The Energy Function of Rat Cardiac Mitochondria Under Artificial Hypobiosis [

Melnytchuk, S.D.; Khyzhnyak, S. V.; Morozova, Valeriia; Stepanova, L. I.; Umanskaya, A.A.; Voitsitsky, V.

doi:10.15407/fz61.02.015

Cited by 3 publications

(4 citation statements)

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“…The obtained results indicate that in hypothermia development under hypoxic-hypercapnic influence, the IMM reorganizations are specific for cardiomyocytes and hepatocytes, and are likely to have compensatory nature, directed at maintaining of functional activity of mitochondrial membranes, especially of the respiratory chain under new conditions of existence, as it was shown previously [9,21].…”

Section: T a B L E 2 The Content Of Fatty Acids (Fas) Of Inner Mitocsupporting

confidence: 69%

“…This condition was created according to Bakhmetiev-Dzay-Anzhus, combining hypoxic-hypercapnic influence with external cooling as described in detail [9].…”

Section: Methodsmentioning

confidence: 99%

“…Regarding the course of oxidative processes in IMM, modification of functional activity of respiratory chain complexes during hypothermia under the influence of hypoxia-hypercapnia [9,21] may increase the formation of ROS in the membrane. According to current views, oleic acid is not only a structural component of cell membranes, but also the main endogenous ROS acceptor and has a high rate of ATP oxidation if compared with other of polyenoic UFAs (arachidonic and linoleic).…”

Section: T a B L E 2 The Content Of Fatty Acids (Fas) Of Inner Mitocmentioning

confidence: 99%

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Fatty acids composition of inner mitochondrial membrane of rat cardiomyocytes and hepatocytes during hypoxia-hypercapnia

Khyzhnyak¹,

Мидык²,

Sysoliatin³

et al. 2016

Ukr.Biochem.J.

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We studied the influence of hypoxic-hypercapnic environment under the effect of hypothermia (artificial hibernation) C hemical composition of phospholipids that are structural components of cell membranes plays an important role in their functioning and various processes in cells. In particular, saturated fatty acids (SFAs) are the main energy substrate for cardiomyocytes. Unsaturated fatty acids (UFAs), due to their ability to increase the degree of unsaturation in phospholipid acyl chains and reduce microviscosity of cellular membranes, are very important in the regulation of membrane permeability and functioning of membrane-bound proteins. In addition, certain UFAs are precursors of physiologically active substances, such as eicosanoids [1].Modification of lipid composition, affecting the intensity of metabolism, acts as a compensatory mechanism that provides functionality of membrane under various conditions. Particularly, changes in ambient temperature, hypoxia, etc. lead to certain shifts in the composition of eukaryotic membrane lipids [2,3]. The study of animal adaptations to different environmental conditions remains current issue of theoretical and practical biology.Non-hibernating mammals influenced by hypothermia in hypoxic-hypercapnic gaseous medium fall into so-called cold narcosis or artificial hibernation state. This results in reduction of metabolic rate along with changes in bioenergetic processes in tissues and mitochondria [4,5].The main suppliers of energy stored in the form of ATP in eukaryotic cells are mitochondria. Their functional activity is provided by the inner membrane which contains components of the electron transport chain and ATP synthase that are needed to transform the energy of electrons transfer for ATP synthesis. This energy is crucial for specific cellular functions, including response formation to external stimuli [6]. The matter of particular interest is the study of the role of lipids in mitochondrial membranes adaptation to extreme conditions. Due to the changes in concentration and ratio of fatty acids, lipid composition of membranes undergoes reorganization, creating optimal conditions for preserving functional activity of intracellular organelles in particular and cells in general [7].The studies of temperature adaptation of homeotherms indicate the regulatory role of lipids in hibernation. However, natural and artificial hibernation affect the lipid composition of cell membranes of mammals in different ways [8]. In addition, existing data on the effects of low temperatures or artificial hibernation on the chemical composition of lipids in mammals tissues are uncertain.

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Section: T a B L E 2 The Content Of Fatty Acids (Fas) Of Inner Mitocsupporting

confidence: 69%

“…This condition was created according to Bakhmetiev-Dzay-Anzhus, combining hypoxic-hypercapnic influence with external cooling as described in detail [9].…”

Section: Methodsmentioning

confidence: 99%

Section: T a B L E 2 The Content Of Fatty Acids (Fas) Of Inner Mitocmentioning

confidence: 99%

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Fatty acids composition of inner mitochondrial membrane of rat cardiomyocytes and hepatocytes during hypoxia-hypercapnia

Khyzhnyak¹,

Мидык²,

Sysoliatin³

et al. 2016

Ukr.Biochem.J.

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“…Вплив гострої гіпоксії-гіперкапнії при зниженні температури тіла створювали за методикою Бахметьєва -Джайя -Анжуса, як детально описано в попередніх роботах [2,7]. Тварин поміщали в герметично закриту камеру, об'єм якої становив 3 дм 3 , за температури довкілля 3-4ºС.…”

Section: методикаunclassified

Аntioxidant Defense System State in the Liver and the Myocardium of Rats Under Conditions of Acute Hypoxia-Hypercapnia

Khyzhnyak¹,

Morozova²,

Мидык³

et al. 2019

Fiziol Zh

Self Cite

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Проведена оцінка антиоксидантного статусу тканин щура за умов гострої гіпоксії-гіперкапнії при зниженні температури тіла. Встановлене зниження вмісту активних продуктів 2-тіобарбітурової кислоти (ТБК-АП) в печінці та міокарді на 48,2 та 51,0% відповідно свідчить про пригнічення окисних процесів у тканинах. При цьому в печінці активність супероксиддисмутази (СОД) знижувалася на 24,2%, переважно за рахунок молекулярної форми Сu,Zn-СОД, а каталази зростала на 29,4%. У міокарді активність СОД зростала на 62,7 %, переважно за рахунок молекулярної форми Mn-СОД, а каталази зменшувалася на 21,9%. Досліджувані показники відновлювалися до контрольних значень через 24 год після зняття впливу гіпоксії-гіперкапнії. Показано різноспрямовані зміни активності глутатіонпероксидази та вмісту відновленого глутатіону в тканинах щурів в умовах досліду. Зроблено висновок, що у разі гострої гіпоксії-гіперкапнії перебіг окисних процесів у організмі щурів знаходиться під контролем системи антиоксидантного захисту. Обговорюється можлива регуляторна роль гіперкапнії.

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State of Hypobiosis During E.COLI Infection: A Time Framework of Animal Survival and Success of Method Application in Conditions of Early and Later Diagnosis of Diseases

Tsvilikhovskyi

Tekdemir

Makhendirarasa

2020

VPBM

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The Energy Function of Rat Cardiac Mitochondria Under Artificial Hypobiosis [

Cited by 3 publications

References 5 publications

Fatty acids composition of inner mitochondrial membrane of rat cardiomyocytes and hepatocytes during hypoxia-hypercapnia

Fatty acids composition of inner mitochondrial membrane of rat cardiomyocytes and hepatocytes during hypoxia-hypercapnia

Аntioxidant Defense System State in the Liver and the Myocardium of Rats Under Conditions of Acute Hypoxia-Hypercapnia

State of Hypobiosis During E.COLI Infection: A Time Framework of Animal Survival and Success of Method Application in Conditions of Early and Later Diagnosis of Diseases

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