The enzymatic basis for pesticide bioremediation

An enhanced understanding of the metal ion binding and active site structural features of phosphoesterases such as the glycerophosphodiesterase from Enterobacter aerogenes (GpdQ), and the organophosphate degrading agent from Agrobacterium radiobacter (OpdA) have important consequences for potential applications. Coupled with investigations of the metalloenzymes, programs of study to synthesise and characterise model complexes based on these metalloenzymes can add to our understanding of structure and function of the enzymes themselves. This review summarises some of our work and illustrates the significance and contributions of model studies to knowledge in the area.

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“…These enzymes have attracted attention as they are also able to hydrolyse highly toxic organophosphate nerve agents. [8][9][10][11] …”

Section: Phosphoesterases In Generalmentioning

confidence: 99%

“…58 The phosphate ester bond is prone to cleavage, mostly by hydrolysis, but photolytic degradation by sunlight is also possible; 59 however, biological pathways for decontamination have also attracted attention. 11,53,[60][61][62] …”

Section: Organophosphatesmentioning

confidence: 99%

Spectroscopic and mechanistic studies of dinuclear metallohydrolases and their biomimetic complexes

et al. 2014

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“…wide range of industries currently use enzymes in their processes, including food production, detergent manufacture, agriculture, chemical and pharmaceutical manufacture, textiles, leather, paper, diagnostics, medicine, and bioremediation (1,2). Although the demand for enzymes is increasing, the equipment and reagents required for their isolation and purification are expensive.…”

mentioning

confidence: 99%

In Vivo Self-Assembly of Stable Green Fluorescent Protein Fusion Particles and Their Uses in Enzyme Immobilization

Venning-Slater

Hooks

Rehm

2014

Appl Environ Microbiol

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Bacterial inclusion bodies are aggregations of mostly inactive and misfolded proteins. However, previously the in vivo self-assembly of green fluorescent protein (GFP) fusions into fluorescent particles which displayed specific binding sites suitable for applications in bioseparation and diagnostics was demonstrated. Here, the suitability of GFP particles for enzyme immobilization was assessed. The enzymes tested were a thermostable ␣-amylase from Bacillus licheniformis, N-acetyl-D-neuraminic acid aldolase (NanA) from Escherichia coli, and organophosphohydrolase (OpdA) from Agrobacterium radiobacter. Respective GFP particles were isolated and could be stably maintained outside the cell. These enzyme-bearing GFP particles exhibited considerable stability across a range of temperature, pH, and storage conditions and could be recycled. The ␣-amylase-bearing particles retained activity after treatments at 4 to 85°C and at pHs 4 to 10, were stable for 3 months at 4°C, and could be recycled up to three times. OpdA-bearing particles retained degradation activity after treatments at 4 to 45°C and at pHs 5 to 10 and were able to be recycled up to four times. In contrast, the performance of NanA-bearing particles rapidly declined (>50% loss) after each recycling step and 3 months storage at 4°C. However, they were still able to convert N-acetylmannosamine and pyruvate to Nacetylneuraminic acid after treatment at 4 to 85°C and at pHs 4 to 11. Fluorescent GFP fusion particles represent a novel method for the immobilization and display of enzymes. Potential applications include diagnostic assays, biomass conversion, pharmaceutical production, and bioremediation.

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“…Only preparations having the National Institute of Public Health -National Institute of Hygiene (NIPH -NIH) certificate can be used. Consequently, producing preparation is expensive and time consuming [41].…”

Section: Application Of Biopreparations and Enzymesmentioning

confidence: 99%

The ways to increase efficiency of soil bioremediation

Wołejko

Wydro

Loboda

2016

Ecological Chemistry and Engineering S

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Abstract:The aim of this paper was to present possibilities of using different substrates to assist the bioremediation of soils contaminated with heavy metals, pesticides and other substances. Today's bioengineering offers many solutions that enable the effective conduct of biological remediation, including both biostimulation and bioaugmentation. For this purpose, they are used to enrich various organic substances, sorbents, microbiological and enzymatic preparations, chemical substances of natural origin or nanoparticles. The use of genetic engineering as a tool to obtain microorganisms and plants capable of efficient degradation of pollutants may cause the risks that entails the introduction of transgenic plants and microorganisms into the environment. In order to determine the efficacy and possible effects of the various bioremediation techniques, it is required to conduct many studies and projects on a larger scale than only in the laboratory. Furthermore, it should be emphasized that bioremediation involves interdisciplinary issues and therefore, there is a need to combine knowledge from different disciplines, such as: microbiology, biochemistry, ecology, environmental engineering and process engineering.

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The enzymatic basis for pesticide bioremediation

Abstract: Enzymes are central to the biology of many pesticides, infl uencing their modes of action, environmental fates and mechanisms of target species resistance. Since

Cited by 153 publications

References 102 publications

Spectroscopic and mechanistic studies of dinuclear metallohydrolases and their biomimetic complexes

Spectroscopic and mechanistic studies of dinuclear metallohydrolases and their biomimetic complexes

In Vivo Self-Assembly of Stable Green Fluorescent Protein Fusion Particles and Their Uses in Enzyme Immobilization

The ways to increase efficiency of soil bioremediation

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