The Establishment and Use of an in vivo Animal Model for Cervical Intra-Epithelial Neoplasia

Abdul, Ahmad Bustamam; Abdullah, M.; Ibrahim, Siddiq; Tailan, Nirmala Devi; Al-Zubairi, Adel Sharaf; Mohan, Murali Mohan Syam

doi:10.3923/ijcr.2008.61.70

Cited by 13 publications

(8 citation statements)

References 21 publications

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“…Zerumbone is known to exhibit an antiproliferative effect on human cervical cancer (HeLa) cell line [ 87 ]. In diethylstilboestrol- (DES-) induced mice cervical interepithelial neoplasia (CIN), ZER caused overexpression of proapoptotic protein, Bax [ 88 , 130 ].…”

Section: Anticancer Properties Of Zerumbonementioning

confidence: 99%

Biomedical Properties of a Natural Dietary Plant Metabolite, Zerumbone, in Cancer Therapy and Chemoprevention Trials

Rahman

Abdullah

Yeap

et al. 2014

BioMed Research International

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Zerumbone (ZER) is a naturally occurring dietary compound, present in many natural foods consumed today. The compound derived from several plant species of the Zingiberaceae family that has been found to possess multiple biomedical properties, such as antiproliferative, antioxidant, anti-inflammatory, and anticancer activities. However, evidence of efficacy is sparse, pointing to the need for a more systematic review for assessing scientific evidence to support therapeutic claims made for ZER and to identify future research needs. This review provides an updated overview of in vitro and in vivo investigations of ZER, its cancer chemopreventive properties, and mechanisms of action. Therapeutic effects of ZER were found to be scientifically plausible and could be explained partially by in vivo and in vitro pharmacological activities. Much of the research outlined in this paper will serve as a foundation to explain ZER anticancer bioactivity, which will open the door for the development of strategies in the treatment of malignancies using ZER.

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Section: Anticancer Properties Of Zerumbonementioning

confidence: 99%

Biomedical Properties of a Natural Dietary Plant Metabolite, Zerumbone, in Cancer Therapy and Chemoprevention Trials

Rahman

Abdullah

Yeap

et al. 2014

BioMed Research International

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“…The genotoxic effects of the combination of test compounds, ZER and 2.5 μM and 5 μM Cisplatin were tested. The tested concentrations were chosen according to the cytotoxicity tests [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28]. The results of the present study demonstrated that the frequency of breaks, gaps, fragments, dicentrics, and endoreduplication was increased significantly in cultures of CHO cell lines treated with ZER alone and in combination with Cisplatin when compared with the control.…”

Section: Discussionmentioning

confidence: 95%

“…Many published researches have reported the antitumour effects of zerumbone in different cancer cell lines, including ovarian, colon, breast, myeloid, pancreatic, lung and gastric cancers [11][12][13][14][15][16][17][18][19]. Our previous work in vivo, we have reported the activity of ZER against DES-induced mice Cervical Intraepithelial Neoplasia (CIN) [20]. In addition, It has been reported to inhibit both azoxymethane-induced rat aberrant crypt foci and phorbol ester-induced papilloma formation in the mouse skin cancer [21].…”

Section: In Vitro Genotoxic Effects Of Zerumbone and Cisplatin Combination In Cho Cell Linesmentioning

confidence: 96%

In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines

Al-Zubairi

2017

Asian Pac J Cancer Biol

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Objective: Several natural products are being increasingly used in the treatment of cancer to minimize the adverse side effects of cancer chemotherapy. Zerumbone (ZER), the sesquiterpene derived from Zingiber zerumbet Smith, has been reported to have an in vitro anticancer effects against various human tumour cells as well as in vivo against a number of induced malignancies in mice. Previously we have reported the genotoxic effects of ZER in vitro against CHO cell lines. Material and Method: The aim of this study was to investigate the genotoxic effects of the combination of ZER along with cisplatin in CHO cells. Two cytogenetic endpoints were used, namely Chromosomal Aberrations assay (CA) and Micronucleus test (MN). Both cytogenetic endpoints were performed without any metabolic activation. Result: ZER treated cultures showed the significant increase in the frequency of the chromosome aberrations and MN induction. In CA assay, marked changes have been observed after co-treatment of CHO cell lines with different concentration of ZER along with 5 µM Cisplatin when compared to ZER treatment alone, suggesting a possible synergistic genetoxic effects. Whereas, treatment of CHO cell lines with different concentrations of ZER along with 2.5 µM Cisplatin was found to reduce chromosomal aberrations, suggesting an antagonistic genotoxic effect. On the other hand, in MN induction test, co-treatment of CHO cell lines with both 2.5 µM and 5 µM Cisplatin and different concentration of ZER found to reduce the genotoxic effects compared to the 2.5 µM and 5 µM Cisplatin alone suggesting an antagonistic genotoxic effect.Conclusion: The genotoxic effects of combined low concentrations of Cisplatin with different concentrations of ZER could have an antagonist genotoxic potential in vitro in CHO cell lines.

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“…The rhizomes have been known in Asian traditional medicine for treatment of many diseases and in particular for treatment of many gastrointestinal tract conditions (Jang et al, 2004;Prakash et al, 2011). Rhizomes extracts have been found to exhibit antispasmodic and anticonvulsant (Yob et al, 2011), anti-inflammatory (Sulaiman et al, 2010;Zakaria et al, 2010), anticancer (Rashid and Pihie, 2005;Bustamam et al, 2008;Abdul et al, 2008a), antibacterial (Abdul et al, 2008b;Kader et al, 2011), antipyretic (Somchit et al, 2005), antidiabetic (Tzeng et al, 2013), antioxidant (Habsah et al, 2000), antitumour (Wahab et al, 2008) and antiulcer (Al-Amin et al, 2012) effects, while the young shoots have been used as condiments. The main active constituents of the volatile oils of the rhizomes were found to contain zerumbone, humuline and camphene (Jang et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, Zerumbone has been reported to modulate osteoclastogenesis induced by RANKL and breast cancer (Sung et al, 2009) as well as carcinogenesis process suppression in mouse colon and lung (Kim et al, 2009). Our previous in vivo studies have shown zerumbone to have an activity against Cervical Intraepithelial Neoplasia (CIN) in mice (Bustamam et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Anti-Proliferative Activity of Zerumbone Against Tumour Cell Lines

Al-Zubairi¹

2018

OnLine Journal of Biological Sciences

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Zerumbone (ZER) is a cytotoxic phytochemical component extracted from the tropical herbal plant, Zingiber Zerumbet smith. It has been identified as an active anticancer compound. This study was aimed to evaluate in vitro the anti-proliferative activity of this bioactive compound, zerumbone, on seven cancer cell lines namely: CEMss (T4-lymphoplastoid), Chinese Hamster Ovary (CHO), 3T3 (mouse fibroblast), WEHI-3 (murine monomyelocytic leukemia), HeLa (Human cervical cancer), MCF-7 (human breast cancer) and MDA-MB-231 (human mammary cancer-estrogen negative). The antiproliferative activity of ZER was evaluated using 3-[4,5-dimethylthizol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, together with the commercially available anticancer drug Cisplatin as a control drug. The most sensitive cells to ZER were found to be WEHI-3B, 3T3 and Hela cell lines with an IC 50 of 5, 6 and 6.4 µg mL −1 , respectively, followed by CEMss cell lines with an IC 50 of 12 µg mL −1 . This indicates the possible potential use of ZER for treatment of hematological cancers in addition to the cervical cancer. Meanwhile, ZER was found to be less toxic to the normal cell lines CHO.

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The Establishment and Use of an in vivo Animal Model for Cervical Intra-Epithelial Neoplasia

Cited by 13 publications

References 21 publications

Biomedical Properties of a Natural Dietary Plant Metabolite, Zerumbone, in Cancer Therapy and Chemoprevention Trials

Biomedical Properties of a Natural Dietary Plant Metabolite, Zerumbone, in Cancer Therapy and Chemoprevention Trials

In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines

Anti-Proliferative Activity of Zerumbone Against Tumour Cell Lines

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