The endothelial status reflected by circulating endothelial cells, circulating endothelial progenitor cells and soluble thrombomodulin in patients with mild and resistant hypertension

Budzyń, Magdalena; Gryszczyńska, Bogna; Boruczkowski, Maciej; Kaczmarek, Mariusz; Begier-Krasińska, Beata; Osińska, Angelika; Bukowska, Alicja; Iskra, Maria; Kasprzak, Magdalena Paulina

doi:10.1016/j.vph.2018.12.005

Cited by 19 publications

(21 citation statements)

References 63 publications

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“…In the present study, a significant and positive correlation was found between sRAGE and number of CEPCs in the MH group, which led us to hypothesize that an intensified formation of sRAGE as well as increased numbers of CEPCs confirms the protective mechanisms against progressive endothelium damage. However, in our previous study, we also noticed that the process of endothelial regeneration is inadequately reflected by a significantly lower CEPC/CEC ratio in both MH and RH patients versus C group [23]. We demonstrated that the AGEs/sRAGE ratio is negatively correlated with the number of CEPCs in the MH group.…”

Section: Discussionmentioning

confidence: 55%

“…noted that sRAGE may play a role in arterial stiffening a crucial complication associated with hypertension in patients [25]. In our previous study, we demonstrated an increased CEC numbers and a decreased CEPC/CEC ratio in both MH and RH patients in comparison with normotensive volunteers [23]. Furthermore, a tendency of CEPC numbers to rise in both types of hypertensive patients was observed.…”

Section: Discussionmentioning

confidence: 71%

“…The first assumes that oxidative stress associated with patients in the MH group is more intense, despite significantly lower SBP1 and fewer complications than is characteristic of RH patients. In the previous study, we demonstrated that the oxidative stress plays a crucial role in the pathogenesis of chronic venous insufficiency and, importantly, occurs in early stage of disease development [23]. This interesting concept indicates a possible adaptive mechanism by which inhibition of further oxidation may have taken place in RH patients.…”

Section: Discussionmentioning

confidence: 89%

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Association between Advanced Glycation End Products, Soluble RAGE Receptor, and Endothelium Dysfunction, Evaluated by Circulating Endothelial Cells and Endothelial Progenitor Cells in Patients with Mild and Resistant Hypertension

Gryszczyńska

Budzyń

Begier-Krasińska

et al. 2019

IJMS

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The aim of the present study was to evaluate advanced glycation end products (AGEs) and soluble form of receptor RAGE (sRAGE) concentrations as well as the AGEs/sRAGE ratio in mild (MH) and resistant (RH) hypertensive patients in comparison with normotensive individuals. We also evaluated the association between AGEs, sRAGE as well as AGEs/sRAGE ratio and circulating endothelial cells (CECs) and circulating endothelial progenitor cells (CEPCs). The MH group consisted of 30 patients, whereas 30 patients were classified for the RH group. The control group (C) included 25 normotensive volunteers. AGEs and sRAGE were measured using enzyme-linked-immunosorbent assay (ELISA). The multicolor flow cytometry was used for analysis of CECs and CEPCs. Significantly higher levels of AGEs in RH cohort were observed as compared to C cohort. Furthermore, significantly lower sRAGE levels as well as a higher AGEs/sRAGE ratio were observed between MH and RH cohorts. Significant correlations were found in the MH cohort for sRAGE and CECs, and CEPCs. The elevation of AGEs levels suggests that oxidative modification of proteins occurs in hypertension pathogenesis. The decrease in sRAGE levels and elevation of the AGEs/sRAGE ratio in MH and RH groups may suggest that hypertensive patients are less protected against the side effects of AGEs as a consequence of an insufficient competitive role of sRAGE against the AGEs-RAGE axis. Finally, it may be concluded that the level of AGEs may be an independent predictor of the condition and function of the endothelium. Furthermore, sRAGE may be classified as a potential biomarker of inflammation and endothelium dysfunction.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 89%

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Association between Advanced Glycation End Products, Soluble RAGE Receptor, and Endothelium Dysfunction, Evaluated by Circulating Endothelial Cells and Endothelial Progenitor Cells in Patients with Mild and Resistant Hypertension

Gryszczyńska

Budzyń

Begier-Krasińska

et al. 2019

IJMS

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“…In our previous work, we demonstrated a higher number of CECs and a drastically lowered CEPCs/CECs ratio in patients with mild (MH) and RH (Budzyń et al, 2018). In the present study, for the first time, we tried to determine the potential of these cells in the prediction of LVH in the same group of hypertensive patients.…”

Section: Introductionmentioning

confidence: 82%

“…Blood was collected from each patient into ethylenediaminetetraacetic acid (EDTA) anticoagulant and used for flow cytometric analysis. Multicolor flow cytometry analysis was performed according to the method published by Szpera-Goździewicz et al (2017) and modified by us elsewhere (Budzyń et al, 2018). The conjugated mouse anti-human monoclonal antibodies: CD34, CD146, CD45, and CD133 (BioLegend, London, United Kingdom) were used.…”

Section: Methodsmentioning

confidence: 99%

The Potential Role of Circulating Endothelial Cells and Endothelial Progenitor Cells in the Prediction of Left Ventricular Hypertrophy in Hypertensive Patients

et al. 2019

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Background The main aim of present study is to evaluate the potential role of circulating endothelial cells (CECs) and endothelial progenitor cells (CEPCs) – representing specific markers of endothelial damage, in the prediction of left ventricular hypertrophy (LVH) in hypertensive patients categorized into two groups; mild (MH) and resistant hypertension (RH). Materials and Methods Thirty patients with MH and 28 subjects with RH were involved in the study. In both groups, patients were divided into an LVH and non-LVH group. The control group included 33 age and sex-matched normotensive volunteers. Physical examination, laboratory tests and echocardiography were conducted. Results In both the MH and RH group, patients with as well as without LVH demonstrated a higher number of CECs and a lower ratio of CEPCs/CECs as compared to the healthy control. Multiple linear regression analysis showed a positive association of CEPCs with left ventricular mass (LVM) and left ventricular mass index (LVMI), independently of other confounders. Conclusion Our results suggest that endothelial injury observed as an elevated CECs number and its impaired regeneration, reflected by a lowered CEPCs/CECs ratio, precede LVH occurrence and may play a significant role in LVH development regardless of the clinical severity of hypertension. Moreover, independent correlation of CEPCs with echocardiographic (ECG) incidences of LVH suggests their potential use as a screening biomarker to stratify the risk of LVH development.

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Advances on the Experimental Research in Resistant Hypertension

Irigoyen,

Fetter,

De Angelis

2024

Curr Hypertens Rep

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The endothelial status reflected by circulating endothelial cells, circulating endothelial progenitor cells and soluble thrombomodulin in patients with mild and resistant hypertension

Cited by 19 publications

References 63 publications

Association between Advanced Glycation End Products, Soluble RAGE Receptor, and Endothelium Dysfunction, Evaluated by Circulating Endothelial Cells and Endothelial Progenitor Cells in Patients with Mild and Resistant Hypertension

Association between Advanced Glycation End Products, Soluble RAGE Receptor, and Endothelium Dysfunction, Evaluated by Circulating Endothelial Cells and Endothelial Progenitor Cells in Patients with Mild and Resistant Hypertension

The Potential Role of Circulating Endothelial Cells and Endothelial Progenitor Cells in the Prediction of Left Ventricular Hypertrophy in Hypertensive Patients

Advances on the Experimental Research in Resistant Hypertension

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