The Epidermal Barrier Structure and Function of Re-Harvested Skin from Non-Scalp Donor Sites

Zhang, Bo-Han; Luo, Peng; Sun, Jiachen; Li, Dawei; Liu, Zhaoxing; Liu, Xinzhu; Zhao, Hongqing; Li, Zhisheng; Xie, Xiaoye; Yang, Jianqiu; Shen, Chuanan

doi:10.1080/08941939.2022.2146318

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…The skin barrier is composed of tight junctions (TJ). TJs are connection structures between cells that exist in simple, multi-layered epithelia and endothelia and consist of transmembrane proteins (claudins, junctional adhesion molecule A (JAM-A), TJ-associated marvel proteins (TAMP) (occludin and tricellulin), and TJ plaque proteins (the zonula occludens proteins ZO-1 and ZO-2, MUPP-1, cingulin, and symplekin) [3]. It acts as a kind of barrier against the invasion of external allergens on the surface of the human body's skin.…”

Section: Introductionmentioning

confidence: 99%

Effects of Varying Ratios of Glycyrrhiza uralensis and Donkey Hide Gelatin Water Extracts on Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

Dong

Lee

et al. 2023

Nutrients

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Atopic dermatitis is a chronic skin disease that affects millions of people all over the world. The objective of this study was to evaluate the inhibitory effects of the roots of Glycyrrhiza uralensis (GU) and Donkey Hide Gelatin (DHG) water extracts on DNCB-induced NC/Nga mice and TNF-α/IFN-γ treated keratinocytes or LPS-stimulated macrophages. The combined treatment using the water extracts of GU and DHG improved the skin symptom evaluation score and skin histology, with increased expression of the skin barrier proteins Claudin 1 and Sirt 1 in lesion areas. The IFN-γ activity was promoted in PBMCs, ALN, and dorsal skin tissue, while the absolute cell number was reduced for T cells so that the production and expression of serum IgE and cytokines were suppressed. In TNF-α/IFN-γ induced HaCaT cells, IL-6, IL-8, MDC, and RANTES were all inhibited by GU and DHG water extracts, while ICAM-1 and COX-2 levels were similarly downregulated. In addition, GU and DHG water extracts decreased LPS-mediated nitric oxide, IL-6, TNF-α, and PGE2 in RAW 264.7 cells, and the expression of iNOS and COX-2 also decreased. Notably, the DHG:GU ratio of 4:1 was shown to have the best effects of all ratios. In conclusion, GU and DHG have anti-skin inflammatory potentials that can be used as alternative ingredients in the formula of functional foods for people with atopic dermatitis.

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Section: Introductionmentioning

confidence: 99%