The erythrocyte sedimentation curve: A semi-empirical approach1

Puccini, C; Stasiw, D.M.; Cerny, L.C.

doi:10.3233/bir-1977-14105

Cited by 25 publications

(7 citation statements)

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“…Also shown in Fig 7A is a fit of the semi-empirical model proposed by Puccini et al . [ 6 ] to the data: where h p is the height of the plasma zone, h ∞ is the limit to the growth of the plasma zone, t 50 is the half-time of sedimentation, β is the exponent, and t is time of sedimentation. The obtained fitting parameters were h ∞ = 3.252 mm, t 50 = 5084 s, and β = 1.344.…”

Section: Resultsmentioning

confidence: 99%

Effects of Aggregation on Blood Sedimentation and Conductivity

Zhbanov

Yang

2015

PLoS ONE

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The erythrocyte sedimentation rate (ESR) test has been used for over a century. The Westergren method is routinely used in a variety of clinics. However, the mechanism of erythrocyte sedimentation remains unclear, and the 60 min required for the test seems excessive. We investigated the effects of cell aggregation during blood sedimentation and electrical conductivity at different hematocrits. A sample of blood was drop cast into a small chamber with two planar electrodes placed on the bottom. The measured blood conductivity increased slightly during the first minute and decreased thereafter. We explored various methods of enhancing or retarding the erythrocyte aggregation. Using experimental measurements and theoretical calculations, we show that the initial increase in blood conductivity was indeed caused by aggregation, while the subsequent decrease in conductivity resulted from the deposition of erythrocytes. We present a method for calculating blood conductivity based on effective medium theory. Erythrocytes are modeled as conducting spheroids surrounded by a thin insulating membrane. A digital camera was used to investigate the erythrocyte sedimentation behavior and the distribution of the cell volume fraction in a capillary tube. Experimental observations and theoretical estimations of the settling velocity are provided. We experimentally demonstrate that the disaggregated cells settle much slower than the aggregated cells. We show that our method of measuring the electrical conductivity credibly reflected the ESR. The method was very sensitive to the initial stage of aggregation and sedimentation, while the sedimentation curve for the Westergren ESR test has a very mild slope in the initial time. We tested our method for rapid estimation of the Westergren ESR. We show a correlation between our method of measuring changes in blood conductivity and standard Westergren ESR method. In the future, our method could be examined as a potential means of accelerating ESR tests in clinical practice.

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Section: Resultsmentioning

confidence: 99%

Effects of Aggregation on Blood Sedimentation and Conductivity

Zhbanov

Yang

2015

PLoS ONE

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“…Several authors made efforts to model the settling of suspensions by extending Stokes' equation or postulated empirical equations (Adamson andGlasson 1925, Robinson 1926, and McCabe 1937, Ward and Kammermeyer 1940, Work and Kohler 1940, but no further important contributions were made until the forties. It is interesting to note that Robinson's paper was of minor inf luence for the development of sedimentation theory in the context of mineral processing, but that its approach seems to have been of great interest to investigators in the area of blood sedimentation half a century later (Puccini et al 1977). Stewart and Roberts (1933) give, in a review paper, a good idea of the state of the art of thickening in the twenties.…”

Section: The Invention Of the Dorr Thickener Andmentioning

confidence: 99%

A Century of Research in Sedimentation and Thickening

Concha

Bürger

2002

KONA

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“…tation(0 T), P`0X005). The shape of the curves was sigmoidal [Puccini et al, 1977] even for a large value of p iso /p. According to the previously described procedure [Long et al, 1990;Iino, 1997], the value of the maximum slope of the sedimentation curve, V max , was calculated and shown in Figure 5.…”

Section: Esr In Plasma Solutionmentioning

confidence: 92%

Osmolality dependence of erythrocyte sedimentation and aggregation in a strong magnetic field

Iino

Okuda

2000

Bioelectromagnetics

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In order to specify the major determinant of the magnetic enhancement of erythrocyte sedimentation observed previously, the dependence of erythrocyte sedimentation rate (ESR) on osmolality was measured under a strong magnetic field. Even at hypotonic osmolality, an increase in ESR due to aggregation was observed in plasma solution as compared with that without aggregation in saline solution. However, the magnetic field did not enhance ESR at hypotonic osmolality, when the cell shape was an isotropic sphere (spherocyte). Thus, we narrowed our search to a mechanism that would explain the enhanced ESR found specifically in anisotropic erythrocytes. It was concluded that the major determinant can only work for anisotropic erythrocytes and is a magnetic field-induced increase in an intermembrane adhesive area due to magnetic orientation of anisotropic erythrocytes.

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The erythrocyte sedimentation curve: A semi-empirical approach1

Cited by 25 publications

References 0 publications

Effects of Aggregation on Blood Sedimentation and Conductivity

Effects of Aggregation on Blood Sedimentation and Conductivity

A Century of Research in Sedimentation and Thickening

Osmolality dependence of erythrocyte sedimentation and aggregation in a strong magnetic field

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