The essential genome of Ralstonia solanacearum

Su, Yaxing; Xu, Yanan; Li, Qiqin; Yuan, Gonglin; Zheng, Danxing

doi:10.1016/j.micres.2020.126500

Cited by 15 publications

(12 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We previously constructed a near-saturated transposon insertion library of R. solanacearum GMI1000 with approximately 240,000 individual insertion mutants, covering 70.44% to 80.96% of all potential insertion sites ( 20 ). An in planta Tn-seq analysis was conducted using this near-saturated transposon insertion library to identify R. solanacearum genes essential for in planta survival.…”

Section: Resultsmentioning

confidence: 99%

“…The recovered transposon insertion library was referred to as “ in vivo .” Total DNAs of the transposon insertion libraries before and after infection were extracted and divided into two groups for technical replicates. The total DNA samples were subjected to MmeI digestion, adapter ligation, and PCR amplification to construct Illumina sequencing libraries and subjected to sequencing and raw data preprocessing on the Galaxy web platform as previously described ( 20 , 43 ). The index sequences of the sequencing manufacturer and transposon sequence in raw reads were trimmed.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Genome-Wide Identification of Ralstonia solanacearum Genes Required for Survival in Tomato Plants

Liang

et al. 2021

mSystems

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification of Ralstonia solanacearum Genes Required for Survival in Tomato Plants

Liang

et al. 2021

mSystems

Self Cite

View full text Add to dashboard Cite

show abstract

“…Further, the interaction of a network of chemicals-proteins was analyzed using the STITCH tool to predict the target proteins of R. solanacerum for the antibacterial compounds ( Kuhn et al, 2008 ). Finally, the target proteins were blasted against the essential genes of the bacterium to identify the essential genes using the BlastP program of the DEG database ( Luo et al, 2014 , Su et al, 2020 ). The proteins encoded by these essential genes were selected for further analysis.…”

Section: Methodsmentioning

confidence: 99%

Molecular identification and biological control of Ralstonia solanacearum from wilt of papaya by natural compounds and Bacillus subtilis: An integrated experimental and computational study

Hossain

Billah

Ali

et al. 2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Ralstonia solanacearum is a harmful pathogen that causes severe wilt disease in several vegetables. In the present study, we identified R. solanacearum from wilt of papaya by 16S rRNA PCR amplification. Virulence ability of R. solanacearum was determined by amplification of approximately 1500 bp clear band of hrpB gene. Further, in-vitro seed germination assay showed that R. solanacearum reduced the germination rate up to 26.21%, 34% and 33.63% of cucumber, bottle guard and pumpkin seeds, respectively whereas shoot and root growth were also significantly decreased. Moreover, growth inhibition of R. solanacearum was recorded using antibacterial compound from medicinal plant and antagonistic B. subtilis. Petroleum ether root extract of Rauvolfia serpentina showed highest 22 ± 0.04 mm diameter of zone of inhibition where methanolic extract of Cymbopogon citratus and ethanolic extract of Lantana camara exhibited 20 ± 0.06 mm and 20 ± 0.01 mm zone of inhibition against R. solanacearum , respectively . In addition, bioactive compounds of B. subtilis inhibited R. solanacearum growth by generating 17 ± 0.09 mm zone of inhibition. To unveil the inhibition mechanism, we adopted chemical-protein interaction network and molecular docking approaches where we found that, rutin from C. citratus interacts with citrate (Si)-synthase and dihydrolipoyl dehydrogenase of R. solanacearum with binding affinity of −9.7 kcal/mol and −9.5 kcal/mol while quercetin from B. subtillis interacts with the essential protein F0F1 ATP synthase subunit alpha of the R. solancearum with binding affinity of −6.9 kcal/mol and inhibit the growth of R. solanacearum . Our study will give shed light on the development of eco-friendly biological control of wilt disease of papaya.

show abstract

“…TALEs (transcription activation-like effectors) in pathogens are the natural triggers for E-gene-mediated disease resistance. They exist only in plant phytopathogenic Xanthomonas and Ralstonia, which cause diseases in many plant species, including important crops [15,16]. TALEs belong to a family of structurally conserved type III effectors and function as eukaryotic transcription factors in inducing gene expression.…”

Section: Introductionmentioning

confidence: 99%

Plant Executor Genes

Guo

Chen

et al. 2022

IJMS

View full text Add to dashboard Cite

Executor (E) genes comprise a new type of plant resistance (R) genes, identified from host–Xanthomonas interactions. The Xanthomonas-secreted transcription activation-like effectors (TALEs) usually function as major virulence factors, which activate the expression of the so-called “susceptibility” (S) genes for disease development. This activation is achieved via the binding of the TALEs to the effector-binding element (EBE) in the S gene promoter. However, host plants have evolved EBEs in the promoters of some otherwise silent R genes, whose expression directly causes a host cell death that is characterized by a hypersensitive response (HR). Such R genes are called E genes because they trap the pathogen TALEs in order to activate expression, and the resulting HR prevents pathogen growth and disease development. Currently, deploying E gene resistance is becoming a major component in disease resistance breeding, especially for rice bacterial blight resistance. Currently, the biochemical mechanisms, or the working pathways of the E proteins, are still fuzzy. There is no significant nucleotide sequence homology among E genes, although E proteins share some structural motifs that are probably associated with the signal transduction in the effector-triggered immunity. Here, we summarize the current knowledge regarding TALE-type avirulence proteins, E gene activation, the E protein structural traits, and the classification of E genes, in order to sharpen our understanding of the plant E genes.

show abstract

The essential genome of Ralstonia solanacearum

Cited by 15 publications

References 30 publications

Genome-Wide Identification of Ralstonia solanacearum Genes Required for Survival in Tomato Plants

Genome-Wide Identification of Ralstonia solanacearum Genes Required for Survival in Tomato Plants

Molecular identification and biological control of Ralstonia solanacearum from wilt of papaya by natural compounds and Bacillus subtilis: An integrated experimental and computational study

Plant Executor Genes

Contact Info

Product

Resources

About