With Two Text-figures.) INVESTIGATIONS concerning the protein nutrition of poultry appear mainly to have taken the form of feeding experiments in which growth or egg production have been compared on diets differing in the quality and quantity of protein which they contain. In view of the importance of protein in poultry nutrition, it is surprising that few accurately controlled nitrogen balance experiments are reported in the literature.Ackerson, Blish and Mussehl(i,2) in America have measured the •endogenous loss of nitrogen by poultry by means of the balance experiment, and in this country Hainan (3) has reported the results of balance experiments carried out with laying and non-laying pullets. Hainan determined weekly nitrogen, calcium and phosphorus balances with two birds whose food intake was controlled. He found that both birds remained in positive nitrogen balance during the greater part of the fourteen weeks of the experiment, serious negative balances occurring only during the latter weeks when the birds were laying. His results indicate a marked storage of nitrogen during the week prior to the commencement of laying, this storage being maintained during the early ••stages of laying and falling off towards the end of the laying period. Hainan concluded that preliminary storage of nitrogen for egg production does not extend beyond a week or so prior to the commencement of •egg laying, and that the nitrogen required for the purpose can be largely if not entirely supplied from the food given during the egg laying period.The experiment here to be described was undertaken with a view to studying any variation which might occur in the daily retention of nitrogen by laying birds and in order to arrive at an estimate of the nitrogen requirement for egg production.EXPERIMENTAL.
The birds used.Two Rhode Island Red hens, which were in full lay, were selected for the purpose of the experiment. These birds had been reared on free range and subsequently kept semi-intensively on a good laying ration. 1 The cost of this investigation was met from a grant made to the University of Leeds by Sir Philip Eeokitt.