The emerging regulatory potential of SCFMet30 -mediated polyubiquitination and proteolysis of the Met4 transcriptional activator

Chandrasekaran, Srikripa; Skowyra, Dorota

doi:10.1186/1747-1028-3-11

Cited by 10 publications

(8 citation statements)

References 41 publications

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“…Nutrient metabolism is closely associated with cell cycle and cell division. The essential protein Met4p is associated with methionine biosynthesis, assimilation and uptake, and affects cell division and cell cycle arrest (Chandrasekaran and Skowyra ). Met4p is a leucine zipper transcriptional activator that synthesizes S ‐adenosylmethionine and interacts with SCF Met30 , which induces proteolysis of Met4p in the presence of high levels of methionine (Patton et al .…”

Section: Discussionmentioning

confidence: 99%

A chemogenomic approach to understand the antifungal action of Lichen-derived vulpinic acid

et al. 2016

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Section: Discussionmentioning

confidence: 99%

A chemogenomic approach to understand the antifungal action of Lichen-derived vulpinic acid

et al. 2016

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“…The latter is an ubiquitination complex composed of Skp1, Cdc53, Cdc34, Rbx1 and Met30 as specific components. Models of regulation of cell metabolism by Met4 and its partners have been proposed (Chandrasekaran & Skowyra, 2008).…”

Section: Regulatory Proteinsmentioning

confidence: 99%

Biodiversity in sulfur metabolism in hemiascomycetous yeasts

Hébert¹,

Casarégola²,

Beckerich³

2011

FEMS Yeast Research

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The evolution of the metabolism of sulfur compounds among yeast species was investigated. Differences between species were observed in the cysteine biosynthesis pathway. Most yeast species possess two pathways leading to cysteine production, the transsulfuration pathway and the O-acetyl-serine (OAS) pathway, with the exception of Saccharomyces cerevisiae and Candida glabrata, which only display the transsulfuration pathway, and Schizosaccharomyces pombe, which only have the OAS pathway. An examination of the components of the regulatory network in the different species shows that it is conserved in all the species analyzed, as its central component Met4p was shown to keep its functional domains and its partners were present. The analysis of the presence of genes involved in the catabolic pathway shows that it is evolutionarily conserved in the sulfur metabolism and leads us to propose a role for two gene families which appeared to be highly conserved. This survey has provided ways to understand the diversity of sulfur metabolism products among yeast species through the reconstruction of these pathways. This diversity could account for the difference in metabolic potentialities of the species with a biotechnological interest.

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“…In striking contrast to most studied instances in which SCF-complex ubiquitylation targets substrates for degradation by the 26S proteasome (reviewed in Jonkers and Rep 2009), the ubiquitylation of Met4 by SCF Met30 is not strictly linked to its immediate degradation (Kaiser et al 2000;Flick et al 2004). In the context of its association with SCF Met30 , polyubiquitylated Met4 is stabilized by interactions with its cofactors Cbf1, Met31, and Met32 (Chandrasekaran et al 2006;Chandrasekaran and Skowyra 2008). Conversely, the cofactors associated with Met4-SCF Met30 are polyubiquitylated and targeted for degradation (Ouni et al 2010).…”

Section: Pathway Genesmentioning

confidence: 99%

Regulation of Amino Acid, Nucleotide, and Phosphate Metabolism in Saccharomyces cerevisiae

2012

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Ever since the beginning of biochemical analysis, yeast has been a pioneering model for studying the regulation of eukaryotic metabolism. During the last three decades, the combination of powerful yeast genetics and genome-wide approaches has led to a more integrated view of metabolic regulation. Multiple layers of regulation, from suprapathway control to individual gene responses, have been discovered. Constitutive and dedicated systems that are critical in sensing of the intra- and extracellular environment have been identified, and there is a growing awareness of their involvement in the highly regulated intracellular compartmentalization of proteins and metabolites. This review focuses on recent developments in the field of amino acid, nucleotide, and phosphate metabolism and provides illustrative examples of how yeast cells combine a variety of mechanisms to achieve coordinated regulation of multiple metabolic pathways. Importantly, common schemes have emerged, which reveal mechanisms conserved among various pathways, such as those involved in metabolite sensing and transcriptional regulation by noncoding RNAs or by metabolic intermediates. Thanks to the remarkable sophistication offered by the yeast experimental system, a picture of the intimate connections between the metabolomic and the transcriptome is becoming clear.

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The emerging regulatory potential of SCFMet30 -mediated polyubiquitination and proteolysis of the Met4 transcriptional activator

Cited by 10 publications

References 41 publications

A chemogenomic approach to understand the antifungal action of Lichen-derived vulpinic acid

A chemogenomic approach to understand the antifungal action of Lichen-derived vulpinic acid

Biodiversity in sulfur metabolism in hemiascomycetous yeasts

Regulation of Amino Acid, Nucleotide, and Phosphate Metabolism in Saccharomyces cerevisiae

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