The Emission Soot of Biomass Fuels Combustion as a Source of Endocrine Disrupters

Wu, Wenzhong; Wang, J X; Zhao, Guanghui; You, Liu

doi:10.1081/ese-120003239

Cited by 18 publications

(10 citation statements)

References 26 publications

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“…For example, estrogenic activity of the sample extract was calibrated with a serial dilution of 17β-estrodiol (E2). The E2-equivalent (E2-EQ) concentration was calculated as described by Wu et al [27]. The equivalent concentrations for AR, PR, ERR, and AHR agonistic and antagonistic effects were determined in a similar manner, and their correspondent reference materials are listed in Table 1.…”

Section: Discussionmentioning

confidence: 99%

In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water of different treatment processes

Rao

et al. 2013

Front. Environ. Sci. Eng.

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Effluents from wastewater treatment plant (WWTP) have been reported to have a broad spectrum of endocrine disrupting compounds (EDCs). The majority of studies have focused on the occurrence of estrogenic activity, while ignoring nuclear hormone receptors (NRs) pathways. In the present study, a battery of in vitro yeast bioassays and a cell bioassay, including antagonistic and agonistic effects on estrogen receptor (ER), androgen receptor (AR), progesterone receptor (PR), estrogenrelated receptor (ERR) and aryl hydrocarbon receptor (AHR), were conducted to evaluate the removal efficiencies of EDCs by different treatment processes of a WWTP located in Beijing. Estrogenic, anti-estrogenic, antiandrogenic, anti-progesteronic, anti-ERR and the activation of AHR activities were detected in samples from all treatment processes and the receiving water. The concentration of estrogenic contaminants with estradiol (E 2 ) equivalent concentrations ranged from 0.82 Â 10

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Section: Discussionmentioning

confidence: 99%

In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water of different treatment processes

Rao

et al. 2013

Front. Environ. Sci. Eng.

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“…The extract was carefully added on a column containing 10 g silica (60-200 lm, 60A, ultra pure, Acros Organics) and 10 g Al 2 O 3 (50-200 Micron, Acros Organics) and then fractionated as described by Wu et al (2002) with some modifications. Briefly, the column was eluted with 15 mL hexane (HPLC Grade, Fisher) to yield a non-polar fraction (F1), followed by 70 mL mixture of hexane/CH 2 Cl 2 (v/v=7:3, HPLC Grade, Fisher) to afford a fraction with moderate polarity (F2), and finally by methanol (30 mL, HPLC Grade, Fisher) to produce a polar fraction (F3).…”

Section: Fractionation Based On Polaritymentioning

confidence: 99%

“…The yeast assay was carried out as described by Routledge and Sumpter (1996) and Wu et al (2002) with some modifications.…”

Section: Yeast Assaymentioning

confidence: 99%

“…The b-galactosidase activity was calculated according to the following equations: u ¼ C S =t Á V Á D Á OD S and C S ¼ 10 À6 ðA S À A B Þ=e Á d where u: bgalactosidase activity; t: incubation duration of the enzyme reaction; V: Volume of the test culture; D: dilution factor; OD S : OD 600 of test culture; A S : OD 420 of the enzyme reaction supernatant of the sample; A B : OD 420 of the enzyme reaction supernatant of the blank; e: e for o-nitrophenol in the enzyme assay reaction mix; and d: diameter of the cuvette (Routledge and Sumpter, 1996;Gaido et al 1997). The estradiol equivalents (EEQ) values were calculated as described by Wu et al (2002), comparing the estrogenic activity of the sample extracts with the series concentrations of E2 standard.…”

Section: Yeast Assaymentioning

confidence: 99%

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Assessing the Detoxication Efficiencies of Wastewater Treatment Processes Using a Battery of Bioassays/Biomarkers

Wang

2005

Arch Environ Contam Toxicol

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Abstract. A battery of in vitro bioassays, including a Neutral Red (NR) assay using MCF-7 cells for predicting cytotoxic chemicals, an ethoxy resorufin-O-deethylase (EROD) activity assay using H4IIE cells to check for dioxin-like chemicals, and a recombinant gene yeast assay for screening estrogenic chemicals, was conducted to assess the removal efficiencies of trace toxic chemicals by different treatment processes in the waste water treatment plant (WWTP). The effluents were extracted by solid phase extraction (SPE) and were fractionated into three fractions based on polarities. The battery of bioassays was performed for each fraction. In the battery, the toxicities of the effluents were described according to their modes of actions (MOA) or biomarkers and the properties of the toxic chemicals were categorized by their polarities and MOAs. The proposed procedure could be used as a tool to diagnose the toxic characteristics of the complicate mixture. The results showed that cytotoxic, dioxin-like and estrogenic chemicals could be detected in all samples. In the influent, cytotoxic and dioxin-like chemicals were mainly in polar fraction and estrogenic chemicals were in non-polar and moderate-polar fractions. The secondary treatment (active sludge) could remove a small amount of these toxicants. Among different types of advanced treatments, flocculation was good enough to remove most of the cytotoxic chemicals and a combination of flocculation, ozone oxidation, and post-biological treatment could eliminate most of the dioxin-like and estrogenic chemicals.

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“…The yeast assay was performed mainly following the methods of Routledge and Sumpter (1996) and Wu et al (2002). The yeast strain was grown at 30 • C, 130 rpm overnight, using CuSO 4 supplemented selective medium (SC medium).…”

Section: Recombinant Yeast Assaymentioning

confidence: 99%

Evaluation of chronic toxicities from sediments before and after ecological project in Meiliang Bay, Lake Taihu, using cell bioassays

Qiao

Huang

Wang

2009

Ecological Engineering

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The Emission Soot of Biomass Fuels Combustion as a Source of Endocrine Disrupters

Cited by 18 publications

References 26 publications

In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water of different treatment processes

In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water of different treatment processes

Assessing the Detoxication Efficiencies of Wastewater Treatment Processes Using a Battery of Bioassays/Biomarkers

Evaluation of chronic toxicities from sediments before and after ecological project in Meiliang Bay, Lake Taihu, using cell bioassays

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