The signed enhanced principal rank characteristic sequence (sepr-sequence) of an n × n Hermitian matrix is the sequence t 1 t 2 · · · t n , where t k is either A * , A + , A − , N, S * , S + , or S − , based on the following criteria: t k = A * if B has both a positive and a negative order-k principal minor, and each order-k principal minor is nonzero. t k = A + (respectively, t k = A − ) if each order-k principal minor is positive (respectively, negative). t k = N if each order-k principal minor is zero. t k = S * if B has each a positive, a negative, and a zero order-k principal minor. t k = S + (respectively, t k = S − ) if B has both a zero and a nonzero order-k principal minor, and each nonzero order-k principal minor is positive (respectively, negative). Such sequences provide more information than the (A, N, S) epr-sequence in the literature, where the kth term is either A, N, or S based on whether all, none, or some (but not all) of the order-k principal minors of the matrix are nonzero. Various sepr-sequences are shown to be unattainable by Hermitian matrices. In particular, by applying Muir's law of extensible minors, it is shown that subsequences such as A * N and NA * are prohibited in the sepr-sequence of a Hermitian matrix. For Hermitian matrices of orders n = 1, 2, 3, all attainable seprsequences are classified. For real symmetric matrices, a complete characterization of the attainable sepr-sequences whose underlying epr-sequence contains ANA as a nonterminal subsequence is established.