The embryogenesis of the equine femorotibial joint: The equine interzone

Jenner, Florien; Osch, Gerjo J.V.M. van; Weninger, Wolfgang J.; Geyer, Stefan H.; Stout, T. A. E.; Weeren, René van; Brama, P. A. J.

doi:10.1111/evj.12321

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…HREM is a highly robust microscopic method that is ideal for visualizing a broad spectrum of organic materials used in biomedicine and industry 18 21 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 . It can be employed as an exclusive imaging modality, as currently used by the Mechanisms of Developmental Disorders (DMDD) program 41 42 43 44 , or as an integrative part of multimodal imaging pipelines 45 .…”

Section: Discussionmentioning

confidence: 99%

High-resolution Episcopic Microscopy (HREM) - Simple and Robust Protocols for Processing and Visualizing Organic Materials

Geyer

Maurer‐Gesek

Reissig

et al. 2017

JoVE

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We provide simple protocols for generating digital volume data with the high-resolution episcopic microscopy (HREM) method. HREM is capable of imaging organic materials with volumes up to 5 x 5 x 7 mm3 in typical numeric resolutions between 1 x 1 x 1 and 5 x 5 x 5 µm3. Specimens are embedded in methacrylate resin and sectioned on a microtome. After each section an image of the block surface is captured with a digital video camera that sits on the phototube connected to the compound microscope head. The optical axis passes through a green fluorescent protein (GFP) filter cube and is aligned with a position, at which the bock holder arm comes to rest after each section. In this way, a series of inherently aligned digital images, displaying subsequent block surfaces are produced. Loading such an image series in three-dimensional (3D) visualization software facilitates the immediate conversion to digital volume data, which permit virtual sectioning in various orthogonal and oblique planes and the creation of volume and surface rendered computer models. We present three simple, tissue specific protocols for processing various groups of organic specimens, including mouse, chick, quail, frog and zebra fish embryos, human biopsy material, uncoated paper and skin replacement material.

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Section: Discussionmentioning

confidence: 99%

High-resolution Episcopic Microscopy (HREM) - Simple and Robust Protocols for Processing and Visualizing Organic Materials

Geyer

Maurer‐Gesek

Reissig

et al. 2017

JoVE

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show abstract

“…HREM is a highly robust microscopic method that is ideal for visualizing a broad spectrum of organic materials used in biomedicine and industry 18,21,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40 . It can be employed as an exclusive imaging modality, as currently used by the Mechanisms of Developmental Disorders (DMDD) program 41,42,43,44 , or as an integrative part of multimodal imaging pipelines 45 .…”

Section: Discussionmentioning

confidence: 99%

High-resolution Episcopic Microscopy (HREM) - Simple and Robust Protocols for Processing and Visualizing Organic Materials

Geyer

Maurer‐Gesek

Reissig

et al. 2017

JoVE

View full text Add to dashboard Cite

“…3D visualisation of voluminous fetal material is also possible. In particular, joint development was visualised in horse fetuses [94]. Furthermore, the potential of HREM to analyse the heart morphology of human fetal hearts in the first trimester has been shown [95,96].…”

Section: Embryos/fetuses Of Other Speciesmentioning

confidence: 99%

High-Resolution Episcopic Microscopy (HREM): Looking Back on 13 Years of Successful Generation of Digital Volume Data of Organic Material for 3D Visualisation and 3D Display

Geyer

Weninger

2019

Applied Sciences

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High-resolution episcopic microscopy (HREM) is an imaging technique that permits the simple and rapid generation of three-dimensional (3D) digital volume data of histologically embedded and physically sectioned specimens. The data can be immediately used for high-detail 3D analysis of a broad variety of organic materials with all modern methods of 3D visualisation and display. Since its first description in 2006, HREM has been adopted as a method for exploring organic specimens in many fields of science, and it has recruited a slowly but steadily growing user community. This review aims to briefly introduce the basic principles of HREM data generation and to provide an overview of scientific publications that have been published in the last 13 years involving HREM imaging. The studies to which we refer describe technical details and specimen-specific protocols, and provide examples of the successful use of HREM in biological, biomedical and medical research. Finally, the limitations, potentials and anticipated further improvements are briefly outlined.

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The embryogenesis of the equine femorotibial joint: The equine interzone

Cited by 7 publications

References 18 publications

High-resolution Episcopic Microscopy (HREM) - Simple and Robust Protocols for Processing and Visualizing Organic Materials

High-resolution Episcopic Microscopy (HREM) - Simple and Robust Protocols for Processing and Visualizing Organic Materials

High-resolution Episcopic Microscopy (HREM) - Simple and Robust Protocols for Processing and Visualizing Organic Materials

High-Resolution Episcopic Microscopy (HREM): Looking Back on 13 Years of Successful Generation of Digital Volume Data of Organic Material for 3D Visualisation and 3D Display

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