The emergence of Clostridium difficile PCR-ribotype 001 in Slovakia

Nyč, Otakar; Krutova, Marcela; Líšková, Anna; Matejkova, Jana; Drábek, Jir̆ı́; Kuijper, Ed J.

doi:10.1007/s10096-015-2407-9

Cited by 17 publications

(6 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Before the 2016 study, the C. difficile isolates derived from stool samples of patients hospitalized in Slovakia were characterized in three studies performed between 2011 and 2013. All three studies were in agreement and showed a low diversity of ribotypes, except for a predominance of PCR ribotype 001 (60.1%, 85.0%, 70%) (Davies et al, 2016;Nyc et al, 2015;Freeman et al, 2018).…”

Section: Introductionsupporting

confidence: 70%

“…In 2012, two studies reported the ribotyping data of the Slovak C. difficile isolates, and C. difficile ribotypes 027 and/or 176 were not detected (Davies et al, 2016;Nyc et al, 2015). In contrast, ribotype 001 was identified as predominant in four CDI studies within the period 2012 to 2017 (Davies et al, 2016;Nyc et al, 2015;Freeman et al, 2018;Krehelova et al, 2019) and moreover clonal relatedness of ribotype 001 isolates was confirmed in hospital and between hospitals by multilocus variable number tandem repeats analysis and whole-genome sequencing (Nyc et al, 2015;Krehelova et al, 2019;Eyre et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The emergence of Clostridium difficile ribotypes 027 and 176 with a predominance of the Clostridium difficile ribotype 001 recognized in Slovakia following the European standardized Clostridium difficile infection surveillance of 2016

Nováková

Štefkovičová

Kopilec

et al. 2020

International Journal of Infectious Diseases

View full text Add to dashboard Cite

HighlightsBetween October and December 2016, 36 Slovak hospitals performed Clostridium difficile infection (CDI) surveillance.The overall mean CDI incidence was 2.8 (95% confidence interval 1.9–3.9) cases per 10 000 patient-days.The most prevalent ribotypes were 001 (n = 46, 59.0%) and 176 (n = 23, 29.5%).Almost all isolates showed reduced susceptibility to moxifloxacin (n = 73, 93.6%).A reduced susceptibility to metronidazole was observed in 13 primary fresh isolates.

show abstract

Section: Introductionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

The emergence of Clostridium difficile ribotypes 027 and 176 with a predominance of the Clostridium difficile ribotype 001 recognized in Slovakia following the European standardized Clostridium difficile infection surveillance of 2016

Nováková

Štefkovičová

Kopilec

et al. 2020

International Journal of Infectious Diseases

View full text Add to dashboard Cite

show abstract

“…33 In Germany strains of C. difficile belonging to RT 027 resistant to rifampicin were isolated among patients with osteoarticular infections who were frequently treated with rifampicin. 34 Nyc et al 35 conducted a CDI analysis in 10 academic hospitals in Slovakia by MLVA, testing RT 001 C. difficile fecal isolates of patients from 14 Departments. RT 001, occurred in the years 2007/9 in high percentages in various regions of Europe (Germany, Scotland, Croatia).…”

Section: Dovepressmentioning

confidence: 99%

“…This conclusion is also in line with the conclusions of the above mentioned publications. 28,35,36 C. difficile RT 027 strains are resistant to fluoroquinolones, and possess the MLS B resistance mechanism due to the presence of the ermB gene -multi-drugresistance. 37,38 Among our studied strains, all 29 were resistant to erythromycin (MIC>256µg/mL) and clindamycin (MIC>256µg/mL) and possessed the ermB gene and also were resistant to moxifloxacin.…”

Section: Dovepressmentioning

confidence: 99%

Clostridioides difficile Ribotype 027 (RT027) Outbreak Investigation Due to the Emergence of Rifampicin Resistance Using Multilocus Variable-Number Tandem Repeat Analysis (MLVA)

Kabała¹,

Gofron²,

Aptekorz³

et al. 2021

IDR

View full text Add to dashboard Cite

The aim of this study was Clostridioides difficile outbreak investigation due to the emergence of rifampicin resistant ribotype 027 (RT 027) fecal isolates from patients of Polish tertiary care hospital between X. 2017 and II. 2018 using multilocus variable tandem repeat analysis (MLVA). Materials and Methods: Twenty-nine C. difficile fecal isolates from patients of tertiary care hospital in Southern Poland were ribotyped and analyzed by MLVA. Multiplex PCR (mPCR) for genes encoding GDH (gluD), toxins A (tcdA)/ B (tcdB), 16S rDNA and binary toxin genes (ctdA and ctdB) was performed. The antibiotic susceptibility profile was determined by E-test. Results: The A, B and binary toxins encoding genes were detected in all 29 C. difficile strains which were sensitive to metronidazole, vancomycin and were resistant to erythromycin, clindamycin, and moxifloxacin; resistance to imipenem demonstrated 97%, to rifampicin -45% isolates. C. difficile strains could be grouped by MLVA into 5 distinct clusters, and the largest cluster II contains 16 strains. The comparison of rifampicin GM MIC of cluster II (n=16 strains) with all others (n=13) showed that strains from clusters I, III, IV and V possessed significantly (p <0.005) higher GM MIC and were more resistant to rifampicin. Conclusion: MLVA analysis proved transmission and recognized outbreak due to multidrugresistant RT 027 C. difficile among patients of tertiary care hospital in Southern Poland. The reason for this is probably the widespread occurrence of spores in the hospital environment, which includes, among others, neglect of hygienic procedures and epidemic supervision. High resistance to imipenem (97%) and to rifampicin (45%) among C. difficile RT 027 Silesian isolates is threatening and requires further studies to elucidate this phenomenon.

show abstract

“…This indicated lower antimicrobial resistance levels in countries with a greater C. difficile RT diversity [ 21 ]. In previous CDI studies, RT001 was also identified as predominant in Slovakia [ 22 , 23 ]. The new epidemic strains are less sensitive to antibiotics, e.g., resistant to fluoroquinolones.…”

Section: Discussionmentioning

confidence: 91%

Diagnostic Methods of Clostridioides difficile Infection and Clostridioides difficile Ribotypes in Studied Sample

et al. 2021

View full text Add to dashboard Cite

Background: Clostridioides (Clostridium) difficile is the most common nosocomial pathogen and antibiotic-related diarrhea in health-care facilities. Over the last few years, there was an increase in the incidence rate of C. difficile infection cases in Slovakia. In this study, the phenotypic (toxigenicity, antimicrobial susceptibility) and genotypic (PCR ribotypes, genes for binary toxins) patterns of C. difficile isolates from patients with CDI were analyzed, from July to August 2016, taken from hospitals in the Horne Povazie region of northern Slovakia. The aim of the study was also to identify hypervirulent strains (e.g., the presence of RT027 or RT176). Methods: The retrospective analysis of biological samples suspected of CDI were analyzed by GDH, anaerobic culture, enzyme immunoassay on toxins A/B, multiplex “real-time” PCR and PCR capillary-based electrophoresis ribotyping, and by MALDI TOF MS. Results: C. difficile isolates (n = 44) were identified by PCR ribotyping, which revealed five different ribotypes (RT001, 011, 017, 081, 176). The presence of hypervirulent RT027 was not identified. The C. difficile isolates (RT001, 011, 081, 176) were susceptible to metronidazole and vancomycin. One isolate RT017 had reduced susceptibility to vancomycin. A statistically significant difference between the most prevalent PCR ribotypes, RT001 and RT176, regarding variables such as albumin, CRP, creatinine, the length of hospitalization (p = 0.175), and glomerular filtration (p = 0.05) was not found. Conclusion: The results of PCR capillary-based electrophoresis ribotyping in the studied samples showed a high prevalence of RT176 and 001.

show abstract

The emergence of Clostridium difficile PCR-ribotype 001 in Slovakia

Abstract: We conclude that C. difficile PCR-ribotype 001 is the predominant PCR-ribotype in Slovakia with a strong potential for clonal spread and development of multidrug resistance.

Cited by 17 publications

References 42 publications

The emergence of Clostridium difficile ribotypes 027 and 176 with a predominance of the Clostridium difficile ribotype 001 recognized in Slovakia following the European standardized Clostridium difficile infection surveillance of 2016

The emergence of Clostridium difficile ribotypes 027 and 176 with a predominance of the Clostridium difficile ribotype 001 recognized in Slovakia following the European standardized Clostridium difficile infection surveillance of 2016

Clostridioides difficile Ribotype 027 (RT027) Outbreak Investigation Due to the Emergence of Rifampicin Resistance Using Multilocus Variable-Number Tandem Repeat Analysis (MLVA)

Diagnostic Methods of Clostridioides difficile Infection and Clostridioides difficile Ribotypes in Studied Sample

Contact Info

Product

Resources

About