The epidemiology of Staphylococcus aureus infections from subclinical mastitis in dairy cows during a control programme

Sommerhäuser, Jürgen; Kloppert, B.; Wolter, W.; Zschöck, M.; Sobiraj, A.; Failing, Klaus

doi:10.1016/s0378-1135(03)00204-9

Cited by 88 publications

(87 citation statements)

References 31 publications

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“…Dessa maneira, a CCS é usada como critério de diagnóstico indireto de mastite subclínica 6 , sendo de extrema importância o seu controle e monitoramento, pois, além de fazer parte das exigências normativas de órgãos fiscalizadores do Brasil e de diversos outros países do mundo, este indicador está relacionado com a composição do leite, rendimento industrial e determinação da segurança alimentar do produto, enquanto que, para os produtores, é uma importante ferramenta no controle da saúde da glândula mamária, qualidade da matéria-prima e perda de produção 7,8 . Os principais mecanismos pelos quais ocorre modificação nas concentrações dos componentes do leite, com a elevação dos níveis de CCS, são as lesões às células do epitélio secretor, decorrente da mastite 9,10 , o que resulta em alteração da concentração de lactose, proteína e gordura [11][12][13] e em aumento da permeabilidade das células epiteliais, que determina a elevação da passagem de substâncias do sangue para o leite, tais como sódio, cloro, imunoglobulinas e outras proteínas séricas 9 . Evidentemente, as alterações qualitativas de produção irão depender da severidade da infecção e do estágio da doença; entretanto, há uma relação direta entre CCS e alterações na concentração dos constituintes do leite, justificando-se a realização de estudos que avaliem o impacto de diferentes níveis de CCS sobre indicadores da qualidade da matéria-prima.…”

Section: Introductionunclassified

Correlações entre contagem de células somáticas e parâmetros físico-químicos e microbiológicos de qualidade do leite

et al. 2014

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Objetivou-se avaliar as correlações entre contagem de células somáticas (CCS) e os constituintes do leite e verificar as associações das condições ambientais com a CCS. Os dados utilizados foram de 1.541 unidades produtoras de leite referentes a 15 municípios da bacia leiteira do Vale do Taquari, Rio Grande do Sul. Foram tabulados os dados de CCS, contagem bacteriana total (CBT) e composição centesimal do leite, referentes ao período de junho de 2008 a dezembro de 2011, totalizando 44.089 amostras analisadas. A temperatura ambiente apresentou correlação positiva e significativa com a CCS, enquanto a precipitação pluviométrica e a umidade relativa do ar, ausência de correlação. Os teores de gordura, proteína, minerais e sólidos totais foram diretamente correlacionados com a CCS, enquanto que os sólidos não gordurosos e a lactose apresentaram comportamento inverso. Através da análise de componentes principais (ACP), auxiliada pelo método hierárquico aglomerativo de agrupamento, os sete tratamentos presentes no estudo foram reduzidos a cinco grupos de acordo com a similaridade, permitindo constatar que amostras com CCS superiores a 400.000 até 750.000 céls mL-1, apresentam a mesma qualidade, não justificando a estratificação de intervalos nesta amplitude de variação.

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Section: Introductionunclassified

Correlações entre contagem de células somáticas e parâmetros físico-químicos e microbiológicos de qualidade do leite

et al. 2014

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“…Borges et al (2008) also found that 100% of the RRM samples from a dairy in Ceará (Brazil) were contaminated by CPS with values between 10 3 and 10 6 CFU/mL. According to Sommerhäuser et al (2003), the microbiological quality of milk is directly related to the hygiene of the milking process. The hygiene begins with the herd's health, since many illnesses of dairy cattle affect the original composition, flavor, smell, viscosity and microbiological quality of the milk.…”

Section: Discussionmentioning

confidence: 99%

Toxigenic Staphylococcus aureus in processing of coalho and mozzarella cheese

Pereira¹,

Machado²,

Lopes³

et al. 2017

Afr. J. Microbiol. Res.

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Staphylococcus aureus is commonly involved in food poisoning due to production of toxins responsible for causing animal and human diseases. In this study, 60 strains of presumptive S. aureus isolates from raw milk and cheese were biochemically identified in four dairies: 54 (90%) from refrigerated raw milk (RRM) with counts exceeding 10 6 CFU/mL, and six (10%) from cheese with similar concentrations of CFU/mL. Out of the 60 strains of presumptive S. aureus, 46 (76.7%) amplified the femA gene and then they were investigated regarding the presence of the Toxic Shock Syndrome Toxin-1 (TSST-1) gene and the classical enterotoxin genes (SEs) types A, B, C, D and E: 31 (67.4%) carried one or more encoding toxin genes, and 13 different genotypes were identified. Twenty-one strains (61.8%) carried one gene; three (8.8%), two genes; seven (20.6%), three genes; two (5.8%), four genes; and one (3%), five genes. The sec gene was the most frequent one, followed by seb and tst. The sed gene was expressed by 10 strains (29.4%), sea by five (14.7%) and see by three (8.8%). The S. aureus isolates showed genetic potential for producing toxins of importance for public health that presented a danger of food poisoning.

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“…Although some influence of the differences in the sampling methods and in the bacteriological analysis cannot be ruled out, this diversity and distribution of pulsotypes may indeed be explained by different management practices at the SEST. The absence of hand and udder washing and disinfection during the milking process certainly represents the most important problem, but other practices, such as the occasional exchange of the milkers between the herds, the mixing of all pregnant cows in the same maternity building and the introduction of recently purchased cows, also represent opportunities for new strains to be introduced and transferred between cows (Middleton et al 2002;Sommerhauser et al 2003;De Vliegher et al 2012). The majority of pulsotypes are identified during the four sampling events in 2010, but only A and B pulsotypes are present in the three herds from 2010 to 2012, the latter being isolated only once from the primiparous cows (Table 2).…”

Section: Discussionmentioning

confidence: 99%

A 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger

Issa

Duprez

Alambédji

et al. 2015

Trop Anim Health Prod

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Staphylococcus (S.) aureus is one of the most important pathogens causing bovine mastitis. The aim of the present work was to follow in three herds and during the 3 years the clonality of S. aureus isolated from California Mastitis Test (CMT)-positive cows at the experimental station of Toukounous (Niger) by (i) comparing their pulsed field gel electrophoresis (PFGE) fingerprints, (ii) identifying their virulotypes by PCR amplification and (iii) assessing the production of capsule and the formation of biofilm. The 88 S. aureus isolates belonged to 14 different pulsotypes, 3 of them being predominant: A (30 %), D (27 %), B (15 %). A and B pulsotypes had the highest profile similarity coefficient (94 %), while others had similarity coefficients under 60 %. Seventy-five S. aureus isolates were further studied for their virulotypes, capsular antigens and biofilm production. Most surface factor-, leukocidin-and haemolysin-, but not the enterotoxin-encoding genes were detected in the majority (>75 %) of the isolates and were evenly distributed between the A, B and D pulsotype isolates. The majority of the 72 S. aureus positive with the cap5H or cap8H PCR produced the CP5 (82 %) or the CP8 (88 %) capsular antigen, respectively. Biofilm production by the 57 icaA-positive isolates was strong for 8 isolates, moderate for 31 isolates but weak for 18 isolates, implying that the icaA gene may not be expressed in vitro by one third of the positive isolates. Similar to other studies, those results confirm that a restricted number of S. aureus clones circulate within the three herds at Toukounous and that their specific virulence-associated properties must still be further studied.

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The epidemiology of Staphylococcus aureus infections from subclinical mastitis in dairy cows during a control programme

Cited by 88 publications

References 31 publications

Correlações entre contagem de células somáticas e parâmetros físico-químicos e microbiológicos de qualidade do leite

Correlações entre contagem de células somáticas e parâmetros físico-químicos e microbiológicos de qualidade do leite

Toxigenic Staphylococcus aureus in processing of coalho and mozzarella cheese

A 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger

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