The emergence of porcine circovirus 2 infections in the Northeastern part of India: A retrospective study from 2011 to 2017

Barman, Nagendra Nath; Nath, Barnali; Kumar, Vishnu; Dutta, Tapan Kumar; Dutta, Biswajit; Rahman, Taibur; Kumar, Sachin

doi:10.1111/tbed.12977

Cited by 14 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The comparison of ORF-2 gene among isolates from this region revealed a nucleotide identity ranging between 90.3% to 100% indicating the genetic diversity of PCV2 strains circulating in pigs of Northwestern region of UP. Latest reports in the north east region of India suggests the circulation of PCV2d and a new cluster similar to current study (Mukherjee et al 2019, Barman et al 2017 The nucleotides with accession numbers LC004754.1 (Sikkim, SK-1), LC008140.1 (Meghalaya, ML-11), LC008139.1 (Assam, AS-3), LC004741.1 (Meghalaya, ML-6), LC004737.1 (Meghalaya, ML-2), LC004740.1 (Meghalaya, ML-5), LC004733.1 (Arunachal Pradesh, AR-2), LC008142.1 (Tripura, TR-1), LC004742.1 (Meghalaya, ML-7), KX009482.1 (Mizoram, PCV2/MZ/IND/43, LC008136.1 (Assam, AS-1), LC004744.1 (Meghalaya, ML-9), LC004743.1 (Meghalaya, ML-8) clustered separately belongs to 2d genotype and KJ729075.1 clustered with various isolates from China with 2d-2 genotype (HM038031.1, KC515005.1, HM038030.1, HM038017.1). In a study by Barman et al (2017) stating the circulation of PCV2d genotype in Meghalaya and Assam.…”

Section: Sample Collectionsupporting

confidence: 87%

Genetic diversity of Indian porcine circovirus type 2 (PCV2) isolates (2006-2018)

John

Kattoor

Sethi

et al. 2020

Indian J of Anim Sci

View full text Add to dashboard Cite

Porcine circovirus type 2 (PCV2) is an emerging viral infection in swine population and results in severe economic loss to piggeries worldwide. The present study was conducted to explore the genetic diversity of PCV2 circulating in swine population of India from 2006–2018. A total of 74 heart, lungs, spleen and lymph nodes collected from different regions in Uttar Pradesh, India were subjected to molecular analysis. For studying genetic diversity, the complete nucleotide and ORF2 sequences of 95 PCV2 including 43 nucleotide sequences from India were used. DNA was extracted from samples and positive samples were subjected to full genome sequencing and phylogenetic analysis was done by maximum likelihood method. Phylogenetic analysis of 40 Indian PCV2 genomes downloaded from GenBank along with three new isolates from the current study based on the complete genome and cap gene together with nucleotide sequences of PCV2 isolates from different countries results in a tree in which Indian isolates clustered in 4 different branches includes PCV2b-1C, PCV2a-2D, PCV 2b-1A/B, PCV 2d-2 recombinant group and two new cluster in which one clustered along with unclassified PCV2 viruses from Indonesia and Croatia. The comparison of ORF2 gene among Indian isolates revealed nucleotide identity ranging from 88.6% to 99.6%, indicating the genetic diversity of PCV2 strains circulating in Indian pig. The present work reports for the first time in India the PCV2-1A/B cluster of 2b genotype and all the Indian isolates available in India from 2006–2018 were used in this analysis.

show abstract

Section: Sample Collectionsupporting

confidence: 87%

Genetic diversity of Indian porcine circovirus type 2 (PCV2) isolates (2006-2018)

John

Kattoor

Sethi

et al. 2020

Indian J of Anim Sci

View full text Add to dashboard Cite

show abstract

“…Prevalence of PCV2b IM1, PCV2d, and PCV2d‐2 genotypes in southern India indicates the PCV2 genotypic shift in circulating strains. Barman et al (2018) documented PCV‐2d genotype associated field infections in northeastern states like Meghalaya, Mizoram, Assam, and Tripura and evidenced PCV2 genotype shifting in northern India. PCV2b‐IM1, PCV2b, PCV2d‐2 and PCV2d genotypes circulating in Tamil Nadu, a state in southern India also allied to that of prevailing global PCV2 genotypic shifting.…”

Section: Discussionmentioning

confidence: 99%

“…Prevalence of PCV2b IM1, PCV2d, and PCV2d-2 genotypes in southern India indicates the PCV2 genotypic shift in circulating strains. Barman et al (2018) Chinese isolates, respectively (Thangthamniyom et al, 2017). PCV2 genotypes circulating in field conditions are essentially to be monitored in order to elucidate the impact of this emerging pathogen in India in comparison to neighbouring countries.…”

Section: Discussionmentioning

confidence: 99%

Emergence of novel Porcine circovirus 2 genotypes in Southern India

Parthiban¹,

Karuppannan²,

et al. 2021

Transbounding Emerging Dis

View full text Add to dashboard Cite

A total of 200 samples from Porcine circovirus 2 suspected (n = 112) and healthy (n = 88) swine populations collected from different districts of Tamil Nadu, south India were used in this study. The samples comprising of serum (n = 124), swabs from natural orifices (n = 52), and postmortem tissues (n = 24). All the samples were processed and subjected to the screening and detection of the PCV2 genome by a specific PCR assay.PCV2 genomes from positive samples were further subjected to genotyping with specifically designed primers for the full-length amplification of the ORF2 gene which codes for capsid protein (Cp) and serves as an epidemiological marker. Randomly, 13 amplified ORF2 genes were sequenced and the aligned sequences were subjected to signature motif analysis and phylogeny in MEGA X. The molecular prevalence of PCV2 infection in Tamil Nadu is 10.5% (n = 21). Signature motif and phylogenetic studies of 13 samples revealed 38.5% (n = 5) presence of each PCV2b intermediate 1(IM1) and PCV2b genotypes, followed by 15.4% (n = 2) PCV2d-2 and 7.7% (n = 1) PCV2d genotypes. The PCV2b-IM1 genotype has a 99.43% sequence homology with Vietnam isolate (JX506730). PCV2b genotypes showed 99.72% sequence identity with Chinese isolate (KX068219). PCV2d-2 genotypes reported in this study have 100% sequence identity with Taiwan isolate (MF169721). PCV2d genotype showed 97.87% sequence identity with Thailand isolate (MF314293). Amino acid analysis of all the 13 full-length ORF2 gene sequences revealed specific mutations in the immune reactive domains of A, B, C, and D. Capsid protein of three PCV2b and five PCV2b IM1 isolates had extra amino acid residue lysine (K) at 234 position of ORF2 similar to PCV2d. For the first time in South India, PCV2b IM1 and PCV2d-2 genotypes are reported. This study evidences the genetic shifts of PCV2 isolates in India and it is analogous to that of global genotypic shift.

show abstract

“…The PCV2 is considered as a major threat to the piggery sector in India (Mukherjee et al ). Molecular characterization and phylogenetic analysis of the PCV2 isolates based on the cap gene diversity among the strains in pig population of Meghalaya and Assam states of India revealed the isolates to be PCV2a, PCV2b‐1c and PCV2d genotypes (Barman et al ; Mukherjee et al ). Similar to earlier reports, the PCV2 reported in the present study was grouped with cluster PCV2d.…”

Section: Discussionmentioning

confidence: 99%

Association ofMyroides odoratimimusin immunocompromized piglets with post weaning multisystemic wasting syndrome

Choudhary

Bera

et al. 2019

J Appl Microbiol

View full text Add to dashboard Cite

Aim To study the association of opportunistic infection due to Myroides odoratimimus in piglets immunocompromised by porcine circovirus type 2 (PCV2) infection. Methods and Results The clinical samples (n = 101) were analysed bacteriologically. The isolates were identified by their phenotypes and MALDI TOF‐MS analysis as Myroides species. The phylogram constructed based on nucleotide sequences of the 16S rRNA gene showed identity (~99%) with the M. odoratimimus isolates. The minimum inhibitory concentration values for antibiotics revealed M. odoratimimus to be resistant against carbapenem, cephalosporins, aminoglycosides and fluoroquinolones. The presence of PCV2 in affected tissue samples was confirmed by amplification of the 565 bp region of ORF2 of the PCV2 genome. The topology of the phylogenetic tree grouped the PCV2 with cluster—2d. Conclusions PCV2 being immunosuppressive in nature might have impaired the immunity thereby increasing the susceptibility of immunocompromised piglets to opportunistic pathogens such as M. odoratimimus leading to disease severity and high mortality. The M. odoratimimus isolates were found to be multidrug resistant and evidenced for uncertain clinical relevance and hence could act as hidden source of public health hazard. Significance and Impact of the Study Myroides odoratimimus is a rarely reported human pathogen. We reported the incidence of infection due to seemingly rare isolates of M. odoratimimus causing an outbreak of pneumonia in piglets. This appears, to the best of authors' knowledge, to be the first outbreak due to Myroides recorded in animal clinical cases described in the literature.

show abstract

The emergence of porcine circovirus 2 infections in the Northeastern part of India: A retrospective study from 2011 to 2017

Cited by 14 publications

References 31 publications

Genetic diversity of Indian porcine circovirus type 2 (PCV2) isolates (2006-2018)

Genetic diversity of Indian porcine circovirus type 2 (PCV2) isolates (2006-2018)

Emergence of novel Porcine circovirus 2 genotypes in Southern India

Association ofMyroides odoratimimusin immunocompromized piglets with post weaning multisystemic wasting syndrome

Contact Info

Product

Resources

About