The Establishment and Application of Indirect 3AB-ELISA for the Detection of Antibodies against Senecavirus A

Abstract

Senecavirus A (SVA) is an emerging pathogen that negatively affects the pig industry in China. Affected animals present vesicular lesions which are indistinguishable from other vesicular diseases. To date, there is no commercial vaccine that can be used to control SVA infection in China. In this study, recombinant SVA 3AB, 2C, 3C, 3D, L and VP1 proteins are expressed by using a prokaryotic expression system. The kinetics of the presence and levels of SVA antibodies with SVA-inoculated pig serum show that 3AB has the best antigenicity. An indirect enzyme-linked immunosorbent assay (ELISA) is developed with the 3AB protein, exhibiting a sensitivity of 91.3% and no cross-reaction with serum antibodies against PRRSV, CSFV, PRV, PCV2 or O-type FMDV. Given the high sensitivity and specificity of this approach, a nine-year (2014–2022) retrospective and prospective serological study is conducted to determine the epidemiological profile and dynamics of SVA in East China. Although SVA seropositivity declined markedly from 2016 (98.85%) to 2022 (62.40%), SVA transmission continues in China. Consequently, the SVA 3AB-based indirect ELISA has good sensitivity and specificity and is suitable for viral detection, field surveillance and epidemiological studies.

Real-time detection of Seneca Valley virus by one-tube RPA-CRISPR/Cas12a assay

“…The symptoms observed in pigs infected by SVA resemble those caused by vesicular stomatitis virus (VSV) and foot-and-mouth disease virus (FMDV), leading to difficulties in clinical differential diagnosis ( Schmitt, 2002 ; Knight-Jones et al., 2016 ). Currently, many methods such as PCR-based assays and ELISA have been established for the laboratory diagnosis of SVA ( Bracht et al., 2016 ; Feronato et al., 2018 ; Mu et al., 2020 ; Ma Z. et al., 2022 ; Yan et al., 2023 ). However, these methods are cumbersome, complex and time-consuming.…”