Nano silver-based inorganic structure with the chemical formulation of [Ag(dafo) 2 (NO 3)] (1), dafo = 4,5-diazafluoren-9one, was synthesized using sonochemical method. Three different ultrasonic generator output powers of 30 W, 60 W, 90 W, each in two different reaction temperature ranges of 0-10°C and 25-35°C, were our sonochemical reaction conditions. After preparing compound 1 with differently-sized nanoparticles under the sonochemical reaction conditions, we conducted an evaluation study into the optical, anti-cancer, and thermal activities of the compound. High ultrasonic generator output power and low reaction temperature were the two prevailing conditions that affected the size and purity of the nanoparticles of compound 1. Based on our identifying methods, nanoparticles of 90 W at 0-10°C showed the lowest particles size among other nanoparticles. The best optical, anticancer, and thermal activities appertained to the nanoparticles. The nanoparticles were heated to 500°C in an electric furnace to synthesize phase-pure silver nanoparticles.