The epidermis‐specific cyclin CYCP3;1 is involved in the excess brassinosteroid signaling‐inhibited root meristem cell division

Chen, Yuxiao; Sun, Shi-Yong; Wang, Xuelu

doi:10.1111/jipb.12975

Cited by 15 publications

(7 citation statements)

References 37 publications

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“…The cell cycle is a regulatory network composed of a series of cell-cycle-related regulatory genes, in which cyclins are involved in the whole process, especially cell division. In this study, although all cyclins were found to have at least one cyclin box, only CYCU;1 had the CD20558 structural domain through CDD prediction (Table 1), and it contained Y(L/A)(E/A)RI(F/A)(R/K)(Y/F) and (N/S)VHRLL(V/I)T motifs [29,41]. In Arabidopsis, CYCPs may be involved in cell division, cell differentiation, and the nutritional status of the cell by interacting with CDKA1 [42].…”

Section: Cyclins Play a Role In Cell Divisionmentioning

confidence: 67%

Phenotypic Analysis and Molecular Characterization of Enlarged Cell Size Mutant in Nannochloropsis oceanica

Xu,

Lin,

Wang

et al. 2023

IJMS

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The cell cycle is the fundamental cellular process of eukaryotes. Although cell-cycle-related genes have been identified in microalgae, their cell cycle progression differs from species to species. Cell enlargement in microalgae is an essential biological trait. At the same time, there are various causes of cell enlargement, such as environmental factors, especially gene mutations. In this study, we first determined the phenotypic and biochemical characteristics of a previously obtained enlarged-cell-size mutant of Nannochloropsis oceanica, which was designated ECS. Whole-genome sequencing analysis of the insertion sites of ECS indicated that the insertion fragment is integrated inside the 5′-UTR of U/P-type cyclin CYCU;1 and significantly decreases the gene expression of this cyclin. In addition, the transcriptome showed that CYCU;1 is a highly expressed cyclin. Furthermore, cell cycle analysis and RT-qPCR of cell-cycle-related genes showed that ECS maintains a high proportion of 4C cells and a low proportion of 1C cells, and the expression level of CYCU;1 in wild-type (WT) cells is significantly increased at the end of the light phase and the beginning of the dark phase. This means that CYCU;1 is involved in cell division in the dark phase. Our results explain the reason for the larger ECS size. Mutation of CYCU;1 leads to the failure of ECS to fully complete cell division in the dark phase, resulting in an enlargement of the cell size and a decrease in cell density, which is helpful to understand the function of CYCU;1 in the Nannochloropsis cell cycle.

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Section: Cyclins Play a Role In Cell Divisionmentioning

confidence: 67%

Phenotypic Analysis and Molecular Characterization of Enlarged Cell Size Mutant in Nannochloropsis oceanica

Xu,

Lin,

Wang

et al. 2023

IJMS

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“…Model 1 includes genes involved in extracellular region, cell wall biosynthesis and lipid metabolism GO categories, implying the leaf base mainly involves growth related transcripts that accumulate at higher levels in leaf base than tip. For example, cycp3;1 , promotes cell division at the G2-M duration [ 24 ] and the expansin EXP16 is involved in cell wall expansion [ 25 ]. In contrast to this, a relatively high number of genes involved in photosynthesis related processes were highest expressed in the tip of the leaf (model 2), such as photosynthetic electron transport, chlorophyll binding, light signaling, with related genes, including OHP , OHP2 which are functionally lined to photosystem II biogenesis [ 26 ], and the early light-induced protein ELIP2 [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic analyses to summarize gene expression patterns that occur during leaf initiation of Chinese cabbage

Sun,

Liu,

Liu

et al. 2024

Horticulture Research

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In Chinese cabbage, rosette leaves expose their adaxial side to the light converting light energy into chemical energy, acting as a source for the growth of the leafy head. In the leafy head, the outer heading leaves expose their abaxial side to the light while the inner leaves are shielded from the light and have become a sink organ of the growing Chinese cabbage plant. Interestingly, variation in several ad/abaxial polarity genes is associated with the typical leafy head morphotype. The initiation of leaf primordia and the establishment of leaf ad/abaxial polarity are essential steps in the initiation of marginal meristem activity leading to leaf formation. Understanding the molecular genetic mechanisms of leaf primordia formation, polar differentiation, and leaf expansion is thus relevant to understand leafy head formation. As Brassica’s are mesa-hexaploids, many genes have multiple paralogues, complicating analysis of the genetic regulation of leaf development. In this study, we used laser dissection of Chinese cabbage leaf-primordia and the shoot apical meristem (SAM) to compare gene expression profiles between both adaxial and abaxial sides and the SAM aiming to capture transcriptome changes underlying leaf primordia development. We highlight genes with roles in hormone pathways and transcription factors. We also assessed gene expression gradients along expanded leaf blades from the same plants to analyze regulatory links between SAM, leaf primordia and the expanding rosette leaf. The catalogue of differentially expressed genes provides insights in gene expression patterns involved in leaf development and form a starting point to unravel leafy head formation.

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“…In this study, 20 genes were involved in cell growth and flower development. Examples for the cell growth: AC58 plays an important role in cell shape determination and cell division [57]; CYCP3-1 can regulate meristem cell division and lateral root development [58]; and MIZ1 participates in lateral root development [59]. For flower development, AP1 and AMP1 are involved in flower development [60,61]; TKPR2 is involved in pollen exine formation [62]; and HAT is essential for plant growth and development, especially in post-embryonic development [63].…”

Section: Discussionmentioning

confidence: 99%

Integrated Metabolites and Transcriptomics at Different Growth Stages Reveal Polysaccharide and Flavonoid Biosynthesis in Cynomorium songaricum

Wang

Wu³

et al. 2022

IJMS

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Cynomorium songaricum is a perennial parasitic herb, and its stem is widely used as a traditional Chinese medicine, which largely relies on bioactive compounds (e.g., polysaccharides, flavonoids, and triterpenes). To date, although the optimum harvest time of stems has been demonstrated at the unearthed stage (namely the early flowering stage, EFS), the accumulation mechanism of polysaccharides and flavonoids during growth stages is still limited. In this study, the physiological characteristics (stem fresh weight, contents of soluble sugar and flavonoids, and antioxidant capacity) at four different growth stages (germination stage (GS), vegetative growth stage (VGS), EFS, and flowering stage (FS)) were determined, transcriptomics were analyzed by illumina sequencing, and expression levels of key genes were validated by qRT-PCR at the GS, VGS, and EFS. The results show that the stem biomass, soluble sugar and total flavonoids contents, and antioxidant capacity peaked at EFS compared with GS, VGS, and FS. A total of 6098 and 13,023 differentially expressed genes (DEGs) were observed at VGS and EFS vs. GS, respectively, with 367 genes co-expressed. Based on their biological functions, 109 genes were directly involved in polysaccharide and flavonoid biosynthesis as well as growth and development. The expression levels of key genes involved in polysaccharides (e.g., GLCs, XTHs and PMEs), flavonoids (e.g., 4CLLs, CYPs and UGTs), growth and development (e.g., AC58, TCPs and AP1), hormones biosynthesis and signaling (e.g., YUC8, AIPT and ACO1), and transcription factors (e.g., MYBs, bHLHs and WRKYs) were in accordance with changes of physiological characteristics. The combinational analysis of metabolites with transcriptomics provides insight into the mechanism of polysaccharide and flavonoid biosynthesis in C. songaricum during growth stages.

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The epidermis‐specific cyclin CYCP3;1 is involved in the excess brassinosteroid signaling‐inhibited root meristem cell division

Cited by 15 publications

References 37 publications

Phenotypic Analysis and Molecular Characterization of Enlarged Cell Size Mutant in Nannochloropsis oceanica

Phenotypic Analysis and Molecular Characterization of Enlarged Cell Size Mutant in Nannochloropsis oceanica

Transcriptomic analyses to summarize gene expression patterns that occur during leaf initiation of Chinese cabbage

Integrated Metabolites and Transcriptomics at Different Growth Stages Reveal Polysaccharide and Flavonoid Biosynthesis in Cynomorium songaricum

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