The Endocardial Endothelial Na+/K+Atpase and Cardiac Contraction

Fransen, Paul; Hendrickx, Jan; Keulenaer, Gilles W. De

doi:10.1007/978-1-4419-9029-7_4

Cited by 3 publications

(3 citation statements)

References 38 publications

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“…The endocardium is the innermost endothelial layer of the heart, serving as a BHB, an endothelial layer that regulates the ionic composition of the cardiac microenvironment via passive tight junctions and active transporter systems. The endocardium also modulates myocardium performance by releasing trophic factors in response to humoral and mechanical stimuli . In addition to its signaling roles during heart development and regeneration, the endocardium has also been shown to contribute multi‐lineage descendants to cardiac valves, septa, hematopoiesis, and coronary blood vessels .…”

Section: Discussionmentioning

confidence: 99%

Human pluripotent stem cell‐derived epicardial progenitors can differentiate to endocardial‐like endothelial cells

Bao

Bhute

Han

et al. 2017

Bioengineering & Transla Med

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During heart development, epicardial progenitors contribute various cardiac lineages including smooth muscle cells, cardiac fibroblasts, and endothelial cells. However, their specific contribution to the human endothelium has not yet been resolved, at least in part due to the inability to expand and maintain human primary or pluripotent stem cell (hPSC)‐derived epicardial cells. Here we first generated CDH5‐2A‐eGFP knock‐in hPSC lines and differentiated them into self‐renewing WT1+ epicardial cells, which gave rise to endothelial cells upon VEGF treatment in vitro. In addition, we found that the percentage of endothelial cells correlated with WT1 expression in a WT1‐2A‐eGFP reporter line. The resulting endothelial cells displayed many endocardium‐like endothelial cell properties, including high expression levels of endocardial‐specific markers, nutrient transporters and well‐organized tight junctions. These findings suggest that human epicardial progenitors may have the capacity to form endocardial endothelium during development and have implications for heart regeneration and cardiac tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Human pluripotent stem cell‐derived epicardial progenitors can differentiate to endocardial‐like endothelial cells

Bao

Bhute

Han

et al. 2017

Bioengineering & Transla Med

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“…Two possible signaling mechanisms from the cardiac endothelium to cardiomyocytes have been proposed: (1) a paracrine pathway, described as “stimulus-secretion-contraction coupling”, and (2) a transendothelial ion transport, described as “ionic blood-heart barrier” (Fransen et al 2001, 2003). The cardiac inotropic effect induced by endothelial paracrine factors is associated with a reduction in a myofilament responsiveness to Ca 2+ rather than an alteration in Ca 2+ transients (Shah et al 1996).…”

Section: Cardiac Endotheliummentioning

confidence: 99%

Endothelium-Driven Myocardial Growth or Nitric Oxide at the Crossroads

Tı̂rziu

Simons

2008

Trends in Cardiovascular Medicine

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Endothelium lining the coronary vasculature and the heart chambers is a dynamic sensor that serves a variety of functions including bi-directional communications with cardiac myocytes. Among endothelium-released factors nitric oxide exerts multifactorial effects on various cell types in the heart and may play a role in growth of the vasculature and myocardial hypertrophy. This review summarizes new data regarding the endothelium-to-myocyte signaling focusing on its role in regulation of cardiac hypertrophy through a nitric oxide mediated paracrine signal.

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“…In the present study, similar to the ERα mutation in mice, low convolution and less branching were observed in the cyp19a1b −/− mutant testes, which might have caused a reduction in the epithelial area involved in ion exchange. In addition, blood is essential for ion exchange (Fransen, Hendrickx, & De Keulenaer, ; Fransen, Hendrickx, Brutsaert, & Sys, ; Josh Yeh et al, ). The absence of blood vessels inside the efferent duct wall of the mutant testes may have also had a negative effect on the ion exchange.…”

Section: Discussionmentioning

confidence: 99%

Mutation ofcyp19a1bresults in sterile males due to efferent duct obstruction in Nile tilapia

Zhang

Min

et al. 2019

Molecular Reproduction Devel

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The involvement of estrogen in male fertility has been well established in mammals. However, less is known about the role of estrogen in fish male reproduction. Our recent study revealed that Cyp19a1a deficiency had no effect on fertility in male fish. In this study, expression of Cyp19a1b, but not Cyp19a1a, was detected by immunohistochemistry in Leydig cells of tilapia testes. cyp19a1b mutation resulted in a significant decrease in the concentration of 17β‐estradiol in serum and sterility in XY fish, as no offspring were obtained when crossed with control XX fish at 240 days after hatching (dah). No sperm was obtained from the mature mutants by in vitro extrusion. Further examination of the mutant gonads revealed excessive semen accumulation and testicular hypertrophy. Semen collected from the mutant testes during autopsy contained sperm with a normal morphology that showed no significant differences in motility, VCL, BCF, STR, or fertility compared with control sperm. Efferent ducts from the mutant testes, which had low‐convolution levels, fewer branches, and no blood vessels observed inside the walls, were significantly smaller in size. qRT‐PCR analyses showed downregulated expression of ion exchange genes. There was increased apoptosis in the epithelial cells of the efferent ducts and other somatic cells of the testes as revealed by TUNEL staining, as well as upregulation of apoptosis gene expression in the mutants. At 360 dah, mutant fish showed testicular atrophy and efferent duct fibrosis. These results demonstrated that estrogen deficiency caused by Cyp19a1b mutation resulted in male sterility due to efferent duct obstruction.

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The Endocardial Endothelial Na+/K+Atpase and Cardiac Contraction

Cited by 3 publications

References 38 publications

Human pluripotent stem cell‐derived epicardial progenitors can differentiate to endocardial‐like endothelial cells

Human pluripotent stem cell‐derived epicardial progenitors can differentiate to endocardial‐like endothelial cells

Endothelium-Driven Myocardial Growth or Nitric Oxide at the Crossroads

Mutation ofcyp19a1bresults in sterile males due to efferent duct obstruction in Nile tilapia

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