The Enhancement Effect of Calcium Ions on Ectomycorrhizal Fungi-Mediated Drought Resistance in Pinus sylvestris var. mongolica

Yin, Dafei; Wang, Helin; Jing, Qi

doi:10.1007/s00344-020-10197-y

Cited by 13 publications

(6 citation statements)

References 36 publications

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“…Those results suggest that S. placidus colonization appears to affect these functions of SOD and POD. A similar result was also reported in the studies of Chen et al [85] and Yin et al [86]. Though POD and SOD activities increased in response to water deficiency in NM seedlings, the increase was not able to detoxify drought-induced ROS, which can be demonstrated by higher MDA content under drought conditions [87].…”

Section: Pcassupporting

confidence: 87%

Role of Suillus placidus in Improving the Drought Tolerance of Masson Pine (Pinus massoniana Lamb.) Seedlings

Wang

Zhao

et al. 2021

Forests

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Masson pine is an important afforestation species in southern China, where seasonal drought is common. The present study focused on the effects of Suillus placidus, an ectomycorrhizal fungus, inoculation on the growth and physiological and biochemical performance of masson pine seedlings under four different watering treatments (well-watered, mild drought, moderate drought, and severe drought) to evaluate the symbiotic relationship between S. placidus and masson pine seedlings. Ectomycorrhizal-inoculated (ECM) and non-inoculated (NM) seedlings were grown in pots and maintained for 60 days using the weighing method. Results showed that seedlings' growth, dry weight, RWC, chlorophyll content, PSII efficiency, and photosynthesis decreased as drought stress intensified in both ECM and NM plants. This suggests that drought stress significantly limits the growth and photosynthetic performance of masson pine seedlings. Nevertheless, increased An/gs and proline contents in both NM and ECM prevented oxidative damage caused by drought stress. In addition, increased peroxidase (POD) activity is an essential defense mechanism of ECM seedling under drought stress. Compared with NM, ECM seedlings showed faster growth, higher RWC, and photosynthetic performance, and lower lipid peroxidation in cell membranes under drought stress, as indicated by higher POD activity and lower proline and malondialdehyde (MDA). Our experiment found that S. placidus inoculation can enhance the drought resistance of masson pine seedlings by increasing antioxidant enzyme activity, water use efficiency, and proline content, thereby enhancing growth under water-deficiency conditions. S. placidus can be used to cultivate high-quality seedlings and improve their survival in regions that experience seasonal droughts.

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Section: Pcassupporting

confidence: 87%

Role of Suillus placidus in Improving the Drought Tolerance of Masson Pine (Pinus massoniana Lamb.) Seedlings

Wang

Zhao

et al. 2021

Forests

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“…In addition, seeds were wrapped with sterile gauze for moisturizing and kept under 25 °C for germination, sprayed with sterile water every day until growth. Seeds were transferred to plastic pots filled with sterilized vermiculite: soil: sand mixture (1:2:1) and kept under the corresponding controlled greenhouse conditions ( Yin, Wang & Qi, 2021 ). After the seedlings were unearthed, fix the seedlings at 8 in each pot.…”

Section: Methodsmentioning

confidence: 99%

“…The root structure determines the efficiency of water absorption and transport of plants, which can help alleviate the damage caused by drought stress. Under drought stress, the root tip first perceives the signal and transmits it to the aboveground part ( Jia & Zhang, 2008 ; Yin, Wang & Qi, 2021 ). After drought, phenotypic characteristics such as total surface area, total volume, average diameter, total length, and biomass of plant roots change ( Zhang & Sun, 2016 ; Zhang et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

The effects of soil drought stress on growth characteristics, root system, and tissue anatomy of Pinus sylvestris var. mongolica

Meng¹,

Zhang²,

Yin³

2023

PeerJ

Self Cite

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The main purpose of this study was to study the changes in growth, root system, and tissue anatomical structure of Pinus sylvestris var. mongolica under soil drought conditions. In this study, the growth indexes and photosynthesis of P. sylvestris var. mongolica seedlings under soil drought stress were studied by pot cultivation. Continuous pot water control experiment of the indoor culture of P. sylvestris var. mongolica was carried out, ensuring that the soil water content of each treatment reached 80%, 40%, and 20% of the field moisture capacity as control, moderate drought and severe drought, respectively. The submicroscopic structures of the needles and roots were observed using a scanning electron microscope and a transmission electron microscope. The response of soil roots to drought stress was studied by root scanning. Moderate drought stress increased needle stomatal density, while under severe drought stress, stomatal density decreased. At the same time, the total number of root tips, total root length, root surface area, and root volume of seedlings decreased with the deepening of the drought. Furthermore, moderate drought and severe drought stress significantly reduced the chlorophyll a and chlorophyll b content in P. sylvestris var. mongolica seedlings compared to the control group. The needle cells were deformed and damaged, and chloroplasts and mitochondria were damaged, gradually disintegrated, and the number of osmiophiles increased. There was also an increase in nuclear vacuolation.

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“…This might be because after the 4 μm PS-MPs migrated to the surface of the mycelia, due to the large diameter of the particles, they could not penetrate into the fungal tissues and cells, aggregate with extracellular secretion of mycelia, adhere to the surface of mycelia, and disturb the normal absorption and operation of water and nutrients by ectomycorrhizal fungi. After symbiosis between mycorrhizal fungi and plant roots, the mycorrhizal fungi that form can promote the absorption of plant nutrients, including N, P, K and Mg (Yin et al, 2021;Gao et al, 2021;Khullar and Reddy, 2020). For example, in nutrient-poor soil, microplastics affect the absorption of K, P, Mg and sulfur (S) by plants.…”

Section: Effect Of Ps-mps On the Growth And N P K Contents Of Ld Myceliamentioning

confidence: 99%

Physiological response of ectomycorrhizal fungi (Lactarius delicious) to microplastics stress

2022

Global NEST: The International Journal

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<p>The pollution and toxicological effects of microplastics in soil have gradually attracted widespread attention, but studies on the ecological effects of microplastics on soil microorganisms and their mechanisms are still very scarce. To explore the physiological response of ectomycorrhizal fungi (Lactarius delicious, Ld) to microplastic stress, Ld was used as the test strain, and 80 nm and 4 μm monodisperse polystyrene microspheres (PS-MPs) were selected. The solid plate method and the liquid culture method were used to study the effect of PS-MPs with two particle sizes on the growth, nutrient content, reactive oxygen species (ROS) production, organic acid secretion and other indicators of the strain Ld. The results showed that with increasing PS-MPs concentration, the biomass of strain Ld decreased gradually. When the strain Ld was exposed to high concentrations of PS-MPs (200-300 mg L-1), PS-MPs with particle size of 4 μm showed stronger inhibition of biomass than PS-MPs with particle size of 80 nm. After exposure to the PS-MPs environment of two sizes, the concentration of PS-MPs increased (0-300 mg L-1), and the contents of phosphorus (P), potassium (K), H2O2 and superoxide anion radical (O2·-) in Ld mycelia increased significantly (p < 0.05). This showed that the antioxidant substances in Ld could not remove excess ROS, but could reduce the stress of microplastics by increasing the absorption of P and K from the environment, which was beneficial to its own metabolic activities. In addition, the strain Ld could secrete oxalic acid, acetic acid and succinic acid into the culture medium, which the amount of oxalic acid secreted was the highest. We speculated that strain Ld responded to the stress of microplastics by secreting a large amount of organic acids. Therefore, the mechanism of the influence of microplastics on ectomycorrhizal fungi may involve oxidative stress, and the mycelia responded to the toxicity of microplastics by secreting more organic acids and increasing the absorption of P and K. The results of this study will provide a scientific basis for the study of the acute toxicity of microplastics to ectomycorrhizal fungi.</p>

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The Enhancement Effect of Calcium Ions on Ectomycorrhizal Fungi-Mediated Drought Resistance in Pinus sylvestris var. mongolica

Cited by 13 publications

References 36 publications

Role of Suillus placidus in Improving the Drought Tolerance of Masson Pine (Pinus massoniana Lamb.) Seedlings

Role of Suillus placidus in Improving the Drought Tolerance of Masson Pine (Pinus massoniana Lamb.) Seedlings

The effects of soil drought stress on growth characteristics, root system, and tissue anatomy of Pinus sylvestris var. mongolica

Physiological response of ectomycorrhizal fungi (Lactarius delicious) to microplastics stress

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