The enhanced immune response of PCV-2 vaccine using Rehmannia glutinosa polysaccharide liposome as an adjuvant

Huang, Yee; Liu, Zhenguang; Bo, Ruonan; Xing, Jie; Luo, Li; Zhen, Sisi; Niu, Yale; Hu, Yuanliang; Liu, Jiaguo; Wu, Yi; Wang, Deyun

doi:10.1016/j.ijbiomac.2016.02.003

Cited by 24 publications

(11 citation statements)

References 52 publications

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“…As for the changes of PDI, small-scale fluctuations were presented by all the four groups. Besides, RGPL co-encapsulated with PCV-2 antigen also showed similar results, 18 which further validated that the stability RGPL was not antigen specific.…”

Section: Physical Properties Of Rgplmentioning

confidence: 60%

“…18 The lymphocytes prepared from vaccinated mice were re-stimulated with 20 μL of polyhydroxyalkanoate (PHA, 10 μg/mL; Sigma, St. Louis, MO, USA), lipopolysaccharide (LPS; 5 μg/mL; Sigma), or RPMI 1640 medium. After 44 h of incubation at 37°C, 30 μL of MTT solution (5 mg/mL) was added to each well and incubation was continued for an additional 4 h. The supernatant was removed and then the formazan salts formed were dissolved by 100 μL of DMSO.…”

Section: In Vivo Proliferation Of Splenic Lymphocytesmentioning

confidence: 99%

“…18 During the experiments, none of the mice showed any signs of local irritation or systemic discomfort.…”

mentioning

confidence: 95%

“…18 The controlled release of liposome allowed the high level of cytokine and IgG subtype production to be maintained for a prolonged period of time. In addition, results for OVA-specific IgG ( Figure 3A) and PCV-2-specific IgG 18 exhibited that RGPL could better improve the level of antibodies than RGP. In the first week, there was no significant difference among the groups.…”

mentioning

confidence: 99%

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<em>Rehmannia glutinosa</em> polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells

Huang¹,

Qin²,

Huang³

et al. 2016

IJN

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Section: Physical Properties Of Rgplmentioning

confidence: 60%

Section: In Vivo Proliferation Of Splenic Lymphocytesmentioning

confidence: 99%

“…18 During the experiments, none of the mice showed any signs of local irritation or systemic discomfort.…”

mentioning

confidence: 95%

mentioning

confidence: 99%

See 2 more Smart Citations

<em>Rehmannia glutinosa</em> polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells

Huang¹,

Qin²,

Huang³

et al. 2016

IJN

Self Cite

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“…22,23 Vaccination is a highly efficient way of preventing viral and other infectious diseases and remains one of the most effective health care measures introduced into medical practice. 24 Numerous materials have been considered as vaccine adjuvants in the development of modern vaccines, 25 with important roles in drug delivery systems. 26 The vaccine adjuvant activities of several kinds of nanomaterials, including carbon nanotubes, gold nanoparticles, and biodegradable polymeric particles, have been investigated and found to be key ingredients for improving and regulating antigen-specific humoral and cellular immune responses.…”

Section: Introductionmentioning

confidence: 99%

Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres

Zheng

Qin

et al. 2018

IJN

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Background Poly lactide (PLA) was proved in the last years to be good for use in sustained drug delivery and as carriers for vaccine antigens. In our previous research, pachyman (PHY)-encapsulated PLA (PHYP) nanospheres were synthesized and their function of controlling drug release was demonstrated. Purpose In order to modify the fast drug-release rate of PHY when inoculated alone, the maturation of bone marrow dendritic cells (BMDCs) in vitro and their immunological enhancement in vivo were explored using PHYP nanospheres. Methods The maturation and antigen uptake of BMDCs were evaluated, both alone and with formulated antigen PHYP nanospheres, ie, ovalbumin (OVA)-loaded PHYP nanospheres, as an antigen delivery system, to investigate antigen-specific humoral and cellular immune responses. Results The results indicated that, when stimulated by PHYP, the BMDCs matured as a result of upregulated expression of co-stimulatory molecules; the mechanism was elucidated by tracing fluorescently labeled antigens in confocal laser scanning microscopy images and observing the uptake of nanospheres by transmission electron microscopy. It was further revealed that mice inoculated with OVA-PHYP had augmented antigen-specific IgG antibodies, increased cytokine secretion by splenocytes, increased splenocyte proliferation, and activation of cluster of differentiation (CD)4 + and CD8 + T cells in vivo. Elevated immune responses were produced by OVA-PHYP, possibly owing to the activation and maturation of dendritic cells (in draining lymph nodes). Conclusion It was corroborated that PHY- and/or OVA-encapsulated PLA nanospheres elicited prominent antigen-presenting effects on BMDCs and heightened humoral and cellular immune responses compared with other formulations.

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Models to study basic and applied aspects of lysosomal storage disorders

Gaudioso

Silva

Ledesma

2022

Advanced Drug Delivery Reviews

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The enhanced immune response of PCV-2 vaccine using Rehmannia glutinosa polysaccharide liposome as an adjuvant

Cited by 24 publications

References 52 publications

<em>Rehmannia glutinosa</em> polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells

<em>Rehmannia glutinosa</em> polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells

Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres

Models to study basic and applied aspects of lysosomal storage disorders

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