The EMT-induced lncRNA NR2F1-AS1 positively modulates NR2F1 expression and drives gastric cancer via miR-29a-3p/VAMP7 axis

Li, Dandan; Xu, Mengjie; Wang, Zidi; Huang, Pan; Huang, Chen; Chen, Zhe; Tang, Gaijuan; Zhu, Xingji; Cai, Mengyu; Qin, Shanshan

doi:10.1038/s41419-022-04540-2

Cited by 46 publications

(28 citation statements)

References 33 publications

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“…Lnc NR2F1-AS1, an antisense lncRNA of NR2F1, is also known as COUP Transcription Factor I. High level of lnc NR2F1-AS1 has been demonstrated to promote progression of gastric cancer and hepatocellular carcinoma [23,24]. In our current study, lnc NR2F1-AS1 was upregulated in BC tissues and cells, and high lnc NR2F1-AS1 expression was closely related to poor prognosis of BC patients.…”

Section: Discussionsupporting

confidence: 48%

Lnc NR2F1-AS1 promotes breast cancer metastasis by targeting miR-25-3p/ZEB2 axis

Zhou,

Zhai,

Kuang

et al. 2022

Preprint

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Background: Long noncoding RNAs (LncRNAs) has been demonstrated to have a considerable effect on tumor metastasis and are aberrently expressed in a variety of cancers. Nevertheless, its role in breast cancer (BC) remains incompletely inventoried.Methods: The microarray assay of differentially expressed lncRNAs in epithelial-mesenchymal transition (EMT) and non-EMT cells was performed. The prognostic value of lnc NR2F1-AS1 expression in BC patients were analyzed in the TCGA database. Lnc NR2F1-AS1 expression level in different BC cell line were assessed by qPCR. The role of lnc NR2F1-AS1 in BC cell metastasis was investigated in vitro and in vivo. Dual luciferase reporter assay and RNA immunoprecipitation (RIP) were performed to uncover the relationship between lnc NR2F1-AS1, miR-25-3p, and ZEB2. Results: High level of lnc NR2F1-AS1 was observed in BC cells undergoing EMT and was clolsely correlated with adverse prognosis in BC patients. Lnc NR2F1-AS1 knockdown notably inhibited BC cell migration, invasiveness in vitro and metastasis in vivo. Mechanistically, lnc NR2F1-AS1 competitively bind with miR-25-3p to impede the degradation of ZEB2, which is a positive EMT transcription factor in BC. Conclusions: Taken together, our study reveals a novel lnc NR2F1-AS1/miR-25-3p/ZEB2 axis for BC metastasis and indicate that lnc NR2F1-AS1 may serve as a potential therapeutic target for breast cancer metastasis.

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Section: Discussionsupporting

confidence: 48%

Lnc NR2F1-AS1 promotes breast cancer metastasis by targeting miR-25-3p/ZEB2 axis

Zhou,

Zhai,

Kuang

et al. 2022

Preprint

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“…In the tumor cells, the dysregulation of ceRNA will lead to the amplified dysregulation of downstream regulators and target molecules. Finally, this cascaded dysregulation of ceRNA network strongly promotes the malignant progression of malignant tumors [ 6 , 7 ].…”

Section: Exosomal Cerna Composition and Biological Functionsmentioning

confidence: 99%

Role of exosomal competitive endogenous RNA (ceRNA) in diagnosis and treatment of malignant tumors

et al. 2022

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Malignant tumors are a threat to human health, thus it is critical to better understand the mechanism of tumor occurrence and development and to find key therapeutic targets. Competitive endogenous RNA (ceRNA) is a type of RNA molecule that includes mRNA of coding-protein, pseudogenes, long non-coding RNA (lncRNA), and circular RNA (circRNA) etc. It is created through a competitive combination of common small RNA (miRNA) and has an inhibitory effect on mRNA translation. ceRNA regulate the post transcriptional expression of genes by competitively binding to common microRNAs (miRNAs).Studies have shown that cernas are involved in tumor cell proliferation, invasion and migration, drug resistance, angiogenesis, as well as tumor immunity, and so on, affecting the progression of tumor development. This article reviews the reported roles of exosomal ceRNA in the diagnosis and treatment of malignant tumors and the mechanisms underlying these.

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“…As pointed in the sections above, VAMP7 acts as a central v-SNARE in regulating unconventional secretion. In specific relation to the above-mentioned molecular and cellular mechanisms of UPS, recent genetic studies particularly using transcriptomics have linked VAMP7 expression to several cancers ( Zhu et al, 2020 ; Wang et al, 2021 ; Xu et al, 2021 ; Li et al, 2022 ). Furthermore, VAMP7 mediates the exosomal release of miR-375 ( Kumar et al, 2021 ), which was involved in glioblastoma and matrix metalloproteases ( Steffen et al, 2008 ).…”

Section: Role Of Unconventional Secretion In Health and Diseasementioning

confidence: 99%

Role of SNAREs in Unconventional Secretion—Focus on the VAMP7-Dependent Secretion

Vats

Galli

2022

Front. Cell Dev. Biol.

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Intracellular membrane protein trafficking is crucial for both normal cellular physiology and cell-cell communication. The conventional secretory route follows transport from the Endoplasmic reticulum (ER) to the plasma membrane via the Golgi apparatus. Alternative modes of secretion which can bypass the need for passage through the Golgi apparatus have been collectively termed as Unconventional protein secretion (UPS). UPS can comprise of cargo without a signal peptide or proteins which escape the Golgi in spite of entering the ER. UPS has been classified further depending on the mode of transport. Type I and Type II unconventional secretion are non-vesicular and non-SNARE protein dependent whereas Type III and Type IV dependent on vesicles and on SNARE proteins. In this review, we focus on the Type III UPS which involves the import of cytoplasmic proteins in membrane carriers of autophagosomal/endosomal origin and release in the extracellular space following SNARE-dependent intracellular membrane fusion. We discuss the role of vesicular SNAREs with a strong focus on VAMP7, a vesicular SNARE involved in exosome, lysosome and autophagy mediated secretion. We further extend our discussion to the role of unconventional secretion in health and disease with emphasis on cancer and neurodegeneration.

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The EMT-induced lncRNA NR2F1-AS1 positively modulates NR2F1 expression and drives gastric cancer via miR-29a-3p/VAMP7 axis

Cited by 46 publications

References 33 publications

Lnc NR2F1-AS1 promotes breast cancer metastasis by targeting miR-25-3p/ZEB2 axis

Lnc NR2F1-AS1 promotes breast cancer metastasis by targeting miR-25-3p/ZEB2 axis

Role of exosomal competitive endogenous RNA (ceRNA) in diagnosis and treatment of malignant tumors

Role of SNAREs in Unconventional Secretion—Focus on the VAMP7-Dependent Secretion

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