacked positive mobility at low pH values, suggesting a preponderance of acidic surface groups. Spores of the 'non-aggregating' fungus, G. candidum, had a pH-mobility curve (peak of negative mobility between pH 3 and 4) which was quite different to those of spores of the 'aggregating' fungi, A . niger and P. chrysosporium. The pH-mobility curve of swollen spores of A . niger which had been incubated in medium differed from that of freshly harvested spores, suggesting that changes in the wall that occur during germination alter the electrophoretic properties of the spores; swollen spores of A . niger, unlike freshly harvested spores, had a negative mobility maximum at pH 5.0.After treatment with Junlon-110 (a polyacrylic acid), spores of all three species had similar pH-mobility curves and all had peaks of negative mobility at pH 4.0. The electrophoretic mobility of spores of P. chrysosporium at pH 6.5 increased linearly with the concentration (0.01-0.4%, w/v) of Junlon used in the pre-treatment ; electrophoretic mobility after pre-treatment with 0.005-0.1 % (w/v) Hostacerin (sodium polyacrylate) increased only up to 0.01 % (w/v) Hostacerin. The results obtained show that Junlon-1 10 and Hostacerin bind to fungal walls, and it is possible that spore and hyphal aggregation is reduced by these compounds because of repulsion between particles resulting from ionized carboxyl groups on the polymer.

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“…Neurospora crassa conidia have been shown to be covered with a layer of regularly arranged fibers, or rodlets, which play a role in conferring water repellency to the conidia and in making them readily dispersed in the air (2,9). Studies on the chemical composition of rodlets from other fungi have been hampered by difficulties in isolation (12), although Hashimoto et al (14) have recently succeeded in isolating rodlet layers from microconidia of Trichophyton mentagrophytes by preparing cell walls and then chemically and enzymatically digesting away all of the wall except the rodlet layer.…”

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Purification and chemical characterization of the rodlet layer of Neurospora crassa conidia

Beever¹,

Redgwell²,

Dempsey³

1979

J Bacteriol

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The rodlet layer of Neurospora crassa macroconidia has been purified and chemically characterized. Sheets of rodlets were released from the conidial surface by vigorously shaking conidia in water. Conidia were removed by filtration and low-speed centrifugation, and the rodlets were recovered from the supernatant by high-speed centrifugation. The rodlet pellet comprised 1.9% of the initial dry weight. Chemical analysis was hampered by the insolubility of the rodlets. They were not solubilized by heating in various protein-denaturing buffers and were only partially dissolved by heating in 1 M NaOH at 100°C for 5 min. Nevertheless, they were found to be largely composed of protein (91%, based on total nitrogen). The major amino acids in acid hydrolysates were aspartic acid, glycine, serine, alanine, half-cystine, and valine. Glucosamine was not detected in acid hydrolysates. The sulfur content was 2.5%, and this could be accounted for in half-cystine and methionine. Carbohydrate comprised just over 2%. The phosphorus content was 0.21%, of which less than one-third was accounted for in phospholipid. The total fatty acid content was 1.0%, most of which could be accounted for by the fatty acids of the phospholipids. 1063 on August 2, 2020 by guest

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The Electrophoretic Properties and Some Surface Components of Penicillium Conidia

Cited by 29 publications

References 39 publications

Fatty Acid and Hydrocarbon Constituents of the Surface and Wall Lipids of Some Fungal Spores

Fatty Acid and Hydrocarbon Constituents of the Surface and Wall Lipids of Some Fungal Spores

Effects of Junlon and Hostacerin on the Electrokinetic Properties of Spores of Aspergillus niger, Phanerochaete chrysosporium and Geotrichum candidum

Purification and chemical characterization of the rodlet layer of Neurospora crassa conidia

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