The Encapsidated Genome of Microplitis demolitor Bracovirus Integrates into the Host Pseudoplusia includens

Beck, Markus H.; Zhang, Shu; Bitra, Kavita; Burke, Gaelen R.; Strand, Michael R.

doi:10.1128/jvi.05726-11

Cited by 50 publications

(120 citation statements)

References 45 publications

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“…Despite not replicating in lepidopteran host cells, chromosomal integration has been demonstrated both in vitro, in host and non-host derived cell cultures, and in vivo, in parasitized lepidopteran hosts for some PDV circles [70,71]. Viral circle reintegration (of ptp containing circles) in two different genomic locations of Cotesia sesamiae wasps have also been described [64].…”

Section: (D) Bracovirus Segment Reintegration Within Wasp and Lepidopmentioning

confidence: 99%

“…Bracovirus DNA integration into lepidopteran host or secondary reintegration in wasp genomes might involve specific unknown mechanisms. Sequence comparison of circular and reintegrated viral sequences suggested that circle reintegration did not involve DRJs, but was mediated by specific sites in the circles, named left and right junction (figures 3 and 4 and table 2) [64,70]. Furthermore, reintegration also resulted in the deletion of a 40-53 bp viral region (indicated by D in figure 4).…”

Section: (D) Bracovirus Segment Reintegration Within Wasp and Lepidopmentioning

confidence: 99%

“…Similar viral reintegration boundaries have been identified in three BVs (CcBV, GiBV and MdBV), and a particular stretch of viral sequence was lost during the reintegration process (table 2). BV integration is likely more widespread than initially assumed, as for MdBV alone, all 15 circles can persist in cell cultures, and integration motifs could be identified in 12 segments [70]. rstb.royalsocietypublishing.org Phil Trans R Soc B 368: 20130051…”

Section: (D) Bracovirus Segment Reintegration Within Wasp and Lepidopmentioning

confidence: 99%

“…The fact that BV circles can reintegrate into the genomes of both wasp and lepidopteran hosts suggests BVs could mediate horizontal gene transfer between these insects. Initial experimental data indicate reintegrations do happen during natural interactions and could be more frequent than initially expected [64][65][66][67][68][69][70]. For PDV reintegration into wasp DNA, PDV circles would need to enter the wasp germline and to stably integrate into the wasp genome during the phase when parasitoid wasp eggs and larvae are exposed to virus circles in the haemolymph of the lepidopteran host.…”

Section: (D) Bracovirus Segment Reintegration Within Wasp and Lepidopmentioning

confidence: 99%

“…A re-emerging theme in the study of PDV evolution is the ability of viral circles to secondarily integrate into insect (lepidopteran or wasp) host genomic DNA [70]. Despite not replicating in lepidopteran host cells, chromosomal integration has been demonstrated both in vitro, in host and non-host derived cell cultures, and in vivo, in parasitized lepidopteran hosts for some PDV circles [70,71].…”

Section: (D) Bracovirus Segment Reintegration Within Wasp and Lepidopmentioning

confidence: 99%

See 4 more Smart Citations

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

Herniou

Huguet

Thézé

et al. 2013

Phil. Trans. R. Soc. B

157

172

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International audienceThe Polydnaviridae (PDV), including the Bracovirus (BV) and Ichnovirus genera, originated from the integration of unrelated viruses in the genomes of two parasitoid wasp lineages, in a remarkable example of convergent evolution. Functionally active PDVs represent the most compelling evolutionary success among endogenous viral elements (EVEs). BV evolved from the domestication by braconid wasps of a nudivirus 100 Ma. The nudivirus genome has become an EVE involved in BV particle production but is not encapsidated. Instead, BV genomes have co-opted virulence genes, used by the wasps to control the immunity and development of their hosts. Gene transfers and duplications have shaped BV genomes, now encoding hundreds of genes. Phylogenomic studies suggest that BVs contribute largely to wasp diversification and adaptation to their hosts. A genome evolution model explains how multidirectional wasp adaptation to different host species could have fostered PDV genome extension. Integrative studies linking ecological data on the wasp to genomic analyses should provide new insights into the adaptive role of particular BV genes. Forthcoming genomic advances should also indicate if the associations between endoparasitoid wasps and symbiotic viruses evolved because of their particularly intimate interactions with their hosts, or if similar domesticated EVEs could be uncovered in other parasites

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Section: (D) Bracovirus Segment Reintegration Within Wasp and Lepidopmentioning

confidence: 99%

Section: (D) Bracovirus Segment Reintegration Within Wasp and Lepidopmentioning

confidence: 99%

Section: (D) Bracovirus Segment Reintegration Within Wasp and Lepidopmentioning

confidence: 99%

Section: (D) Bracovirus Segment Reintegration Within Wasp and Lepidopmentioning

confidence: 99%

Section: (D) Bracovirus Segment Reintegration Within Wasp and Lepidopmentioning

confidence: 99%

See 3 more Smart Citations

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

Herniou

Huguet

Thézé

et al. 2013

Phil. Trans. R. Soc. B

157

172

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Polydnaviruses: Evolution and Applications

Wei

Pérez-Rodríguez

Robledo-Torres

et al. 2023

Microbial Biodiversity, Biotechnology and Ecosystem Sustainability

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A polydnaviral genome of Microplitis bicoloratus bracovirus and molecular interactions between the host and virus involved in NF-κB signaling

Chen

et al. 2016

Arch Virol

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Polydnaviruses (PDVs) play a critical role in altering host gene expression to induce immunosuppression. However, it remains largely unclear how PDV genes affect host genes. Here, the complete genome sequence of Microplitis bicoloratus bracovirus (MbBV), which is known to be an apoptosis inducer, was determined. The MbBV genome consisted of 17 putative double-stranded DNA circles and 179 fragments with a total size of 336,336 bp and contained 116 open reading frames (ORFs). Based on conserved domains, nine gene families were identified, of which the IκB-like viral ankyrin (vank) family included 28 members and was one of the largest families. Among the 116 ORFs, 13 MbBV genes were expressed in hemocytes undergoing MbBV-induced apoptosis and further analyzed. Three vank genes (vank86, vank92, vank101) were expressed in hemocytes collected from Spodoptera litura larvae parasitized by M. bicoloratus, in which host NF-κB/IκBs, including relish, dorsal, and cactus, were also persistently expressed. When Spli221 cells were infected with MbBV viral particles, mRNA levels of host and viral NF-κB/IκB genes were persistent and also varied in Spli221 cells undergoing virus-induced pre-apoptosis cell from 1 to 5 hours postinfection. Both were then expressed in a time-dependent expression in virus-induced apoptotic cells. These data show that viral IκB-like transcription does not inhibit host NF-κB/IκB expression, suggesting that transcription of these genes might be regulated by different mechanisms.

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The Encapsidated Genome of Microplitis demolitor Bracovirus Integrates into the Host Pseudoplusia includens

Cited by 50 publications

References 45 publications

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

Polydnaviruses: Evolution and Applications

A polydnaviral genome of Microplitis bicoloratus bracovirus and molecular interactions between the host and virus involved in NF-κB signaling

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