The Establishment and Application of a Kraken Classifier for <i>Salmonella</i> Plasmid Sequence Prediction

Abstract

Introduction: Salmonella is a key intestinal pathogen of foodborne disease, and the plasmids in Salmonella are related to many biological characteristics, including virulence and drug resistance. A large number of plasmid contigs have been sequenced in bacterial draft genomes, however, these are often difficult to distinguish from chromosomal contigs.Methods: In this study, three different customized Kraken databases were used to build three different Kraken classifiers. Complete genome benchmark datasets and simulated draft genome benchmark datasets were constructed. Five-fold cross-validation was used to evaluate the performance of the three different Kraken classifiers by two benchmark datasets.Results: The predictive performance of the classifier based on all National Center for Biotechnology Information plasmids and Salmonella complete genomes was optimal. This optimal Kraken classifier was performed with Salmonella isolated in China. The plasmid carrying rate of Salmonella in