The Establishment of Colonial Variants in Guinea Pigs Infected with Smooth Brucella Suis

Redfearn, M.; Simon, Ellen M.; Berman, David T.

doi:10.1093/infdis/99.3.234

Cited by 4 publications

(2 citation statements)

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“…In both cases immunity elicited by infection with smooth brucellae was effective against non-smooth variants. This is further evidence against a role for non-smooth variants as a reservoir of chronic infection (18,20), despite their selective advantage in sera from Brucello-infected animals (21). If the reticuloendothelial system of chronically infected animals behaves in the intact animal as monocytes from immunized animals do in vitro, a small proportion of phagocytes would allow multiplication of intracellular bruceUae.…”

Section: Discussionmentioning

confidence: 81%

“…Similarly, we could not elicit a positive skin test in BruceUa-infected mice, using either brucellergen or dead Brucella cell antigens. (17), although there is a definite cross-reaction (delayed hypersensitive skin reaction) to mucoid variant antigens in guinea pigs infected with the smooth type (18). The specificity of growth inhibition by "immune" monocytes was investigated by comparing intracellular growth of smooth and non-smooth variants of B. suis.…”

Section: Exper 17ffi~-ntal Observationsmentioning

confidence: 99%

See 1 more Smart Citation

A Cellular Basis of Immunity in Experimental Brucella Infection

Holland

Pickett

1958

The Journal of Experimental Medicine

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Brucellae are known to be facultative intracelluiar parasites (1, 2). Braude (2) showed that within 24 hours after infection of guinea pigs with Brucdla abortus, nearly all brucellae detectable in the peripheral blood were within neutrophiles. These infected phagocytes (and extracellular bacteria) were removed from the circulation by the spleen, liver, and other organs. Focal aggregations of parasitized polymorphonuclear and mononuclear phagocytes in these organs led to development of granulomas. Phagocytized brucellae were not destroyed but rather appeared to multiply intracellularly until many phagocytes were completely engorged. With onset of the mature granuloma, marked by the appearance of epithelioid cells, microscopically evident brucellae gradually disappeared from the tissues.Intracellular multiplication of Brucella in vitro has recently been demonstrated (3, 4). Similar methods (5-7), and methods involving passive transfer of cells or humoral factors (8, 9), have been applied to the study of acquired immunity in tuberculosis, but with equivocal results. Acquired resistance in brucellosis is much more amenable to investigation because high titer agglutinating antibody can be produced, and because reproducible viability counts from infected cells and tissues are possible.The studies reported here were initiated to determine the reasons for the restricted multiplication of brucellae within phagocytes of animals previously infected with Brucella. While this work was in progress, others (4, 10) reported that monocytes from vaccinated guinea pigs and rabbits restricted intracellular growth of brucellae in a cell culture system, whereas marked multiplication occurred within normal monocytes under the same conditions. Our findings confirm and extend their results. Materials and MethodsAnimals.--Three species were used: male guinea pigs (350 to 500 gm.), female rats of the Sprague-Dawley strain (250 gin.), and young adult (about 20 gin.) mice of both sexes (inbred progeny of LAFa strain of mice).

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Section: Discussionmentioning

confidence: 81%

Section: Exper 17ffi~-ntal Observationsmentioning

confidence: 99%