The epithalamus of the developing and adult frog: calretinin expression and habenular asymmetry in Rana esculenta

Guglielmotti, Vittorio; Cristino, Luigia; Sada, E; Bentivoglio, Marina

doi:10.1016/j.brainres.2003.10.053

Cited by 15 publications

(10 citation statements)

References 50 publications

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“…In this regard, asymmetry in the bichir habenula is similar to that of CB-ir and CR-ir cells in the sturgeon habenula (Graña et al, 2012), and CR-ir cells in the mullet habenula (Díaz-Regueira and Anad on, 2000), although distribution of cells was different. Asymmetric distribution of CR immunoreactivity was also present in the frog habenula (Guglielmotti et al, 2004). As in the Siberian sturgeon (Graña et al, 2012), prominent CB immunoreactivity was observed in the periphery of the bichir right fasciculus retroflexus and dorsal neuropil of the interpeduncular nucleus, suggesting a conserved pattern.…”

Section: Cabps and The Organization Of The Bichir Habenulo-interpedunmentioning

confidence: 90%

Immunohistochemical distribution of calretinin and calbindin (D‐28k) in the brain of the cladistian Polypterus senegalus

Graña¹,

Folgueira²,

Huesa³

et al. 2013

J of Comparative Neurology

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Polypteriform fishes are believed to be basal to other living ray-finned bony fishes, and they may be useful for providing information of the neural organization that existed in the brain of the earliest ray-finned fishes. The calcium-binding proteins calretinin (CR) and calbindin-D28k (CB) have been widely used to characterize neuronal populations in vertebrate brains. Here, the distribution of the immunoreactivity against CR and CB was investigated in the olfactory organ and brain of Polypterus senegalus and compared to the distribution of these molecules in other ray-finned fishes. In general, CB-immunoreactive (ir) neurons were less abundant than CR-ir cells. CR immunohistochemistry revealed segregation of CR-ir olfactory receptor neurons in the olfactory mucosa and their bulbar projections. Our results confirmed important differences between pallial regions in terms of CR immunoreactivity of cell populations and afferent fibers. In the habenula, these calcium-binding proteins revealed right-left asymmetry of habenular subpopulations and segregation of their interpeduncular projections. CR immunohistochemistry distinguished among some thalamic, pretectal, and posterior tubercle-derived populations. Abundant CR-ir populations were observed in the midbrain, including the tectum. CR immunoreactivity was also useful for characterizing a putative secondary gustatory/visceral nucleus in the isthmus, and for distinguishing territories in the primary viscerosensory column and octavolateral region. Comparison of the data obtained within a segmental neuromeric context indicates that some CB-ir and CR-ir populations in polypteriform fishes are shared with other ray-finned fishes, but other positive structures appear to have evolved following the separation between polypterids and other ray-finned fishes.

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Section: Cabps and The Organization Of The Bichir Habenulo-interpedunmentioning

confidence: 90%

Immunohistochemical distribution of calretinin and calbindin (D‐28k) in the brain of the cladistian Polypterus senegalus

Graña¹,

Folgueira²,

Huesa³

et al. 2013

J of Comparative Neurology

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“…The medial habenular nuclei are subcompartmentalized, with particular subdomains possessing distinct cytoarchitectural and molecular properties (e.g., Villani et al, '94;Andres et al, '99;Tomizawa et al, 2001;Gamse et al, 2005). Although L-R differences in this organization have so far only been described for fish and frogs (Kemali and Guglielmotti, '77;Iwahori et al, '91a,b;Guglielmotti et al, 2004;Gamse et al, 2005;Aizawa et al, 2007), few molecular tools have been applied toward this problem in mammals. Asymmetry could also exist at the level of synaptic input onto individual IPN neurons.…”

Section: Dorsoventral Projection Pattern Of L-r Habenular Nuclei Is Nmentioning

confidence: 96%

Selective asymmetry in a conserved forebrain to midbrain projection

et al. 2007

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How the left and right sides of the brain acquire anatomical and functional specializations is not well understood. The zebrafish has proven to be a useful model to explore the genetic basis of neuroanatomical asymmetry in the developing forebrain. The dorsal diencephalon or epithalamus consists of the asymmetric pineal complex and adjacent paired nuclei, the left and right medial habenulae, which in zebrafish larvae, exhibit differences in their size, neuropil density and patterns of gene expression. In all vertebrates, axons from the medial habenular nuclei project within a prominent fiber bundle, the fasciculus retroflexus, to a shared midbrain target, the interpeduncular nucleus of the ventral tegmentum. However, in zebrafish, projections from the left habenula innervate the dorsal and ventral regions of the target nucleus, whereas right habenular efferents project only to the ventral region. A similar dorsoventral difference in habenular connectivity is found in another teleost species, the highly derived southern flounder, Paralichthys lethostima. In this flatfish, directional asymmetry of the habenular projection appears to be independent of the left-right morphology and orientation that an individual adopts post-metamorphosis. Comparative anterograde labeling of the brains of salamanders, frogs and mice reveals that axons emanating from the left and right medial habenulae do not project to different domains, but rather, they traverse the target nucleus in a complementary mirror image pattern. Thus, although the habenulo-interpeduncular conduction system is highly conserved in the vertebrate brain, the stereotypic dorsoventral topography of left-right connections appears to be a feature that is specific to teleosts.

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“…A lateral subnucleus occupies most of the dorsal left habenula in the stingray brain, but is restricted to a smaller medial posterior region on the right (Iwahori et al, 1991a;Iwahori et al, 1991b). There is also a dorsoventral difference in the distribution of the calcium-binding protein calretinin A in the left habenula of Rana esculenta (Guglielmotti et al, 2004). The significance of asymmetry and the relationship of gene expression subdomains with afferent input, habenular ultrastructure, and connectivity are important issues to pursue.…”

Section: Leftover-related Gene Expression Defines Habenular Subdomainsmentioning

confidence: 99%

Directional asymmetry of the zebrafish epithalamus guides dorsoventral innervation of the midbrain target

et al. 2005

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The zebrafish epithalamus, consisting of the pineal complex and flanking dorsal habenular nuclei, provides a valuable model for exploring how left-right differences could arise in the vertebrate brain. The parapineal lies to the left of the pineal and the left habenula is larger, has expanded dense neuropil, and distinct patterns of gene expression from the right habenula. Under the influence of Nodal signaling, positioning of the parapineal sets the direction of habenular asymmetry and thereby determines the left-right origin of habenular projections onto the midbrain target, the interpeduncular nucleus (IPN). In zebrafish with parapineal reversal, neurons from the left habenula project to a more limited ventral IPN region where right habenular axons would normally project. Conversely, efferents from the right habenula adopt a more extensive dorsoventral IPN projection pattern typical of left habenular neurons. Three members of the leftover-related KCTD (potassium channel tetramerization domain containing) gene family are expressed differently by the left and right habenula, in patterns that define asymmetric subnuclei. Molecular asymmetry extends to protein levels in habenular efferents, providing additional evidence that left and right axons terminate within different dorsoventral regions of the midbrain target. Laser-mediated ablation of the parapineal disrupts habenular asymmetry and consequently alters the dorsoventral distribution of innervating axons. The results demonstrate that laterality of the dorsal forebrain influences the formation of midbrain connections and their molecular properties.

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The epithalamus of the developing and adult frog: calretinin expression and habenular asymmetry in Rana esculenta

Cited by 15 publications

References 50 publications

Immunohistochemical distribution of calretinin and calbindin (D‐28k) in the brain of the cladistian Polypterus senegalus

Immunohistochemical distribution of calretinin and calbindin (D‐28k) in the brain of the cladistian Polypterus senegalus

Selective asymmetry in a conserved forebrain to midbrain projection

Directional asymmetry of the zebrafish epithalamus guides dorsoventral innervation of the midbrain target

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