The Esterase Activity in Megaloblasts, Leukaemic and Normal Haemopoietic Cells

Rozenszajn, L. A.; Leibovich, M.; Shoham, D.; Epstein, J.

doi:10.1111/j.1365-2141.1968.tb00366.x

Cited by 87 publications

(24 citation statements)

References 11 publications

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“…As noted in Table 1, by 18 h of oxygen exposure, SOD activity increased twofold in the 16,000-g pellet and threefold in the 100,000-g pellet. There was no significant increase in SOD activity in the 100,000-g supernate.…”

Section: Resultsmentioning

confidence: 82%

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The alteration of superoxide dismutase, catalase, glutathione peroxidase, and NAD(P)H cytochrome c reductase in guinea pig polymorphonuclear leukocytes and alveolar macrophages during hyperoxia.

Rister¹,

Baehner²

1976

J. Clin. Invest.

173

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A B S T R A C T Superoxide dismutase, catalase, glutathione peroxidase, and NAD(P)H cytochrome c reductase were quantitated in polymorphonuclear leukocytes (PMN) and alveolar macrophages (AM) obtained from guinea pigs exposed up to 90 h to 85% oxygen. PMN and AM were sonicated and separated into a 16,000-g pellet, a 100,000-g pellet, and a 100,000-g supernate. Superoxide dismutase activity increased in both cells within 18 h, persisted for 66 h and decreased by 90 h. The highest rate of increase was in the 100,000-g pellet containing 3.4% of total enzyme activity in PMN but 28% in AM. The enzyme induction in PMN and AM was partially inhibited by daily intracardiac injections of 50 mg/kg actinomycin D.During oxygen exposure, catalase activity in PMN and AM decreased to 60% of its original activity, and glutathione peroxidase was reduced in PMN to 60% and in AM to 20% of control values. Although NAD(P)H cytochrome c reductase decreased to 50% in PMN, no change was noted in AM. Upon exposure to superoxide anion, purified catalase, the glutathione peroxidase of the 100,000-g supernate, NADH, and NADPH cytochrome c reductases of the 16,000-g Dr. Rister is a recipient of Deutsche Forschungsgemeinschaft.

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Section: Resultsmentioning

confidence: 82%

“…The increased SOD activity in AM cannot be explained by an inflammatory process or lung infection. Histologic and microbiologic studies of the lung by 18 h exhibited no difference between animals exposed to air or to an FI 02 of 85%.…”

Section: Resultsmentioning

confidence: 98%

The alteration of superoxide dismutase, catalase, glutathione peroxidase, and NAD(P)H cytochrome c reductase in guinea pig polymorphonuclear leukocytes and alveolar macrophages during hyperoxia.

Rister¹,

Baehner²

1976

J. Clin. Invest.

173

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“…* The classification of leukemias was according to morphologic and cytochemical parameters used in the literature (11,12).…”

Section: Methodsmentioning

confidence: 99%

Interaction of peanut agglutinin with normal human lymphocytes and with leukemic cells.

Reisner¹,

Biniaminov²,

Rosenthal³

et al. 1979

Proc. Natl. Acad. Sci. U.S.A.

119

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The interaction of peanut agglutinin (PNA) with human thymocytes, peripheral blood Imphocytes, and peripheral blood cells of various types of leuiemia was investigated by using fluorescein isothiocyanate-conjugated PNA. The majority of human thymocytes (60-80%) bind the lectin.The major subpopulation of thymocytes that is PNA-positive was separated from the PNA-negative cells by differential agglutination with the lectin. The two thymocyte subpopulations were tested in the mixed lymphocyte reaction and with the phytohemagglutinin of Phaseolus vulgaris. The poor response of the PNA-positive thymocytes to these stimuli indicates that these thymocytes are functionally immature. The fluorescein isothiocyanate-PNA-binding test with peripheral blood lymphocytes of leukemic patients revealed that in most acute leukemias the PNA receptor is exposed on the blastic cells, whereas in most cases of chronic leukemia the peripheral blood lymphocytes are PNA-negative. The validity of PNA as a marker of immature blood cells and its potential clinical application are discussed.Classification of the various leukemias according to the cellular origin of their pathologic cells is of diagnostic and therapeutic importance. However, such a classification is hampered by the scarcity of cell markers that can be attributed to lymphoid or myeloid subpopulations at specific stages of their differentiation (1). It has recently been shown that peanut agglutinin (PNA) binds exclusively to undifferentiated murine lymphocytes such as immature hydrocortisone-sensitive murine thymocytes (2), fetal liver lymphocytes (3), and bone marrow and spleen stem cells (4). On mature lymphocytes the lectin receptor is masked by sialic acid and can be exposed upon treatment of the cells with neuraminidase.In the present study we have investigated the interaction of PNA with human thymocytes, peripheral blood lymphocytes, and various types of leukemic blast cells. The validity of PNA as a marker of immature human blood cells and its potential for clinical application are discussed. MATERIALS AND METHODSPNA was purified by affinity chromatography on a column of Sepharose-N-(e-aminocaproyl)-fl-D-galactopyranosylamine (5). Fluorescein isothiocyanate (FITC) was conjugated with PNA as described (6), and the conjugated lectin was repurified by affinity chromatography. 125I-labeled PNA (125I-PNA) was prepared as described (2), and the iodinated lectin was purified on a Sephadex G-150 column followed by affinity chromatography. Phytohemagglutinin (PHA) was obtained from Wellcome-Burroughs. [methyl-3H]Thymidine (5 Ci/mmol; 1 Ci = 3.7 X 10l°becquerels) was obtained from the Nuclear Research Center, Negev, Israel. Neuraminidase of Vibrio cholera was obtained from Behringwerke, Marburg, Germany; 1 unit is defined as the amount of enzyme that liberates 1 ,ug of N-acetylneuraminic acid from a1-glycoprotein within 15 min at pH 5.5 and 370C.Cell Preparations. Normal peripheral blood lymphocytes were purified from heparinized peripheral blood by centrifugation over Fic...

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“…Supporting this view is evidence from our cytochemical study that these cells exhibit a strong activity for the enzyme non-specific esterase (KAUR, 1987). The latter has been shown to be specific for blood monocytes (ROZENSZAJN et al, 1968;SCHMALZL and BRAUNSTEINER, 1970;YAM et al, 1971;LI et al, 1973;HORWITZ et al, 1977;PAYNE et al, 1980;KAUR, 1987). Besides non-specific esterase, these cells show a positive reaction for the enzymes thiamine pyrophosphatase, N-acetyl-glucosaminidase and acid phosphatase (KAUR, 1987).…”

Section: Discussionmentioning

confidence: 99%

Scanning electron microscopy of transitory subependymal cysts in the developing midbrain of postnatal rats.

Kaur

Ling

Wong

1989

Archives of Histology and Cytology

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The Esterase Activity in Megaloblasts, Leukaemic and Normal Haemopoietic Cells

Cited by 87 publications

References 11 publications

The alteration of superoxide dismutase, catalase, glutathione peroxidase, and NAD(P)H cytochrome c reductase in guinea pig polymorphonuclear leukocytes and alveolar macrophages during hyperoxia.

The alteration of superoxide dismutase, catalase, glutathione peroxidase, and NAD(P)H cytochrome c reductase in guinea pig polymorphonuclear leukocytes and alveolar macrophages during hyperoxia.

Interaction of peanut agglutinin with normal human lymphocytes and with leukemic cells.

Scanning electron microscopy of transitory subependymal cysts in the developing midbrain of postnatal rats.

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