The Equilibria Between Monovalent Ions and Phosphatidylcholine Monolayer at the Air/Water Interface

Petelska, Aneta D.; Figaszewski, Zbigniew A.

doi:10.1007/s00232-013-9558-3

Cited by 11 publications

(10 citation statements)

References 22 publications

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“…This work continues the systematic study of electrical properties of human erythrocytes and thrombocytes membranes realized by Figaszewski and co-workers (Petelska et al 2012 ; Kotyńska et al 2012 ; Szeremeta et al 2013 ). In our previous paper (Szeremeta et al 2013 ), we presented changes of the surface charge of blood cells after fatal carbon monoxide poisoning obtained only on the experimental way. The experiment was performed using microelectrophoresis method, which is one of the basic analytical tools for biological studies.…”

Section: Introductionsupporting

confidence: 79%

“…However, the processes change significantly under the influence of various toxic factors as well as physiological and non-physiological conditions of the body. Many reports in the literature have shown changes in the structure and physicochemical properties of biological membranes, which are observed in physiological processes such as aging and leads to death as a consequence (Kala and Chudzikiewicz 2003 ) and in pathological, such as cardiovascular disease (Langlois 2009 ), tumors (Madej 1999 ; Szachowicz-Petelska et al 2010 ), fatal carbon monoxide poisoning (Weaver 2009 ; Szeremeta et al 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Fatal Carbon Monoxide Poisoning on the Equilibria Between Cell Membranes and the Electrolyte Solution

2014

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The effect of fatal carbon monoxide poisoning on equilibria between cell membranes and surrounding ions was described using a theoretical four-equilibria model. The model was developed to obtain parameters characterizing the interactions between solution ions and erythrocyte or thrombocyte membrane surface. The parameters are the total surface concentrations of both acidic and basic groups CA, CB and their association constants with solution ions KAH, KBOH. These parameters were used to calculate the theoretical values of surface charge density. The model was validated by comparison of these values to experimental data, which were determined from the electrophoretic mobility measurements of the blood cells. The experimental and theoretical surface charge density values agree at pH 2–8, and at higher pH, the deviation was observed.

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Section: Introductionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

The Effect of Fatal Carbon Monoxide Poisoning on the Equilibria Between Cell Membranes and the Electrolyte Solution

2014

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“…MD simulations support this, with many suggesting that at low concentrations ($0.2 M) K + only weakly interacts, if at all, with PC headgroups, while Na + strongly interacts [46,47]. Equilibria calculations, however, find that PC-K + complexes are more stable than PC-Na + complexes [48]. No definitive conclusion on relative binding affinity of Na + vs. K + to DPPC can be made from P-Area isotherms presented here as contrary DA values are observed between 0.6 and 2.0 M solutions.…”

Section: Resultsmentioning

confidence: 53%

Effect of cation enrichment on dipalmitoylphosphatidylcholine (DPPC) monolayers at the air-water interface

Adams

Casper

Allen

2016

Journal of Colloid and Interface Science

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The effect of highly concentrated salt solutions of marine-relevant cations (Na(+), K(+), Mg(2+), and Ca(2+)) on Langmuir monolayers of dipalmitoylphosphatidylcholine (DPPC) was investigated by means of surface pressure-area isotherms, Brewster angle microscopy (BAM), and infrared reflection-absorption spectroscopy (IRRAS). It was found that monovalent cations and Mg(2+) have similar phase behavior, causing DPPC monolayers to expand, while Ca(2+) induces condensation. All cations disrupted the surface morphology at high cation concentration, resulting in decreased reflectivity from the monolayer. Monolayer refractive index was calculated from BAM image intensity in the liquid condensed phase and decreased with increasing cation concentration, which suggests that orientation of the alkyl chains change. Monovalent ions increase ordering of the alkyl chains, more than divalents, yet have little interaction with the DPPC headgroup. Mg(2+) induces gauche defects in the alkyl chain and increases headgroup hydration at low lipid coverage but increases chain ordering and dehydrates the headgroup at high lipid coverage. Ca(2+) orders alkyl chains and dehydrates the phosphate moiety, independent of lipid phase. At the highest salt concentration investigated, significant narrowing of the asymmetric PO2(-) vibrational mode occurs and is attributed to considerable dehydration of the DPPC headgroup.

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“…Ion adsorption on the lipid membrane surface has been explored through various techniques, including nuclear magnetic resonance [8,9], Langmuir method [10][11][12] microelectrophoresis [13][14][15] and computational methods, such as molecular dynamics simulations [16,17]. In particular, microelectrophoresis can be used to determine membranes surface charge density values.…”

Section: Introductionmentioning

confidence: 99%

Microelectrophoretic investigation of the interactions between liposomal membranes formed from a phosphatidylcholine-phosphatidylglycerol mixture and monovalent ions

Kotyńska

Figaszewski

2014

Eur. Phys. J. E

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Abstract. In this paper, we characterized the interactions between two-component liposomal membranes and monovalent electrolyte ions. Liposomes were formed from neutral (phosphatidylcholine) and anionic (phosphatidylglycerol) lipids mixed in various ratios. Microelectrophoresis was used to determine the dependence of the membrane surface charge density on the pH of the electrolyte solution. Changes in the membrane electric charge caused by the adsorption of Na + , Cl − , H + , and OH − ions were observed, and the equilibria among these ions and the phosphatidylcholine-phosphatidylglycerol membrane surface were quantified. We proposed a mathematical model for characterizing these equilibria. Using this model, together with experimental data of the membrane surface charge density, we determined association constants characterizing the equilibria. Knowledge of these parameters was necessary to calculate the theoretical curves of the model. We validated the model by curve-fitting the experimental data points to simulated data generated by the model.

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The Equilibria Between Monovalent Ions and Phosphatidylcholine Monolayer at the Air/Water Interface

Cited by 11 publications

References 22 publications

The Effect of Fatal Carbon Monoxide Poisoning on the Equilibria Between Cell Membranes and the Electrolyte Solution

The Effect of Fatal Carbon Monoxide Poisoning on the Equilibria Between Cell Membranes and the Electrolyte Solution

Effect of cation enrichment on dipalmitoylphosphatidylcholine (DPPC) monolayers at the air-water interface

Microelectrophoretic investigation of the interactions between liposomal membranes formed from a phosphatidylcholine-phosphatidylglycerol mixture and monovalent ions

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