The Emergence of Carbapenem- and Colistin-Resistant Enterobacteria in Senegal

Sarr, Habibou; Niang, A; Diop, Amadou; Mediannikov, Oleg; Zerrouki, Hanane; Diène, Seydina M.; Lo, Seynabou; Dia, Mamadou Lamine; Sow, A.I.; Fénollar, Florence; Rolain, Jean‐Marc; Hadjadj, Linda

doi:10.3390/pathogens12080974

Cited by 6 publications

References 56 publications

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Genomic analysis of carbapenem- and colistin-resistant Klebsiella pneumoniae complex harbouring mcr-8 and mcr-9 from individuals in Thailand

Hatrongjit,

Wongsurawat,

Jenjaroenpun

et al. 2024

Sci Rep

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The surge in mobile colistin-resistant genes ( mcr ) has become an increasing public health concern, especially in carbapenem-resistant Enterobacterales (CRE). Prospective surveillance was conducted to explore the genomic characteristics of clinical CRE isolates harbouring mcr in 2015–2020. In this study, we aimed to examine the genomic characteristics and phonotypes of mcr-8 and mcr-9 harbouring carbapenem-resistant K. pneumoniae complex (CRKpnC). Polymerase chain reaction test and genome analysis identified CRKpnC strain AMR20201034 as K. pneumoniae (CRKP) ST147 and strain AMR20200784 as K. quasipneumoniae (CRKQ) ST476, harbouring mcr-8 and mcr-9 , respectively. CRKQ exhibited substitutions in chromosomal-mediated colistin resistance genes ( pmrB, pmrC, ramA, and lpxM ), while CRKP showed two substitutions in crrB , pmrB, pmrC, lpxM and lapB . Both species showed resistance to colistin, with minimal inhibitory concentrations of 8 µg/ml for mcr-8 -carrying CRKP isolate and 32 µg/ml for mcr-9 -carrying CRKQ isolate. In addition, CRKP harbouring mcr-8 carried bla NDM , while CRKQ harbouring mcr-9 carried bla IMP , conferring carbapenem resistance. Analysis of plasmid replicon types carrying mcr-8 and mcr-9 showed FIA-FII (96,575 bp) and FIB-HI1B (287,118 bp), respectively. In contrast with the plasmid carrying the carbapenemase genes, the CRKQ carried bla IMP-14 on an IncC plasmid, while the CRKP harboured bla NDM-1 on an FIB plasmid. This finding provides a comprehensive insight into another mcr -carrying CRE from patients in Thailand. The other antimicrobial-resistant genes in the CRKP were bla CTX-M-15 , bla SHV-11 , bla OXA-1 , aac(6′)-Ib-cr, aph(3′)-VI, ARR-3, qnrS1, oqxA, oqxB, sul1, catB3, fosA, and qacE , while those detected in CRKQ were bla OKP-B-15 , qnrA1, oqxA, oqxB, sul1, fosA, and qacE . This observation highlights the importance of strengthening official active surveillance efforts to detect, control, and prevent mcr -harbouring CRE and the need for rational drug use in all sectors.

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Genomic analysis of carbapenem- and colistin-resistant Klebsiella pneumoniae complex harbouring mcr-8 and mcr-9 from individuals in Thailand

Hatrongjit,

Wongsurawat,

Jenjaroenpun

et al. 2024

Sci Rep

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Emergence of mcr-8.1 gene coexisting with blaNDM in Citrobacter werkmanii isolated from a chicken farm in China

Huang,

Cai,

et al. 2024

Journal of Antimicrobial Chemotherapy

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Circulation ofKlebsiella pneumoniaestrains between the termites, chimpanzees and humans

Houmenou,

Sarr,

Diene

et al. 2024

Preprint

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The emergence and multiplication of multi-resistant microbes is becoming a major concern in medicine and scientific research, requiring an integrated, global approach to prevent their spread and develop new therapeutic solutions. Klebsiella pneumoniae can cause a variety of infections. It has several distinctive features, such as extended-spectrum beta-lactamase (ESBL) production and frequency in hospital-acquired infections, which set it apart from other bacteria as Pseudomonas aeruginosa or staphylococci and make it of particular concern in the clinical setting. In this study, we worked on four strains of multidrug-resistant K. pneumoniae isolated from different patients at Fann Hospital in Dakar, Senegal. Whole genomes were compared them with those of nine K. pneumoniae isolates from chimpanzees (CHZ) and six from termites (T_NSC) from our previous study. The comparison revealed two major genetic groups: three human strains were virtually identical to those found in three chimpanzees and four genomes extracted from termites regularly consumed by chimpanzees were identical to five K. pneumoniae strains from chimpanzees. In silico, we identified the resistance genes and their corresponding families from each isolate, then genotyped them before comparing them with other isolates stored in the Institute Pasteur database. These strains have never been previously identified in Senegal. It has been shown here that multiresistant K. pneumoniae strains circulate between chimpanzees and termites and between chimpanzees and humans. Furthermore, these strains, often associated with nosocomial infections, have been identified and genotyped outside Senegal. Although similar strains have been already identified in tropical countries and in different intensive care units throughout the world, we hypothesize that they may originate from termite-chimpanzee ecosystem.

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The Emergence of Carbapenem- and Colistin-Resistant Enterobacteria in Senegal

Cited by 6 publications

References 56 publications

Genomic analysis of carbapenem- and colistin-resistant Klebsiella pneumoniae complex harbouring mcr-8 and mcr-9 from individuals in Thailand

Genomic analysis of carbapenem- and colistin-resistant Klebsiella pneumoniae complex harbouring mcr-8 and mcr-9 from individuals in Thailand

Emergence of mcr-8.1 gene coexisting with blaNDM in Citrobacter werkmanii isolated from a chicken farm in China

Circulation ofKlebsiella pneumoniaestrains between the termites, chimpanzees and humans

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