The Essential Oil of &amp;lt;i&amp;gt;Eucalyptus grandis&amp;lt;/i&amp;gt; W. Hill ex Maiden Inhibits Microbial Growth by Inducing Membrane Damage

Soyingbe, Oluwagbemiga Sewanu; Oyedeji, Adebola O.; Basson, A. K.; Opoku, Andy R.

doi:10.4236/cm.2013.41002

Cited by 18 publications

(10 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cytosolic LDH release assay was done using microplate technique previously described [ 22 – 25 ]. The LDH release assay was calculated with the formula: (E-C)/(T-C) × 100, where E is the absorbance of the experimental cell cultures, C is the absorbance of the cell medium used as the control, and T the absorbance of the positive control which is the LDH release of Triton X-100 lysed cells.…”

Section: Methodsmentioning

confidence: 99%

In vitro antibacterial and cytotoxic activity of leaf extracts of Centella asiatica (L.) Urb, Warburgia salutaris (Bertol. F.) Chiov and Curtisia dentata (Burm. F.) C.A.Sm - medicinal plants used in South Africa

Soyingbe¹,

Mongalo²,

Makhafola³

2018

BMC Complement Altern Med

Self Cite

View full text Add to dashboard Cite

BackgroundCompounds having both anticancer and antimicrobial activity have promising therapeutic potential due to their selective cytotoxicity and their potential to reduce the occurrence of bacterial and fungal infections in immune-compromised cancer patients. In our quest to find new antimicrobial agents with potent anticancer activity, the biological potential of leaves from the three medicinal plants Centella asiatica, Warburgia salutaris and Curtisia dentata as used by Zulu traditional healers for the treatment of cancer is investigated.MethodsExtracts were assayed for antibacterial activity using the agar well diffusion and micro plate dilution assay. In addition, minimum bactericidal concentrations (MBC), lactate dehydrogenase (LDH) release assay and rhodamine 6G intake assay were used to ascertain the antibacterial activity. The cytotoxic effects of the plant extracts were determined using tetrazolium-based colorimetric (MTT) cell proliferation assay against MCF-7, human colorectal carcinoma cells (Caco-2), A549 and HeLa cancerous cell lines.ResultsThe acetone extracts from Waburgia salutaris revealed noteworthy anti-proliferative effect yielding IC50 value of 34.15 μg/ml against MCF-7 cell line, while acetone extract from Curtisia dentata significantly (P ≤ 0.05) revealed promising IC50 values of 41.55, 45.13, 57.35 and 43.24 μg/ml against A549, HeLa, CaCo-2 and MCF-7 cell lines. The extracts further revealed a broad-spectrum antibacterial activity against bacterial strains used in the study. An acetone extract from W. salutaris revealed the highest zone of inhibition and the lowest minimum inhibitory concentration (MIC) of 21.0 mm and 0.16 mg/ml respectively against Staphylococcus aureus. Methanol extract from W. salutaris and ethyl acetate extract from C. dentata revealed 53% inhibition of R6G inside the cell against Staphylococcus aureus and Escherichia coli respectively in a cytosolic lactate dehydrogenase assay, suggesting that the mode of action of such extracts may be through efflux pump.ConclusionsOverall, the extracts had good antibacterial activity and anti-proliferative effects against selected cancerous cell lines. Given the good antibacterial activity of the extracts the plants may act as an immune booster and prevent infection in immunosuppressed cancer patients. This is further supported by the plants’ anti-proliferative potential, bacteriostatic, bactericidal properties and also their ability to block bacterial efflux pump systems.

show abstract

Section: Methodsmentioning

confidence: 99%

In vitro antibacterial and cytotoxic activity of leaf extracts of Centella asiatica (L.) Urb, Warburgia salutaris (Bertol. F.) Chiov and Curtisia dentata (Burm. F.) C.A.Sm - medicinal plants used in South Africa

Soyingbe¹,

Mongalo²,

Makhafola³

2018

BMC Complement Altern Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…The essential oils of both the fresh and dry leaves of E. grandis showed considerable activities against respiratory tract bacteria. Various respiratory pathogens affect the respiratory tracts which lead to oxidative stress which in turn triggers asthmatic attack (Soyingbe et al 2013) by destroying the membrane integrity of the bacteria and also blocking the efflux pump mechanism of the bacteria. It is concluded that the essential oils could be used as part of the existing anti-asthma therapy, and also justify its rationale use by traditional healer in the treatment of asthma.…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial and efflux pumps inhibitory activities of Eucalyptus grandis essential oil against respiratory tract infectious bacteria

Soyingbe¹,

Myeni²,

Osunsanmi³

et al. 2015

J. Med. Plants Res.

Self Cite

View full text Add to dashboard Cite

Essential oils obtained by hydrodistillation from the fresh and dry leaves of Eucalyptus grandis were analyzed by gas chromatography (GC) and gas chromatography mass spectrometry (GC-MS). The main components of the fresh leaf oil were α-pinene (29.6%), p-cymene (19.8%), 1,8-cineole (12.8%) and αterpineol (6.4%). While, the dry leaf oil had 1,8-cineole (47.4%), limonene (13.3%), α-pinene (7.5%) and spathulenol (7.1%). The antimicrobial activities of the essential oils were tested against respiratory tract infectious microorganisms (Klebsiella pneumoniae, Staphylococcus aureus and Moraxella catarrhalis) using the microdilution-broth methods. The minimum inhibitory concentration and minimum bactericidal concentration values of the oils ranged between (0.31 to 1.25) mg/ml and (0.63->5) mg/ml respectively. The minimum bactericidal concentration values caused the release of cytosolic lactate dehydrogenase (membrane damage) which ranges from 8 to 24% in comparison with Triton-X-100. The accumulation of rhodamine 6G in bacterial cells showed that the essential oils were effective as efflux pump inhibitors. The results of this study support the use of the plant in folk medicine.

show abstract

“…The cytosolic lactate dehydrogenase assay was carried out according to the methodology described by Soyingbe et al . [[ 24 ]] with some modifications. Standardized test Listeria cultures matching 0.5 MacFarland standard were grown for 18-24 h in a concentration of 4× MIC value of each triterpene after which, the mixture was centrifuged (5 000 × g for 5 mins).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the anti-Listeria potentials of some plant-derived triterpenes

Penduka

Mosa

Simelane

et al. 2014

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

BackgroundListeriosis is a fatal disease caused by pathogenic Listeria bacteria and it is most prevalent in immune-compromised individuals. The increase in numbers of immune-compromised individuals against a background of Listeria antibiotic resistance, limits listeriosis treatment options. This therefore calls for research into substitute treatments, of which, medicinal plants derived compounds offer a viable alternative.MethodsThe broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of three plant triterpenes namely 3β-hydroxylanosta-9,24-dien-21-oic acid, methyl-3β-hydroxylanosta-9,24-dien-21-oate and 3β-acetylursolic acid, against Listeria monocytogenes, Listeria ivanovii and Listeria grayi species. The chequerboard method was used to assess the interactions between the triterpenes and conventional antibiotics: ampicillin, neomycin, gentamicin and penicillin G. The lactate dehydrogenase membrane damage method was used to assess the triterpenes’ membrane damaging potentials against the Listeria bacteria.ResultsThe triterpenes’ MIC values were found to range from 0.185 to 1.67 mg/ml while, the MBC determination assay results revealed that the test triterpenes were bacteriostatic against the Listeria bacteria. The interactions involving 3β-hydroxylanosta-9,24-dien-21-oic acid were mainly additive with ampicillin and synergistic with neomycin, gentamicin and penicillin G. The interactions involving methyl-3β-hydroxylanosta-9,24-dien-21-oate were mainly antagonistic with ampicillin, indifferent with neomycin, ranging from synergistic to indifference with gentamicin and synergistic with penicillin G. The interactions involving 3β-acetylursolic acid were mainly indifferent with ampicillin, synergistic with neomycin and gentamicin while ranging between synergistic and additive with penicillin G. The low levels of cytosolic lactate dehydrogenase released from the cells treated with 4× MIC concentration of the triterpenes in comparison to that of cells treated with 3% Triton X-100 proved that membrane damage was not the mode of action of the triterpenes.ConclusionThis study therefore shows the potential that these plant triterpenes have in listeriosis chemotherapy especially as shown by the favourable interactions they had with penicillin G, one of the antibiotics of choice in listeriosis treatment.

show abstract

The Essential Oil of <i>Eucalyptus grandis</i> W. Hill ex Maiden Inhibits Microbial Growth by Inducing Membrane Damage

Cited by 18 publications

References 23 publications

In vitro antibacterial and cytotoxic activity of leaf extracts of Centella asiatica (L.) Urb, Warburgia salutaris (Bertol. F.) Chiov and Curtisia dentata (Burm. F.) C.A.Sm - medicinal plants used in South Africa

In vitro antibacterial and cytotoxic activity of leaf extracts of Centella asiatica (L.) Urb, Warburgia salutaris (Bertol. F.) Chiov and Curtisia dentata (Burm. F.) C.A.Sm - medicinal plants used in South Africa

Antimicrobial and efflux pumps inhibitory activities of Eucalyptus grandis essential oil against respiratory tract infectious bacteria

Evaluation of the anti-Listeria potentials of some plant-derived triterpenes

Contact Info

Product

Resources

About