2022
DOI: 10.1038/s41477-022-01280-1
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The endocytic TPLATE complex internalizes ubiquitinated plasma membrane cargo

Abstract: Endocytosis controls the perception of stimuli by modulating protein abundance at the plasma membrane. In plants, clathrin-mediated endocytosis is the most prominent internalization pathway and relies on two multimeric adaptor complexes, the AP-2 and the TPLATE complex (TPC). Ubiquitination is a well-established modification triggering endocytosis of cargo proteins, but how this modification is recognized to initiate the endocytic event remains elusive.Here, we show that TASH3, one of the large subunits of TPC… Show more

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Cited by 14 publications
(19 citation statements)
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“…TPC may also be involved in the regulation of H + -fluxes across the plasma membrane. A mutant harboring defects in the large TPLATE subunit TASH3 – referred to as nosh (no SH3) - showed reduced internalization of TRANSMEMBRANE KINASE 1 (TMK1) (81). TMK1 is required for auxin-induced activation of plasma membrane H + -ATPases via phosphorylation, promoting cell wall acidification and cell elongation (82).…”
Section: Discussionmentioning
confidence: 99%
“…TPC may also be involved in the regulation of H + -fluxes across the plasma membrane. A mutant harboring defects in the large TPLATE subunit TASH3 – referred to as nosh (no SH3) - showed reduced internalization of TRANSMEMBRANE KINASE 1 (TMK1) (81). TMK1 is required for auxin-induced activation of plasma membrane H + -ATPases via phosphorylation, promoting cell wall acidification and cell elongation (82).…”
Section: Discussionmentioning
confidence: 99%
“…We also identified a link between AtEH1 and TOM1-LIKE (TOL) proteins, which function in plants as ubiquitin receptors, analogous to the ESCRT-0 complex (Korbei et al, 2013; Mosesso et al, 2019; Moulinier-Anzola et al, 2020). We speculate that recognition of TOL proteins by AtEH subunits promote condensation and endocytosis initiation at membrane regions enriched for ubiquitinated cargo, possibly in co-operation with the TPC subunit TASH3 (Grones et al, 2022). This coupling of TPC recruitment and condensation to the co-operative recognition of lipids and ubiquitinated cargo would ensure the highly regulated assembly of clathrin-coated vesicles on the plasma membrane.…”
Section: Discussionmentioning
confidence: 99%
“…Images were acquired using the Leica SP8X system using a ×40 W 1.2 NA objective using 488 nm laser excitation, and collection from 650nm - 750nm. The ratio of plasma membrane signal and cytosolic signal were calculated using an automated detection macro in ImageJ ‘detect_membrane_and_cytoplasm_from_a_roi.ijm’ (Grones et al, 2022), using a threshold set to 60.…”
Section: Methodsmentioning
confidence: 99%
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