The epidermal structure of Carassius gibelio: a link with ploidy status in spawning and postspawning periods

Halačka, Karel; Vetešník, Lukáš; Papoušek, Ivo; Mendel, Jan; Šimková, Andrea Vetešníková

doi:10.1111/j.1095-8649.2010.02833.x

Cited by 8 publications

(8 citation statements)

References 28 publications

(29 reference statements)

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“…Earlier works suggested that club cells are usually located in the middle of the epidermis (Iger and Abraham 1990), and that they do not communicate with the epidermal surface (Nakamura et al 2001, Halačka et al 2010 or communicate only when the epidermis has been ruptured by predation (Smith 1992). In this study, club cells on the surface of the epidermis released a viscous "secretion" in fishes belonging to groups 1 and 2 from, the fourth to the eleventh experimental days (Figs.…”

Section: Discussionmentioning

confidence: 89%

Alterations of Club Cell Activity in Epidermis of Common Carp, Cyprinus Carpio (Actinopterygii: Cypriniformes: Cyprinidae), due to Infection by Ichthyophthirius Multifiliis (Protista: Ciliophora)

Päkk

Hussar²,

Paaver³

2011

Acta Icth et Piscat

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Päkk P., Hussar P., Paaver T. 2011. Alterations of club cell activity in epidermis of common carp, Cyprinus carpio (Actinopterygii: Cypriniformes: Cyprinidae), due to infection by Ichthyophthirius multifiliis (Protista: Ciliophora). Acta Ichthyol. Piscat. 41 (3): 185-192.Background. The abundance of club cells in epidermal tissue of fishes in the superorder Ostariophysi is a poorly understood phenomenon. Previous results have suggested that epidermal club cells have a generic role in response to injury and that they display intense phagocytotic activity, having an anti-parasitic function in the host. Earlier works suggested that club cells are usually located in the middle of the epidermis and that they do not communicate with the epidermal surface or do it only when the epidermis has been ruptured by predation. The presently reported study focused on the alterations of club cell activity in carp epidermis induced by ectoparasite, Ichthyophthirius multifiliis. We hoped that our observations would help to understand the function(s) of these cells. Materials and methods. This study was based on 200 four-month old common carp, Cyprinus carpio L., with mean body weight of 65 ± 5 g. The fish were experimentally infected with theronts of Ichthyophthirius multifiliis. In sequential days post infection, samples of fins and body skin were collected for histological and histochemical examination. The correlation between club cell densities and mucous cell densities was analysed using Pearson correlation analyses. Results. A local reduction of mucous cells occurred after theront invasion-induced proliferation, and increased club cell density around the parasite during the growth of trophonts. After parasites left the skin due to salt-water treatment, a decrease in the number of club cells was detected. During reinvasion the decrease in parasite activity in areas of club cells proliferation was not noted. It was found that giant mature club cells were opened on the surface. Conclusion. Club cells have no anti-parasitic function against I. multifiliis and these mature cells released their viscous secretion into water. The high density of club cells in the epidermis compensates an overall low density or absence of mucous cells. As it can be hardly concluded that the function of club cells is phagocytic removal of cell debris, an integrated research on mucosal immune mechanisms, as well as studies on epidermal tissue responses on product(s) released by club cells ("alarm substance cells") should be carried out in the future.

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Section: Discussionmentioning

confidence: 89%

Alterations of Club Cell Activity in Epidermis of Common Carp, Cyprinus Carpio (Actinopterygii: Cypriniformes: Cyprinidae), due to Infection by Ichthyophthirius Multifiliis (Protista: Ciliophora)

Päkk

Hussar²,

Paaver³

2011

Acta Icth et Piscat

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“…Absolute epidermal thickness (A) and the relative proportion of secretory cells in a given volume of epidermis (B; calculated using a 10 μm morphometric web) were measured and used to compute the absolute values for secretory cells in the epidermis using the formula A × B/100 (Halačka et al , 2010. The relative thickness of the epidermis was calculated as the ratio of body size and absolute epidermal thickness (SL/A).…”

Section: Methodsmentioning

confidence: 99%

“…Low values have been found in grayling (Thymallus thymallus; 9% GC), rainbow trout (11% GC) and gibel carp (Carassius gibelio; 7% GC, 14% CC), while higher values have been observed in brown trout (29% GC) and pike (Esox lucius; 30% GC), and the highest values in European and Siberian bullhead (40-50% GC, 1-10% sacciform cells) and spined loach (Cobitis elongatoides; 22% GC, 44% CC) (Knoz and Halačka 1991;Halačka et al 2010). Our results of 28% GC for brook trout and 23% GC for the Arctic char hybrid, therefore, can be considered as relatively high.…”

Section: Goblet Cells [Gc] Club Cells [Cc] Sacciform Cells [Sc])mentioning

confidence: 99%

Epidermis structure in the brook trout (Salvelinus fontinalis) and its Arctic char (Salvelinus alpinus) hybrid

et al. 2015

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Brook trout (Salvelinus fontinalis) is a species of fish native to North-East America. Brook trout are also commercially raised in large numbers for food production. Skin infection and/or parasite outbreaks can have a serious economics effect on aquaculture businesses. For this reason, it has been hybridized with the more resistant Arctic char (Salvelinus alpinus). The aim of this study was an examination of the epidermal structure and dynamic in brook trout and its Arctic char hybrid which is less sensitive to skin infection. The samples of fish (72 brook trout, 72 brook trout × Arctic char hybrid) from fish farm in Pravíkov (49°19'10"N, 15°5'40"E) were collected five times during the year 2011. Absolute and relative epidermal thickness (in relation to body size) and relative proportion of secretory cells in a given volume of epidermis were measured. The epidermis structure of brook trout and brook trout × Arctic char hybrid both display similar seasonal dynamics, with a decrease in absolute and relative epidermal thickness and a reduction in the relative percentage of mucous secretory cells over the summer. On the other hand, the lower absolute (mean 103 µm (range 84-146 µm) in brook trout; 88 µm (range 68-115 µm) in hybrids) and relative epidermal thickness (mean 4.8 (range 3.6-6.8) in brook trout; 4.4 (range 2.9-6.4) in hybrids) and lower volume of secretory cells was observed to the hybrid (mean 28% (range 19-33%) in brook trout; 23% (range 10-30%) in hybrids). It can interrelate with their higher resistance to infection and/or parasite outbreaks. Fish, goblet cell, sacciform cell

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“…Mucous cells are commonly found in the epidermal, gill and intestinal epithelium [29,38], their frequency and secretory composition differing both between species and within species, or even within individuals, depending upon environment or individual body condition [38][39][40].…”

Section: Discussionmentioning

confidence: 99%

Differences in haematology and respiratory system morphology at three neogobiid species

Halačka

2015

Open Life Sciences

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Gill characteristics and the size and number of erythrocytes were observed for three species of goby: Neogobius melanostomus, N. fluviatilis and N. kessleri. While erythrocyte size was similar in all species, N. fluviatilis had a statistically higher erythrocyte count. Significant differences were found in gill apparatus structure, with N. melanostomus having 25% and 50% greater gill contact area than N. kessleri and N. fluviatilis, respectively. Secretory goblet cells, which produce a protective mucous, were also present in highest numbers in N. melanostomus gill tissues. These physiological and morphological differences may be one reason for differences observed in the invasiveness of these three species.

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The epidermal structure of Carassius gibelio: a link with ploidy status in spawning and postspawning periods

Cited by 8 publications

References 28 publications

Alterations of Club Cell Activity in Epidermis of Common Carp, <I>Cyprinus Carpio</I> (Actinopterygii: Cypriniformes: Cyprinidae), due to Infection by <I>Ichthyophthirius Multifiliis</I> (Protista: Ciliophora)

Alterations of Club Cell Activity in Epidermis of Common Carp, <I>Cyprinus Carpio</I> (Actinopterygii: Cypriniformes: Cyprinidae), due to Infection by <I>Ichthyophthirius Multifiliis</I> (Protista: Ciliophora)

Epidermis structure in the brook trout (Salvelinus fontinalis) and its Arctic char (Salvelinus alpinus) hybrid

Differences in haematology and respiratory system morphology at three neogobiid species

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