The emerging role of long non-coding RNAs in renal cell carcinoma progression and clinical therapy via targeting metabolic regulation

Gao, Xin; Zhang, Haiying; Zhang, Chang; Li, Minghe; Yu, Xiao; Sun, Yanan; Shi, Yingai; Zhang, Hongxia; He, Xu

doi:10.3389/fphar.2023.1122065

Cited by 4 publications

(2 citation statements)

References 113 publications

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“…The pathogenesis of lncRNA in RCC has been extensively studied. As one of the most researched genes, lncRNA regulates the biological characteristics of cancer, such as cell proliferation, migration, invasion, and apoptosis, through the lncRNA-miRNA-messenger RNA (mRNA) axis ( 21 ). Small non-coding RNA (sncRNA) is a series of RNA molecules approximately 19 to 25 nucleotides in length.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA GPRC5D-AS1 in renal cell carcinoma: a molecular mechanism study

Jia,

Chen,

Kang

et al. 2024

Transl Androl Urol

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Background Clear cell renal cell carcinoma (RCC) is the most common subtype of RCC. Although targeted therapy can provide superior treatment outcomes, it is prone to drug resistance, and individual responses to immunotherapy vary greatly. Therefore, finding new diagnostic and therapeutic targets for RCC is of considerable importance. Long noncoding RNA (lncRNA) GPRC5D-AS1 can serve as a biomarker in clinical applications and the prognosis of lung squamous cell carcinoma. However, the specific mechanism of action of lncRNA GPRC5D-AS1 in RCC has not yet been clarified. Therefore, this paper explores the expression of lncRNA GPRC5D-AS1 in the renal cancer cell line 786-0, and conducts a preliminary study of its molecular mechanism. Selecting nude mice for tumor experiments is because of the high genomic and physiological similarity between mice and humans. Conducting tumor research on mice allows for better control of experimental conditions, aiding researchers in more accurately observing and analysing tumor characteristics and responses. Methods Small interfering RNA (siRNA) and plasmid cloning DNA (pcDNA) 3.1 were used to transfect renal cancer cell line 786-0 to silence and overexpress the lncRNA GPRC5D-AS1 gene. Quantitative real-time fluorescence polymerase chain reaction was used to detect the difference in lncRNA GPRC5D-AS1 expression in blank control group, negative control group, siGPRC5D-AS1 group and oeGPRC5D-AS1 group. The effects of silence and overexpression of lncRNA GPRC5D-AS1 1 on the proliferation of 786-0 cells were detected in cell colony formation experiments; the changes in the migration and invasion of 786-0 cells were detected via cell scratch assay and transwell assay, respectively; the differences in tumor growth between groups were determined via tumorigenesis experiments in nude mice; and the expression of proliferation-related protein [β-catenin, Ki67 and proliferating cell nuclear antigen (PCNA)] and invasion-related protein (N-cadherin and E-cadherin) were detected via Western blotting. Results Compared with blank control group and negative control group, the siGPRC5D-AS1 group showed a significant decrease in the relative expression of lncRNA GPRC5D-AS1 (P<0.05), a significant increase in the number of proliferating cells and migrating cells (P<0.05), a significant increase in the tumor volume of nude mice (P<0.05), a significant increase in β-catenin, Ki67, PCNA and N-cadherin protein expression (P<0.05), and a significant decrease in E-cadherin protein expression (P<0.05); conversely, these results were opposite for the eGPRC5D-AS1 group. Conclusions Silencing the expression of lncRNA GPRC5D-AS1 can enhance the proliferation, invasion, and migration ability of renal ...

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Section: Discussionmentioning

confidence: 99%

Long noncoding RNA GPRC5D-AS1 in renal cell carcinoma: a molecular mechanism study

Jia,

Chen,

Kang

et al. 2024

Transl Androl Urol

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“…Long non-coding RNAs (lncRNAs) have emerged as promising biomarkers, since they modulate genes at various levels, from epigenetics to post-transcription [11]. In line with other recent publications [12,13], the comprehensive review by Rysz and colleagues describes the roles of specific lncRNAs in RCC and suggests their potential as diagnostic and prognostic markers. Understanding the intricate mechanisms by which lncRNAs influence the disease progression in RCC is essential for the development of targeted therapies that can improve patient outcome [14].…”

mentioning

confidence: 88%

Molecular Mechanisms of Tumor Progression and New Therapeutic Strategies for Urological Cancers

Ruiz de Porras,

Font

2023

IJMS

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Circ-IP6K2 suppresses tumor progression by modulating the miR-1292-5p/CAMK2N1 signal in clear cell renal cell carcinoma

Tang,

Yang,

et al. 2024

Funct Integr Genomics

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The emerging role of long non-coding RNAs in renal cell carcinoma progression and clinical therapy via targeting metabolic regulation

Cited by 4 publications

References 113 publications

Long noncoding RNA GPRC5D-AS1 in renal cell carcinoma: a molecular mechanism study

Long noncoding RNA GPRC5D-AS1 in renal cell carcinoma: a molecular mechanism study

Molecular Mechanisms of Tumor Progression and New Therapeutic Strategies for Urological Cancers

Circ-IP6K2 suppresses tumor progression by modulating the miR-1292-5p/CAMK2N1 signal in clear cell renal cell carcinoma

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