The ethylene-responsive transcription factor of durum wheat, TdSHN1, confers cadmium, copper, and zinc tolerance to yeast and transgenic tobacco plants

Djemal, Rania; Khoudi, Habib

doi:10.1007/s00709-021-01635-z

Cited by 32 publications

(13 citation statements)

References 98 publications

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“…Jasmonic acid-deficient tomato mutant def-1 exposed to salinity stress (100 mM NaCl) showed a two-fold increase in H 2 O 2 level along with reduced plant growth parameters, including shoot (3.28 g) and root (0.72 g) dry weights, shoot length (9.04 cm), and leaf number (5.83) under treatment ( Abouelsaad and Renault, 2018 ). Djemal and Khoudi (2022) showed that ethylene-responsive transcription factor TdSHN1 from durum wheat ( Triticum durum ) conferred Cd, Cu, and Zn tolerances in transgenic yeast and tobacco. The transgenic tobacco lines exhibited more significant biomass accumulation and longer roots, reserved more Chl, and formed less ROS than WT plants.…”

Section: Transgenic and Crispr/cas-based Solution For Crop Improvementmentioning

confidence: 99%

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Plant hormones and neurotransmitter interactions mediate antioxidant defenses under induced oxidative stress in plants

et al. 2022

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Due to global climate change, abiotic stresses are affecting plant growth, productivity, and the quality of cultivated crops. Stressful conditions disrupt physiological activities and suppress defensive mechanisms, resulting in stress-sensitive plants. Consequently, plants implement various endogenous strategies, including plant hormone biosynthesis (e.g., abscisic acid, jasmonic acid, salicylic acid, brassinosteroids, indole-3-acetic acid, cytokinins, ethylene, gibberellic acid, and strigolactones) to withstand stress conditions. Combined or single abiotic stress disrupts the normal transportation of solutes, causes electron leakage, and triggers reactive oxygen species (ROS) production, creating oxidative stress in plants. Several enzymatic and non-enzymatic defense systems marshal a plant’s antioxidant defenses. While stress responses and the protective role of the antioxidant defense system have been well-documented in recent investigations, the interrelationships among plant hormones, plant neurotransmitters (NTs, such as serotonin, melatonin, dopamine, acetylcholine, and γ-aminobutyric acid), and antioxidant defenses are not well explained. Thus, this review discusses recent advances in plant hormones, transgenic and metabolic developments, and the potential interaction of plant hormones with NTs in plant stress response and tolerance mechanisms. Furthermore, we discuss current challenges and future directions (transgenic breeding and genome editing) for metabolic improvement in plants using modern molecular tools. The interaction of plant hormones and NTs involved in regulating antioxidant defense systems, molecular hormone networks, and abiotic-induced oxidative stress tolerance in plants are also discussed.

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Section: Transgenic and Crispr/cas-based Solution For Crop Improvementmentioning

confidence: 99%

“…The transgenic tobacco lines exhibited more significant biomass accumulation and longer roots, reserved more Chl, and formed less ROS than WT plants. In addition, higher activities of ROS-scavenging enzymes (SOD and CAT) contributed to heavy metal tolerance ( Djemal and Khoudi, 2022 ).…”

Section: Transgenic and Crispr/cas-based Solution For Crop Improvementmentioning

confidence: 99%

Plant hormones and neurotransmitter interactions mediate antioxidant defenses under induced oxidative stress in plants

et al. 2022

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“…These genes have been reported to be involved in different processes. Djmal and Khoudi [ 40 ] studied the tolerance to heavy metals (HM) regulated by the durum wheat TdSHN1 in yeast and transgenic tobacco, concluding that the gene could improve HM tolerance in plants and phytoremediation of HM-contaminated soils. Gao et al [ 41 ] studied the expression of the wheat ERF TaERFL1a .…”

Section: Discussionmentioning

confidence: 99%

Discovering Loci for Breeding Prospective and Phenology in Wheat Mediterranean Landraces by Environmental and eigenGWAS

Yannam

Rufo

Marcotuli

et al. 2023

IJMS

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Knowledge of the genetic basis of traits controlling phenology, differentiation patterns, and environmental adaptation is essential to develop new cultivars under climate change conditions. Landrace collections are an appropriate platform to study the hidden variation caused by crop breeding. The use of genome-wide association analysis for phenology, climatic data and differentiation among Mediterranean landraces led to the identification of 651 marker-trait associations that could be grouped in 46 QTL hotspots. A candidate gene analysis using the annotation of the genome sequence of the wheat cultivar ‘Chinese Spring’ detected 1097 gene models within 33 selected QTL hotspots. From all the gene models, 42 were shown to be differentially expressed (upregulated) under abiotic stress conditions, and 9 were selected based on their levels of expression. Different gene families previously reported for their involvement in different stress responses were found (protein kinases, ras-like GTP binding proteins and ethylene-responsive transcription factors). Finally, the synteny analysis in the QTL hotspots regions among the genomes of wheat and other cereal species identified 23, 21 and 7 ortho-QTLs for Brachypodium, rice and maize, respectively, confirming the importance of these loci.

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“…Yeast is an important tool for screening genes involved in abiotic stress resistance. For example, transference of ThvhAC1 gene from Tamarix hispida into yeast cells improved tolerance and survival of transgenic yeast under abiotic stress [ 34 ], while overexpression of TdSHN1 ( Durum wheat gene) improved tolerance to Cd, Cu, and Zn stress in transgenic yeast [ 35 ]. In this study, PtrDHN-3 improved tolerance to salt stress in the transformed yeast cells ( Figure 4 ), although there was no obvious difference under mannitol-induced drought stress (data not shown).…”

Section: Discussionmentioning

confidence: 99%

The Poplar (Populus trichocarpa) Dehydrin Gene PtrDHN-3 Enhances Tolerance to Salt Stress in Arabidopsis

Zhou

Peng

Yang

et al. 2022

Plants

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Dehydrin (DHN), a member of the late embryogenesis abundant protein (LEA) family, was recently found to play a role in physiological responses to salt and drought stress. In this study, we identified and cloned the PtrDHN-3 gene from Populus trichocarpa. The PtrDHN-3 protein encoded 226 amino acids, having a molecular weight of 25.78 KDa and an isoelectric point of 5.18. It was identified as a SKn-type DHN and was clustered with other resistance-related DHN proteins. Real-time fluorescent quantitative PCR showed that transcription levels of PtrDHN-3 were induced by mannitol stress, and more significantly by salt stress. Meanwhile, in a yeast transgenic assay, salt tolerance increased in the PtrDHN-3 transgenic yeast, while the germination rate, fresh weight and chlorophyll content increased in PtrDHN-3-overexpressing transgenic Arabidopsis plants (OE) under salt stress. Significant increases in expression levels of six antioxidant enzymes genes, and SOD and POD enzyme activity was also observed in the OE lines, resulting in a decrease in O2- and H2O2 accumulation. The proline content also increased significantly compared with the wild-type, along with expression of proline synthesis-related genes P5CS1 and P5CS2. These findings suggest that PtrDHN-3 plays an important role in salt resistance in plants.

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The ethylene-responsive transcription factor of durum wheat, TdSHN1, confers cadmium, copper, and zinc tolerance to yeast and transgenic tobacco plants

Cited by 32 publications

References 98 publications

Plant hormones and neurotransmitter interactions mediate antioxidant defenses under induced oxidative stress in plants

Plant hormones and neurotransmitter interactions mediate antioxidant defenses under induced oxidative stress in plants

Discovering Loci for Breeding Prospective and Phenology in Wheat Mediterranean Landraces by Environmental and eigenGWAS

The Poplar (Populus trichocarpa) Dehydrin Gene PtrDHN-3 Enhances Tolerance to Salt Stress in Arabidopsis

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