The enhancement of the immune response against S. equi antigens through the intranasal administration of poly-ɛ-caprolactone-based nanoparticles

Genetic toxicology and preliminaryin vivostudies of nitric oxide donor tocopherol analogs as potential new class of antiatherogenic agents

“…For example, PCL nanospheres together with mucoadhesive polymers (alginate or chitosan) were used as a potential carrier for Streptococcus equi surface proteins. Serum IgG antibody levels of animals vaccinated with S. equi antigens encapsulated or adsorbed onto PCL particles were significantly higher than those caused by free antigens or even free antigens adjuvanted with cholera toxin B subunit [97]. Thus, although no examples of the use of PCL nanoparticles for allergen immunotherapy were found, the good results obtained by different authors by using these PCL-nanoparticles in the field of vaccination suggest these systems as potential adjuvants in allergen immunotherapy.…”

Comparison of Immunologic Responses Following Intranasal and Oral Administration of a USDA-Approved, Live-Attenuated Streptococcus equi Vaccine

“…The nanoparticles used were coupled with either mucoadhesive polymers (alginate or chitosan) or adsorption enhancers (spermine or oleic acid) to act as carriers for S. equi surface proteins. Mice vaccinated with PCL nanoparticles with especially mucoadhesive polymers had more robust systemic IgG and mucosal IgA than mice vaccinated with free antigen only (Florindo et al 2009). PCL nanoparticles are a potential delivery system for intranasal vaccinations.…”

“…A poly-ε-caprolactone (PCL)-based nanoparticle delivery system for intranasal administration was evaluated in mice (Florindo et al 2009). The PCL nanoparticles are biodegradable, increase the concentration of antigen to promote its interaction with antigen presenting cells, and protect antigens from proteolytic enzymes so they are useful as a vaccine delivery system especially to the MALT (Florindo et al 2009).…”

“…The PCL nanoparticles are biodegradable, increase the concentration of antigen to promote its interaction with antigen presenting cells, and protect antigens from proteolytic enzymes so they are useful as a vaccine delivery system especially to the MALT (Florindo et al 2009). The nanoparticles used were coupled with either mucoadhesive polymers (alginate or chitosan) or adsorption enhancers (spermine or oleic acid) to act as carriers for S. equi surface proteins.…”

The growing role of nanotechnology in combating infectious disease

“…65 Similar results were found when Streptococcus equi protein antigen and cholera toxin B immune adjuvant were encapsulated into PCL nanoparticles modified with chitosan, spermine or oleic acid adsorption enhancers, as chitosan-based particles induced a high serum IgG and secretory IgA response. 66 The high IgA response is thought to result from chitosan's strong interactions with sialic acid residues in mucin, which increases vaccine residence time and improves membrane transport by inducing the transient opening of mucosal tight junctions. 6,66 Chitosan formulations have also shown promise as immunostimulatory adjuvants in intradermal vaccinations.…”