The emerging role of small RNAs in ovule development, a kind of magic

Cucinotta, Mara; Mendes, Marta Adelina; Underwood, Charles J; Colombo, Lucia

doi:10.1007/s00497-021-00421-4

Cited by 12 publications

(11 citation statements)

References 123 publications

(230 reference statements)

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“…2q-x). Therefore, different from previously studied regulators [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] , our results suggest that ARF17 is a key determinant for promoting the MMC specification instead of the MMC proliferation. Collectively, we uncover a molecular mechanism underlying the MMC fate determination, which thus lays the foundation to female germline in plants.…”

Section: Discussioncontrasting

confidence: 99%

“…In addition, trans-acting small interfering RNAs known as tasiR-ARFs repress the expression of ARF3 in cells neighboring the MMC to inhibit the formation of ectopic MMCs 11,12 . Moreover, the epigenetic regulation associated with AGO9 (ARGONAUTE9), RDR6 (RNA-DEPENDENT RNA POLYMERASE 6), DRM (DOMAINS REAR-RANGED METHYLASE), RNA helicase gene MEM (MNEME), and SPL/NZZ (SPOROCYTELESS/NOZZLE), might use the similar mechanism to control the number of MMC [13][14][15][16][17][18][19][20][21][22] . Although significant progress has been made toward understanding pathways that restrict MMC formation, the molecular mechanism underlying the promotion of MMC identity remains elusive.…”

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confidence: 99%

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Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis

et al. 2022

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Germline determination is essential for species survival and evolution in multicellular organisms. In most flowering plants, formation of the female germline is initiated with specification of one megaspore mother cell (MMC) in each ovule; however, the molecular mechanism underlying this key event remains unclear. Here we report that spatially restricted auxin signaling promotes MMC fate in Arabidopsis. Our results show that the microRNA160 (miR160) targeted gene ARF17 (AUXIN RESPONSE FACTOR17) is required for promoting MMC specification by genetically interacting with the SPL/NZZ (SPOROCYTELESS/NOZZLE) gene. Alterations of auxin signaling cause formation of supernumerary MMCs in an ARF17- and SPL/NZZ-dependent manner. Furthermore, miR160 and ARF17 are indispensable for attaining a normal auxin maximum at the ovule apex via modulating the expression domain of PIN1 (PIN-FORMED1) auxin transporter. Our findings elucidate the mechanism by which auxin signaling promotes the acquisition of female germline cell fate in plants.

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Section: Discussioncontrasting

confidence: 99%

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confidence: 99%

Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis

et al. 2022

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“…Therefore, TGS1 appears to be a candidate for the genetic control of critical fate decisions that govern female germline development in plants. Our findings open the wedge for investigating how the interplay between TGS1 and key pathways for plant reproduction involved in small RNAs, phytohormones regulation, and cell–cell communication (Lora et al ., 2019; Pinto et al ., 2019; Petrella et al ., 2021) regulates cell fate decision during plant female reproduction.…”

Section: Discussionmentioning

confidence: 99%

TRIMETHYLGUANOSINE SYNTHASE1 mutations decanalize female germline development in Arabidopsis

Siena,

Michaud,

Selles

et al. 2023

New Phytologist

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Summary Here, we report the characterization of a plant RNA methyltransferase, orthologous to yeast trimethylguanosine synthase1 (Tgs1p) and whose downregulation was associated with apomixis in Paspalum grasses. Using phylogenetic analyses and yeast complementation, we determined that land plant genomes all encode a conserved, specific TGS1 protein. Next, we studied the role of TGS1 in female reproduction using reporter lines and loss‐of‐function mutants in Arabidopsis thaliana. pAtTGS1:AtTGS1 reporters showed a dynamic expression pattern. They were highly active in the placenta and ovule primordia at emergence but, subsequently, showed weak signals in the nucellus. Although expressed throughout gametophyte development, activity became restricted to the female gamete and was also detected after fertilization during embryogenesis. TGS1 depletion altered the specification of the precursor cells that give rise to the female gametophytic generation and to the sporophyte, resulting in the formation of a functional aposporous‐like lineage. Our results indicate that TGS1 participates in the mechanisms restricting cell fate acquisition to a single cell at critical transitions throughout the female reproductive lineage and, thus, expand our current knowledge of the mechanisms governing female reproductive fate in plants.

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“…In addition, DEGs were searched for sRNA biogenesis, which is known to play pivotal roles in reproductive development [ 54 , 55 ], and oxidative stress-related genes, which negatively affect reproductive development in plants [ 56 ]. DEGs associated with tryptophan and ethylene metabolism were investigated, which are associated with the biosynthesis and regulation of the phytohormone auxin, a vital component of plant reproduction since it regulates both male and female reproductive organs [ 57 , 58 ].…”

Section: Methodsmentioning

confidence: 99%

Ovule Transcriptome Analysis Discloses Deregulation of Genes and Pathways in Sexual and Apomictic Limonium Species (Plumbaginaceae)

Caperta

Fernandes

Conceição

et al. 2023

Genes

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The genus Limonium Mill. (sea lavenders) includes species with sexual and apomixis reproductive strategies, although the genes involved in these processes are unknown. To explore the mechanisms beyond these reproduction modes, transcriptome profiling of sexual, male sterile, and facultative apomictic species was carried out using ovules from different developmental stages. In total, 15,166 unigenes were found to be differentially expressed with apomictic vs. sexual reproduction, of which 4275 were uniquely annotated using an Arabidopsis thaliana database, with different regulations according to each stage and/or species compared. Gene ontology (GO) enrichment analysis indicated that genes related to tubulin, actin, the ubiquitin degradation process, reactive oxygen species scavenging, hormone signaling such as the ethylene signaling pathway and gibberellic acid-dependent signal, and transcription factors were found among differentially expressed genes (DEGs) between apomictic and sexual plants. We found that 24% of uniquely annotated DEGs were likely to be implicated in flower development, male sterility, pollen formation, pollen-stigma interactions, and pollen tube formation. The present study identifies candidate genes that are highly associated with distinct reproductive modes and sheds light on the molecular mechanisms of apomixis expression in Limonium sp.

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The emerging role of small RNAs in ovule development, a kind of magic

Cited by 12 publications

References 123 publications

Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis

Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis

TRIMETHYLGUANOSINE SYNTHASE1 mutations decanalize female germline development in Arabidopsis

Ovule Transcriptome Analysis Discloses Deregulation of Genes and Pathways in Sexual and Apomictic Limonium Species (Plumbaginaceae)

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