The ellagitannin HeT induces electrolyte leakage, calcium influx and the accumulation of nitric oxide and hydrogen peroxide in strawberry

Seasonal Variation of Plant Defense Inductor Ellagitannins in Strawberry Leaves under Field Conditions for Phytosanitary Technological Applications

“…In a previous work, we have determined that HeT increased in the leaves of strawberry plants inoculated with an avirulent isolate of Colletotrichum fragariae but not in those challenged with a virulent isolate of Colletotrichum acutatum or in noninoculated plants under controlled conditions . It was also observed that when HeT was sprayed over strawberry plants, it could protect them against a subsequent pathogen attack. ,, This biological activity includes the activation of innate plant immunity associated to molecular and biochemical changes in plant cells such as a fast electrolyte leakage, plasma membrane hyperpolarization, rapid calcium influx, and the accumulation of nitric oxide and hydrogen peroxide in plant cells . Stomatal closure, salicylic acid and callose accumulation, and induction of the PR1 and PDF1.2 (unpublished data) genes were also observed in strawberry plant leaves treated with HeT.…”

“…13 It was also observed that when HeT was sprayed over strawberry plants, it could protect them against a subsequent pathogen attack. 13,23,24 This biological activity includes the activation of innate plant immunity associated to molecular and biochemical changes in plant cells such as a fast electrolyte leakage, plasma membrane hyperpolarization, rapid calcium influx, and the accumulation of nitric oxide and hydrogen peroxide in plant cells. 23 Stomatal closure, salicylic acid and callose accumulation, and induction of the PR1 13 and PDF1.2 (unpublished data) genes were also observed in strawberry plant leaves treated with HeT.…”

“…13,23,24 This biological activity includes the activation of innate plant immunity associated to molecular and biochemical changes in plant cells such as a fast electrolyte leakage, plasma membrane hyperpolarization, rapid calcium influx, and the accumulation of nitric oxide and hydrogen peroxide in plant cells. 23 Stomatal closure, salicylic acid and callose accumulation, and induction of the PR1 13 and PDF1.2 (unpublished data) genes were also observed in strawberry plant leaves treated with HeT. It was further proposed that HeT would be a general elicitor since it also confers protection to another plant species such as Citrus limon against Xanthomonas citri subsp.…”