The enhanced immunological activity of Paulownia tomentosa flower polysaccharide on Newcastle disease vaccine in chicken

Abstract

The extracts of Paulownia tomentosa (P. tomentosa) exhibit multiple pharmacological activities. In the present study, P. tomentosa flower polysaccharides (PTFP) were extracted by water decoction and ethanol precipitation, and the immunologic modulations of PTFP against Newcastle disease (ND) vaccine was investigated in chickens. The results showed that in a certain range of concentrations, PTFP treatment can dose-dependently enhance lymphocyte proliferation. Then, 280 14-days-old chickens were randomly divided into seven groups, and vaccinated with ND vaccine except blank control (BC) group. At the first vaccination, chickens were orally administrated with PTFP at concentration ranging from 0 to 50 mg/kg once a day for 3 successive days, and the BC group was treated with physiological saline. The lymphocyte proliferation rate, serum antibody titer, and levels of interferon-γ (IFN-γ) were respectively measured on 7, 14, 21, and 28 days after the first vaccination. The results showed that PTFP at the suitable doses could significantly promote lymphocyte proliferation, enhance serum antibody titer, and improve serum IFN-γ concentrations. Taken together, these data indicated that PTFP could improve the immune efficacy against ND vaccine in chickens, and could be as the candidate of a new-type immune adjuvant.

Paulownia tomentosa flower polysaccharide as an effective immunopotentiator to enhance immune responses for Newcastle disease vaccine in mice

“…Numerous studies have demonstrated that polysaccharides extracted from Chinese medicinal herbs could enhance immune responses and promote the efficiency of vaccines by oral administration ( Xie et al, 2012;Feng et al, 2013;Tang et al, 2019;Zhang et al, 2020). In our previous study, PTFP, extracted from P. tomentosa flower, had been proved to possess the immunomodulatory activity and enhance immune responses for orally administered ND-vaccine in chickens (Yang et al, 2019). Wang et al (2019) have reported that PTFP as a new immunopotentiator or adjuvant could enhance humoral and cellular responses in chickens by injection administration.…”

“…PTFP was prepared by water decoction and ethanol precipitation as previously described (Yang et al, 2019). In brief, dried cultured P. tomentosa flowers were extracted twice with boiling water for 2 hours and 1 hour, respectively.…”

“…. The in vivo experiment results demonstrated that PTFP could improve lymphocyte proliferation, increase antibody response, and enhance the secretion of interferon (IFN)-γ, indicating that PTFP has the potential to improve immune responses in ND-vaccinated chickens (Yang et al, 2019). However, the mechanism of PTFP enhancing immune responses for the ND vaccine is still unknown.…”

The Impact of Paulownia Leaves Extract on Performance, Blood Biochemical, Antioxidant, Immunological Indices, and Related Gene Expression of Broilers

“…The first treatment was fed the basal diet only without any PLE supplementation (served as a control), while the second, third, and fourth treatments were fed on a basal diet supplemented with 0.1, 0.3, and 0.5 mg g/kg diet of PLE, respectively. The concentrations examined were selected based on the results of Yang et al ( 17 ).…”

“…Furthermore, the high-flavonoid concentration of paulownia elongate leaf extracts, both dried and fresh, suggests that this plant has the potential for novel therapeutic applications against a broad range of oxidative disorders ( 12 ). Therefore, paulownia leaves, fruits, and flowers (a wood by-product) are the most important plant parts employed in the folk herbal medicine ( 16 , 17 ). According to a Chinese medical book edited by Li Shizhen in 1578, paulownia bark might be applied to treat hemorrhoids and worms infections, while the flowers could be used to reduce swelling and promote hair development ( 16 ).…”