The ER Lumenal Hsp70 Protein FpLhs1 Is Important for Conidiation and Plant Infection in Fusarium pseudograminearum

Abstract

Heat shock protein 70s (Hsp70s) are a class of molecular chaperones that are highly conserved and ubiquitous in organisms ranging from microorganisms to plants and humans. Hsp70s play key roles in cellular development and protecting living organisms from environmental stresses such as heat, drought, salinity, acidity, and cold. However, their functions in pathogenic fungi are largely unknown. Here, a total of 14 FpHsp70 genes were identified in Fusarium pseudograminearum , including 3 in the mitochondria, 7 in the cytoplasm, 2 in the endoplasmic reticulum (ER), 1 in the nucleus, and 1 in the plastid. However, the exon–intron boundaries and protein motifs of the FpHsp70 have no consistency in the same subfamily. Expression analysis revealed that most FpHsp70 genes were up-regulated during infection, implying that FpHsp70 genes may play important roles in F . pseudograminearum pathogenicity. Furthermore, knockout of an ER lumenal Hsp70 homolog FpLhs1 gene reduced growth, conidiation, and pathogenicity in F . pseudograminearum . These mutants also showed a defect in secretion of some proteins. Together, FpHsp70s might play essential roles in F . pseudograminearum and FpLhs1 is likely to act on the development and virulence by regulating protein secretion.

The bZIP transcription factor FpAda1 is essential for fungal growth and conidiation in Fusarium pseudograminearum

“…The split-marker approach was used to generate genereplacement constructs for the FpAda1 gene as described in our previous study (Chen et al 2019a). Primers are listed in Supplementary Table S1 and a schematic diagram of primers located for gene replacement with split-marker strategy and screening of mutant is shown in Fig.…”

Comparative Genomic Analysis of Capsule-Producing Black Yeasts Exophiala dermatitidis and Exophiala spinifera, Potential Agents of Disseminated Mycoses

“…Studies in Fusarium showed that knockout of an ER lumenal Hsp70 homolog FpLhs1 gene reduced growth, conidiation, and pathogenicity. FpLhs1 is likely to act on the development and virulence by regulating protein secretion (Chen et al, 2019). The ubiquitin family is also essential in both species.…”

“…Gene families PFam and IPR in the literature have been associated with virulence; strains were found to differ rather considerably in the presence of these genes (Table 4). CAP Gly domain, cysteine-rich secretory protein family, Hsp70 protein, mRNA capping enzyme and ubiquitin family were consistently present with higher copy numbers (Choudhary and Schneiter, 2012;Chen et al, 2019). From the virulence gene prediction, the adenylate cyclase associated (CAP) Gly-domain was present in both species, while capsular polysaccharide synthesis protein and CAP N-terminal were present only in some of the E. dermatitidis strains.…”

TMT-based Quantitative Proteomic Analysis Reveals Defense Mechanism of Wheat Against the Crown Rot Pathogen Fusarium Pseudograminearum

“…Zhou et al investigated the distribution and diversity of the pathogens associated with Fusarium crown rot in the Huanghuai wheat-growing region of China and found that F. pseudograminearum was the dominant species [11]. Several studies have con rmed that ER Lumenal Hsp70 protein FpLhs1, transcription factor FpAda1, and FpNPS9 are important for F. pseudograminearum infection [12][13][14]. FCR resistance responses in wheat are complex and controlled by multiple quantitative trait loci (QTLs) [15].…”