The establishment of pancreatic islet isolation in India – an update on human pancreatic islet transplantation

Mohan, Nithyakalyani; Raghavan, Srividhya; Sunder, Anusha; Vaidya, Anil; Chandrasekaran, Nivash; Muthuraman, Senthil; Babu, Dinesh; Mathivanan, Karthik; Krishnan, Elan; Appakalai, Balamurugan

doi:10.53388/lr20220027

Cited by 2 publications

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“…While for isolated islet cells, the islet preparation itself can show variability with parameters such as, isolation stress, culture conditions and duration. [19][20][21][22][23][24][25][26].…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Analysis to Study the Histological and Immunohistochemical Profile of Diabetic and Non-Diabetic Human Pancreases in the Indian Population

Mohan,

Sunder,

Himakar Reddy

2024

J Pathol Res Rev Rep

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The pathogenesis of hyperglycemia observed in most forms of diabetes is intimately tied to the islet β cell. Impairments in pro-peptide processing and secretory function, along with the loss of these vital cells, is demonstrable not only in those in whom the diagnosis is established but typically also in individuals who are at increased risk of developing the disease. Due to ethical and practical difficulties, genomic or pathological data in Indian population are remarkably missing. Here we report the immunohistological observations for 21 pancreases. We analyzed the pathological differences between diabetic and non-diabetic samples along with pathological changes associated with beta cell density, expression, size, and diameter. This study provides an insight into the immunopathological and histochemical mechanisms that highlight a number of–inflammatory, immunoregulatory and regenerative pathways, some of which have received relatively little attention so far.

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Section: Discussionmentioning

confidence: 99%

A Comprehensive Analysis to Study the Histological and Immunohistochemical Profile of Diabetic and Non-Diabetic Human Pancreases in the Indian Population

Mohan,

Sunder,

Himakar Reddy

2024

J Pathol Res Rev Rep

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“…Human islet isolation and transplantation has been performed in several Centers around the World over the past 20 years [ 1 – 5 ]. Islet transplanted type 1 diabetes mellitus (T1D) patients present marked improvement in both short-term and long-term outcomes, with insulin independence after initial or subsequent transplantation being achieved in up to 80% of the patients one year post-transplantation [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Approximately 50% of the patients remained insulin-independent at five years after receiving the transplant. Islet transplantation is already approved and reimbursable by insurance companies or covered by National Health Systems in several countries, including Canada, Australia, the United Kingdom, Switzerland, Italy, France and other parts of Europe, in total of 40 countries approximately [ 3 , 5 ]. Over 2,800 procedures have been performed worldwide in 1,399 recipients [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Islet transplantation: overcoming the organ shortage

Mantovani

Gabanyi

Pantanali

et al. 2023

Diabetol Metab Syndr

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Background Type 1 diabetes mellitus (T1D) is a condition resulting from autoimmune destruction of pancreatic β cells, leading patients to require lifelong insulin therapy, which, most often, does not avoid the most common complications of this disease. Transplantation of isolated pancreatic islets from heart-beating organ donors is a promising alternative treatment for T1D, however, this approach is severely limited by the shortage of pancreata maintained under adequate conditions. Methods In order to analyze whether and how this problem could be overcome, we undertook a retrospective study from January 2007 to January 2010, evaluating the profile of brain-dead human pancreas donors offered to our Cell and Molecular Therapy NUCEL Center (www.usp.br/nucel) and the basis for organ refusal. Results During this time period, 558 pancreata were offered by the São Paulo State Transplantation Central, 512 of which were refused and 46 were accepted for islet isolation and transplantation. Due to the elevated number of refused organs, we decided to analyze the main reasons for refusal in order to evaluate the possibility of improving the organ acceptance rate. The data indicate that hyperglycemia, technical issues, age, positive serology and hyperamylasemia are the top five main causes for declination of a pancreas offer. Conclusions This study underlines the main reasons to decline a pancreas offer in Sao Paulo—Brazil and provides some guidance to ameliorate the rate of eligible pancreas donors, aiming at improving the islet isolation and transplantation outcome. Trial registration: Protocol CAPPesq number 0742/02/CONEP 9230.

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The establishment of pancreatic islet isolation in India – an update on human pancreatic islet transplantation

Cited by 2 publications

References 0 publications

A Comprehensive Analysis to Study the Histological and Immunohistochemical Profile of Diabetic and Non-Diabetic Human Pancreases in the Indian Population

A Comprehensive Analysis to Study the Histological and Immunohistochemical Profile of Diabetic and Non-Diabetic Human Pancreases in the Indian Population

Islet transplantation: overcoming the organ shortage

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