The Endophytic Fungus Chaetomium cupreum Regulates Expression of Genes Involved in the Tolerance to Metals and Plant Growth Promotion in Eucalyptus globulus Roots

Ortiz, Javier; Soto, Javiera; Fuentes, Alejandra; Herrera, Héctor; Meneses, Claudio; Arriagada, César

doi:10.3390/microorganisms7110490

Cited by 37 publications

(19 citation statements)

References 52 publications

(58 reference statements)

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“…The dual inoculation also affects the root exudates, which maybe directly related with improved plant growth [55]. In our study, malic and citric acids were observed as the main root exudates from plants under PAH stress, which agrees with recent studies analyzing the concentration of root exudates of native plants subjected to different abiotic stresses [79,80]. Furthermore, malic and citric acid can mobilize inorganic P in soil, promoting plant growth [81].…”

Section: Discussionsupporting

confidence: 90%

Inoculation of Triticum Aestivum L. (Poaceae) with Plant-Growth-Promoting Fungi Alleviates Plant Oxidative Stress and Enhances Phenanthrene Dissipation in Soil

Lagos

Larsen²,

Fuentes

et al. 2021

Agronomy

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Polycyclic aromatic hydrocarbons (PAHs) are strong toxic compounds mainly released to the environment during combustion of fossil fuels, and have strong toxic effects on living organisms, with soil being one of their main reservoirs. High PAH levels in soils can interfere with plant growth and biomass production, causing several losses of diversity. In this study, we evaluated the effects of the co-inoculation of Trichoderma viride and Funneliformis mosseae on PAH dissipation and alleviation of oxidative stress in Triticum aestivum L. (wheat) plants growing in a phenanthrene-spiked soil. We determined the effect of single and dual fungal inoculation on phenanthrene dissipation rates, soil enzyme activities, dry biomass, antioxidant enzymes, lipid peroxidation, and organic acid exudation of plants growing in a soil spiked with phenanthrene at 500 and 1000 mg kg−1 soil. The co-inoculation with T. viride and F. mosseae resulted in a high phenanthrene dissipation from the soil. Also, dry biomass, soil enzymes, antioxidant response, organic acid exudation and phenanthrene content in roots were increased by the dual inoculation treatments, whereas lipid peroxidation and phenanthrene content in shoots were reduced. Our results show that the co-inoculation with these two soil fungi significantly promotes phenanthrene dissipation from soil and contributes to alleviating oxidative damage in wheat plants exposed to high levels of phenanthrene.

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Section: Discussionsupporting

confidence: 90%

Inoculation of Triticum Aestivum L. (Poaceae) with Plant-Growth-Promoting Fungi Alleviates Plant Oxidative Stress and Enhances Phenanthrene Dissipation in Soil

Lagos

Larsen²,

Fuentes

et al. 2021

Agronomy

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“…It was found that Piriformospora indica, an endophytic fungus, promotes the growth of Arabidopsis and tobacco seedlings and enhances the expression of genes encoding nitrate reductase [51]. It was also noted that the endophytic fungus C. cupreum positively regulates the expression of some genes involved in auxin biosynthesis and metabolism in Eucalyptus globulus [52]. The modification of tomato root architecture is attributed to ethylene/indole-3-acetic acid signaling triggered by a strain of Trichoderma [53].…”

Section: Discussionmentioning

confidence: 99%

Harnessing the Rhizosphere of the Halophyte Grass Aeluropus littoralis for Halophilic Plant-Growth-Promoting Fungi and Evaluation of Their Biostimulant Activities

Tarroum

Romdhane

Ali

et al. 2021

Plants

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Hydroponic systems have gained interest and are increasingly used in hot and dry desert areas. Numbers of benefits are offered by hydroponic systems such as the ability to save water, enhance nutrients use efficiency, easy environmental control, and prevention of soil-borne diseases. However, the high consumption of chemical fertilizers for nutrient solution and the sensitivity of closed hydroponic systems to salinity are issues that need solutions. Thus, the main goal of our research activities is to isolate plant growth promoting fungi in order to develop sustainable hydroponic systems. We are working on isolating and testing the possibility to incorporate the cell-free filtrate (CFF) of plant growth promoting fungi (PGPF) in the composition of the nutrient solution. In this work, we isolated six strains of PGPF from the rhizosphere of the halophyte grass Aeluropus littoralis. Phylogenetic analyses of DNA sequences amplified by ITS1 and ITS4 primers identified the isolated fungi as: Byssochlamys spectabilis, Chaetomium globosum, Cephalotheca foveolata, Penicillium melinii, Alternaria tenuissima, and Nigrospora chinensis. The promoting of vigor in tobacco seedlings was used as criteria to evaluate the biostimulant activity of these fungi by adding either their mycelia (DE: direct effect) or their cell-free filtrates (CFF: indirect effect) to the plant-growth media. The best significant growth stimulation was obtained with plants treated by B. spectabilis. However, only the CFFs of Byssochlamys spectabilis (A5.1) and Penicillium melinii (A8) when added at a dilution factor of 1/50 to half-strength nutritive solution (0.5NS) resulted in significant improvement of all assessed growth parameters. Indeed, the A5.1CFF and A8CFF in 0.5NS induced a significant better increase in the biomass production when compared to NS or 0.5NS alone. All fungi produced indole acetic acid in the CFFs, which could be one of the key factors explaining their biostimulant activities. Furthermore, six genes involved in nitrogen-metabolism (NR1 and NRT1), auxin biosynthesis (Tryp1 and YUCCA6-like), and brassinosteroid biosynthesis (DET2 and DWF4) were shown to be induced in roots or leaves following treatment of plants with the all CFFs. This work opens up a prospect to study in deep the biostimulant activity of PGPFs and their applications to decrease the requirement of chemical fertilizers in the hydroponic growing systems.

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“…It is expected that effective tolerance mechanisms can support colonization of acidic Andisols. One of the tolerance mechanisms can be related with speci c interactions with microbial strains which can confer metal tolerance to their associated plants (Ortiz et al, 2019). This is the case of Rhodococcus sp., which has been described as a microbial strain with high tolerance to metal(loid)s (Kumari et al, 2019;Vergani et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Early Growth Stage Root-Associated Endophytes Isolated From Ulex Europaeus L. (Fabaceae) Colonizing Rural Areas in South-Central Chile

Herrera¹,

Fuentes²,

Ortiz³

et al. 2021

Preprint

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Background and aimsUlex europaeus L. (Fabaceae), commonly known as gorse, is an invasive woody shrub that easily grows in several locations across the world. However, little is known about the interactions of this invasive species with soil microorganisms and how these microbes can promote reaching rapid grow-rates at early stages of development. We aim at characterizing the endophytic fungal and bacterial microbiota associated with roots of early growth stage U. europaeus colonizing native ecosystems in south-central Chile.MethodsRoot-associated microorganisms were isolated and identified using standard molecular techniques. Furthermore, plant growth-promoting traits were studied and biocontrol activity was assessed to characterize the early growth stage root-associated taxa. ResultsFour endophytic fungi belonging to Sordariomycetes and twelve bacteria assigned to Proteobacteria and Actinobacteria were identified as the principal early growth stage root-endophytic taxa. Plant growth-promoting traits were detected in several isolates such as Fusarium acuminatum and Rhodococcus sp. Besides, some of the isolates such as Rhodococcus sp. and Purpureocillium lilacinum showed biocontrol potential against phytopathogenic fungi. ConclusionsOur results demonstrate that early growth stage root endophytic taxa associated with U. europaeus have beneficial plant growth-promoting traits that can contribute with the rapid growth-rates of the shrub. The interaction with a set of beneficial microorganisms is an additional mechanism to explain the ability of U. europaeus for colonizing in various ecosystems.

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The Endophytic Fungus Chaetomium cupreum Regulates Expression of Genes Involved in the Tolerance to Metals and Plant Growth Promotion in Eucalyptus globulus Roots

Cited by 37 publications

References 52 publications

Inoculation of Triticum Aestivum L. (Poaceae) with Plant-Growth-Promoting Fungi Alleviates Plant Oxidative Stress and Enhances Phenanthrene Dissipation in Soil

Inoculation of Triticum Aestivum L. (Poaceae) with Plant-Growth-Promoting Fungi Alleviates Plant Oxidative Stress and Enhances Phenanthrene Dissipation in Soil

Harnessing the Rhizosphere of the Halophyte Grass Aeluropus littoralis for Halophilic Plant-Growth-Promoting Fungi and Evaluation of Their Biostimulant Activities

Early Growth Stage Root-Associated Endophytes Isolated From Ulex Europaeus L. (Fabaceae) Colonizing Rural Areas in South-Central Chile

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