The emerging role and clinical implication of human exonic circular RNA

Lyu, Dongbin; Huang, Shenglin

doi:10.1080/15476286.2016.1227904

Cited by 97 publications

(80 citation statements)

References 58 publications

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“…Functions of circRNAs remain largely unclear. CircRNAs may be generated from exonic or intronic sequence and function as modifiers of parental gene expression . In the current study, we sequenced four circRNAs of interest following a microarray screening.…”

Section: Discussionmentioning

confidence: 99%

Dysregulated circRNAs in plasma from active tuberculosis patients

Gao

et al. 2018

J Cellular Molecular Medi

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Endogenous circular RNAs (circRNAs) have been reported in various diseases. However, their role in active TB remains unknown. The study was aimed to determine plasma circRNA expression profile to characterize potential biomarker and improve our understanding of active TB pathogenesis. CircRNA expression profiles were screened by circRNA microarrays in active TB plasma samples. Dysregulated circRNAs were then verified by qRT‐PCR. CircRNA targets were predicted based on analysis of circRNA‐miRNA‐mRNA interaction. GO and KEGG pathway analyses were used to predict the function of circRNA. ROC curve was calculated to evaluate diagnostic value for active TB. A total of 75 circRNAs were significantly dysregulated in active TB plasma. By further validation, hsa_circRNA_103571 exhibited significant decrease in active TB patients and showed potential interaction with active TB‐related miRNAs such as miR‐29a and miR‐16. Bioinformatics analysis revealed that hsa_circRNA_103571 was primarily involved in ras signalling pathway, regulation of actin cytoskeleton, T‐ and B‐cell receptor signalling pathway. ROC curve analysis suggested that hsa_circRNA_103571 had significant value for active TB diagnosis. Circulating circRNA dysregulation may play a role in active TB pathogenesis. Hsa_circRNA_103571 may be served as a potential biomarker for active TB diagnosis, and hsa_circRNA_103571‐miRNA‐mRNA interaction may provide some novel mechanism for active TB.

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Section: Discussionmentioning

confidence: 99%

Dysregulated circRNAs in plasma from active tuberculosis patients

Gao

et al. 2018

J Cellular Molecular Medi

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“…The expression of circRNAs is tissue-specific, and most circRNAs exhibit differential expression patterns in different species, tissues and cells (5,6). The majority of circRNAs are <1,500 nucleotides in length, with an average length of ~500 nucleotides (7,8). Several circRNAs have been identified to be closely related to the recurrence and prognosis of bladder cancer.…”

Section: Introductionmentioning

confidence: 99%

circ_0067934 increases bladder cancer cell proliferation, migration and invasion through suppressing miR‑1304 expression and increasing Myc expression levels

Liu

Zhou²,

Zhong³

2020

Exp Ther Med

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circRNAs have been demonstrated to be key regulators of bladder cancer progression. The present study aimed to investigate the effects of circular RNA (circ)_0067934 in bladder cancer progression. A total of 54 patients with primary bladder cancer were enrolled, and their tumor tissues and adjacent normal bladder tissues were collected. For in vitro functional assays, T24 cells were transfected with sicirc_0067934, and Cell Counting Kit-8 was used to analyze the proliferative capacity of T24 cells. In addition, Transwell and Matrigel assays were used to assess the cell migration and invasion abilities, and a dual-luciferase reporter assay was used to investigate the relationship between miR-1304 and circ_0067934. Finally, reverse transcription-quantitative PCR and western blotting were performed to analyze gene and protein expression levels, respectively. circ_0067934 expression levels were significantly increased in bladder cancer tissues (P<0.001), which was associated with metastasis and a significantly decreased 5-year overall (P<0.05) and disease-free survival (P<0.05). In vitro, T24 cells in the small interfering RNA (si)circ_0067934 group demonstrated significantly reduced proliferation, migration and invasion abilities compared with the si negative control (siNC) group (P<0.01). In addition, the knockdown of circ_0067934 directly increased microRNA (miR)-1304 expression levels in T24 cells. Myc was subsequently discovered to be directly inhibited by miR-1304 and circ_0067934 was observed to increase Myc expression levels in T24 cells through inhibiting miR-1304 expression levels (P<0.01). Compared with the siNC group and sicirc_0067934 + Myc overexpression group, T24 cells in the sicirc_0067934 group exhibited significantly decreased proliferative, migratory and invasive abilities (P<0.01). In conclusion, circ_0067934 was demonstrated to increase bladder cancer cell proliferation, migration and invasion through promoting Myc expression levels via the suppression of miR-1304 expression.

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“…circRNAs are single‐stranded covalently closed transcripts. Microarray or RNA sequencing analysis showed that circRNAs are widely dysregulated in cancers and potentially play suppressive or oncogenic roles in multiple cancers . circRNAs could be enriched and stable in exosomes.…”

Section: Oncogenic Roles Of Exosomes In Cancermentioning

confidence: 99%

“…Microarray or RNA sequencing analysis showed that cir-cRNAs are widely dysregulated in cancers and potentially play suppressive or oncogenic roles in multiple cancers. 112 circRNAs could be enriched and stable in exosomes. Exosomes containing tumor-specific circRNAs are released by tumor cells.…”

Section: Exosomal Mirnas Circrnas and Lncrnas In Cancersmentioning

confidence: 99%

Exosomes and cancer: From oncogenic roles to therapeutic applications

et al. 2019

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Exosomes belong to extracellular vehicles that were produced and secreted from most eukaryotic cells and are involved in cell‐to‐cell communications. They are an effective delivery system for biological compounds such as mRNAs, microRNAs (miRNAs), proteins, lipids, saccharides, and other physiological compounds to target cells. In this way, they could influence on cellular pathways and mediate their physiological behaviors including cell proliferation, tumorigenesis, differentiation, and so on. Many research studies focused on their role in cancers and also on potentially therapeutic and biomarker applications. In the current study, we reviewed the exosomes' effects on cancer progression based on their cargoes including miRNAs, long noncoding RNAs, circular RNAs, DNAs, mRNAs, proteins, and lipids. Moreover, their therapeutic roles in cancer were considered. In this regard, we have given a brief overview of challenges and obstacles in using exosomes as therapeutic agents.

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The emerging role and clinical implication of human exonic circular RNA

Cited by 97 publications

References 58 publications

Dysregulated circRNAs in plasma from active tuberculosis patients

Dysregulated circRNAs in plasma from active tuberculosis patients

circ_0067934 increases bladder cancer cell proliferation, migration and invasion through suppressing miR‑1304 expression and increasing Myc expression levels

Exosomes and cancer: From oncogenic roles to therapeutic applications

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