I would like to acknowledge several people and institutions that support myself during these years and made this dream came true. First, I am sincerely and deeply grateful with Professor Clive Phillips, who has been an invaluable support during the whole PhD. Clive provided to me guidance, comprehensive review and correction of materials, but most importantly, thank you for teaching me humility, productivity and the importance of to be an excellent and close leader of students. I am also grateful to my co-supervisor Dr Carmen Gallo S., for technical advice and support during the Chilean experiment and for her always useful ideas. I am grateful to Dr Ramazan Col, for his work and support during the data collection during the simulated sea transport trials. Furthermore, I would like to thank you to Blandine Fabre, Elise Wicker and Alice Guingal for their assistance with the laboratory work. I also thank the Queensland Animal Science Precinct (QASP) staff for their assistance during the trial preparation, particularly to Andrew Kelly. I am sincerely grateful to the School of Veterinary Science staff for their support, especially to Annette Winter for her administrative help and to Rod Verrall and Carissa Reinke for assisting me with obtaining the sheep, as well as handling and transporting them. Additionally, I would like to thank you to all the CAWE students for their emotional support and friendship for almost 4 years. Being an international student, the Brisbane friends become your family. I am sincerely grateful for the friendship of Karen